42 results about "Metabolic biomarkers" patented technology

Beta-hydroxybutyrate mineral salts in combination with medium chain fatty acids or an ester thereof such as medium chain triglycerides were used to induce ketosis, achieving blood ketone levels of (2-7 mmol/L), with or without dietary restriction. The combination results in substantial improvements in metabolic biomarkers related to insulin resistance, diabetes, weight loss, and physical performance in a short period of time. Further, use of these supplements to achieve ketosis yields a significant elevation of blood ketones and reduction of blood glucose levels. Use of these substances does not adversely affect lipid profiles. By initiating rapid ketosis and accelerating the rate of ketoadaptation, this invention is useful for the avoidance of glucose withdrawal symptoms commonly experienced by individuals initiating a ketogenic diet, and minimizes the loss of lean body mass during dietary restriction.

Panels of serum metabolic biomarkers and methods of their use in detecting and diagnosing cancer, especially ovarian cancer, are disclosed. The metabolic biomarker panels include 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 50, 75, 100, 150, or more metabolites. Supervised classification methods, such as trained support vector machines (SVMs) are used to determine whether the levels of metabolic biomarkers in a subject are indicative of the presence of cancer. The disclosed biomarkers and methods preferably allow a diagnosis of cancer with an accuracy, a specificity, and/or a sensitivity of at least 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99%.

The present invention provides a novel oral cancer and periodontal disease salivary metabolome for use in the diagnosis or for providing a prognosis for oral cancer and periodontal disease in an individual. The present invention also provides novel methods of diagnosing or providing a prognosis for oral cancer or periodontal disease by detecting metabolites found in the saliva of an individual. Finally, the present invention provides kits for the detection of salivary metabolites useful in the diagnosis or prognosis of oral cancer and periodontal disease in an individual.

The present invention relates to a metabolic biomarker set for use in assessing breast cancer in a mammalian subject. In particular, the invention relates to a metabolic biomarker set for screening and/or diagnosing breast cancer, the metabolic biomarker set comprising at least (a) one amino acid selected from glutamine, glutamate and serine, and one lipid, or (b) glutamine and glutamate. Further, the invention relates to a metabolic biomarker set for prediction of therapeutic response to breast cancer neoadjuvant chemotherapy. Further, the invention relates to a metabolic biomarker set for assessing biochemical reflection of breast cancer tumor activity. Further, the invention relates to a metabolic biomarker set for subclassification between intrinsic breast cancer tumor subtypes. Moreover, the present invention relates to a method for assessing breast cancer, which comprises obtaining a biological sample, preferably blood, from a mammalian subject and measuring in the biological sample the amount and/or ratios of metabolites. By employing the specific biomarkers and the method according to the present invention it becomes possible to more properly and reliably assess breast cancer.

The invention provides for a method for identifying a biomarker in a fecal sample of a subject in need of such identification comprising: determining whether a fecal sample collected from a subject comprises a biomarker.

The invention relates to novel application of small molecule metabolites, namely hypoxanthine, 2-ketoisocaproic acid, glutamic acid and aspartic acid, in a serum sample as combined markers in preparation of a kit for distinguishing patients with esophageal squamous carcinoma and esophageal epithelial atypical hyperplasia in subjects. The invention further relates to a kit for detecting patients with esophageal squamous cell carcinoma in subjects. The relative concentration of the combined markers in serum samples from the subjects is detected, the variables P of the combined marker are calculated based on a linear regression equation, and whether the subjects suffer from esophageal squamous cell carcinoma or not is judged based on a determined threshold value. The kit can realize high-sensitivity and high-efficiency detection of several metabolites involved in the invention, and has the advantages of low detection cost and good repeatability. The kit can be applied to clinical auxiliary diagnosis of the esophageal squamous cell carcinoma, has the advantages of high diagnosis sensitivity and capability of effectively distinguishing the esophageal squamous cell carcinoma from precancerous lesions, and has a relatively good application prospect.

Disclosed is a method for detection of 24S-hydroxycholesterol in hair. More specifically, a trace amount of hair is hydrolyzed under basic condition and metabolites including 24S-hydroxycholesterol are purified and extracted by solid-phase extraction (SPE). Then, after derivatization of the resulting extract with trimethylsilyl (TMS), 24S-hydroxycholesterol is detected by gas chromatography-mass spectrometry (GC-MS).

A panel of metabolic biomarkers for differential diagnosis of stable angina pectoris and acute coronary syndrome is published herein. The panel comprises one or more of the metabolic biomarkers, including malic acid, taurine, arachidonic acid, citramalic acid, methionine, pentadecanoic acid. Single use of the 6 differential metabolites provided clinically diagnostic value of SA vs. ACS with AUC>0.7. When combined, the more metabolites, the larger of AUC. The highest AUC of 0.987 was obtained when all of the six metabolites were combined to distinguish SA vs. ACS with sensitivity 96.8% and specificity 97.7% using optimal cut-off value. The metabolic biomarkers provided by the invention can be used for differential diagnosis of SA vs. ACS of high accuracy, sensitivity and specificity.

Embodiments of the disclosure include methods and compositions for treatment of prostate cancer, including metastatic prostate cancer or prostate cancer at risk for developing into metastatic prostate cancer, by providing an effective therapy to an individual that has been determined to have elevated levels of SRC-2 (also known as NCOA2, GRIP1 TIF2). In particular cases, sample from an individual known to have prostate cancer is assayed for the risk for developing metastatic prostate cancer and the individual is provided an effective therapy upon determination of elevated levels of SRC-2.

The invention, which belongs to the technical field of gene engineering, provides a metabolic biomarker in a metabolic biomarker in a senescence process of sepiella maindroni. The metabolic biomarkercomprises uric acid. The screening method of the metabolic biomarker includes the following steps: collecting serum samples of individuals at different reproductive stages; preprocessing the serum samples; carrying out liquid chromatography-mass spectrometry analysis on the serum samples successively; screening LC-MS data processing to obtain metabolic biomarkers; carrying out comparison and identification on the metabolic biomarkers; and constructing a metabolic pathway of the biomarkers and carrying out analysis. According to the invention, micro metabonomics analysis is carried out on the serum of sepiella maindroni at different reproductive stages by using the high performance liquid chromatography-tandem mass spectrometry technology and screening is carried out; the content of uric acid is greatly reduced after spawning by being compared with the content before spawning. The following formulas are used: the content before spawning/the content after spawning=2.16; VIP=1.5385. The provided metabolic biomarker is a potential metabolic biomarker.

The present invention relates to new biomarkers for assessing ovarian cancer being more sensitive, particularly at early stage of disease. Moreover, the present invention relates to a method for assessing ovarian cancer from a patient to be examined, and to a kit for carrying out the method.

The invention discloses the use of a metabolic biomarker in acute pancreatitis. The invention is characterized by collecting normal acute pancreatitis and samples of drug therapy of the acute pancreatitis, performing metabolomics analysis, and discovering the metabolites which exhibit significant differences in different treatment groups, thereby using the metabolites to predict acute pancreatitis, and further achieving individualized treatment of patients.

The invention discloses a construction method of an autoimmune disease model based on serum metabolism fingerprints, and relates to the technical field of matrix-assisted laser desorption ionization mass spectrometry, biological sample metabolism molecule analysis and machine learning. Detecting and extracting serum metabolism fingerprints of the autoimmune diseases; constructing a model aiming at the autoimmune diseases by adopting a machine learning algorithm based on the serum metabolism fingerprint spectrum; and screening to obtain the metabolic biomarker. According to the method, the autoimmune disease model is successfully constructed based on the serum metabolism fingerprints by adopting a machine learning method. The defects of a nuclear magnetic resonance technology, a traditional mass spectrum technology (GC/LC-MS) and the like are effectively overcome through nano-assisted laser desorption ionization mass spectrometry (nano-assisted LDI MS), and rapid, high-throughput and high-sensitivity detection of the metabolic fingerprints of the serum low-molecular-weight segment of the autoimmune diseases is effectively achieved.