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Application of combined metabolism biomarker for diagnosing esophageal squamous carcinoma and kit

An esophageal squamous cell carcinoma and combined technology, which is applied in the fields of analytical chemistry, biochemistry and clinical medicine, can solve the problems of limiting the monitoring of high-risk groups for early detection and early diagnosis of esophageal squamous cell carcinoma, and achieves low detection cost, efficient detection, and high diagnostic sensitivity. Effect

Pending Publication Date: 2021-05-28
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are currently no clinical biomarkers that can effectively distinguish the two, and only pathological detection can distinguish the two, which greatly limits the early detection and early diagnosis of esophageal squamous cell carcinoma and the monitoring of high-risk groups
There is no report on the combination of these four metabolites in the diagnosis of esophageal squamous cell carcinoma

Method used

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  • Application of combined metabolism biomarker for diagnosing esophageal squamous carcinoma and kit
  • Application of combined metabolism biomarker for diagnosing esophageal squamous carcinoma and kit
  • Application of combined metabolism biomarker for diagnosing esophageal squamous carcinoma and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Serum Sample Collection

[0031] All volunteers involved in the present invention signed an informed consent form before serum sample collection.

[0032] Serum samples from 40 patients with esophageal squamous cell carcinoma and 33 patients with esophageal epithelial atypical hyperplasia were collected under the same collection conditions and set as the training set. All patients were confirmed by histopathology. The collected blood samples were left to stand for 30 minutes, then centrifuged at 4000 rpm for 10 minutes, and the supernatant serum was collected and stored in a -80°C refrigerator.

[0033] 2. Analysis method

[0034] 2.1 Serum sample pretreatment

[0035]Serum samples taken from -80°C were thawed at 4°C, vortexed for 10 seconds, 50 μL of serum was taken, and 200 μL of cold methanol containing 10 μg / mL tridecanoic acid (also known as tridecanoic acid) was added ( 0°C), vortexed for 1 minute and placed in an ice-water bath for 10 minutes. Next, the s...

Embodiment 2

[0046] 1. Serum Sample Collection

[0047] Sample collection method is the same as Example 1. Example 2 includes 34 cases of esophageal squamous cell carcinoma and 39 cases of atypical hyperplasia of esophageal epithelium, and these samples are set as a validation set.

[0048] 2. Analysis method

[0049] With embodiment 1.

[0050] 3. Analysis of serum verification results and diagnostic potential

[0051] The verification results of Example 2 are basically consistent with the training set data of Example 1. Specific results such as figure 1 shown. Using linear regression to combine hypoxanthine, 2-ketoisocaproic acid, glutamic acid and aspartic acid, the results are as follows figure 1 , figure 2 B. image 3 As shown, the area under the curve, sensitivity and specificity are high, and the discrimination effect of each group is good, which has a good application prospect.

[0052] The present invention also relates to a kit for detecting patients with esophageal squ...

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Abstract

The invention relates to novel application of small molecule metabolites, namely hypoxanthine, 2-ketoisocaproic acid, glutamic acid and aspartic acid, in a serum sample as combined markers in preparation of a kit for distinguishing patients with esophageal squamous carcinoma and esophageal epithelial atypical hyperplasia in subjects. The invention further relates to a kit for detecting patients with esophageal squamous cell carcinoma in subjects. The relative concentration of the combined markers in serum samples from the subjects is detected, the variables P of the combined marker are calculated based on a linear regression equation, and whether the subjects suffer from esophageal squamous cell carcinoma or not is judged based on a determined threshold value. The kit can realize high-sensitivity and high-efficiency detection of several metabolites involved in the invention, and has the advantages of low detection cost and good repeatability. The kit can be applied to clinical auxiliary diagnosis of the esophageal squamous cell carcinoma, has the advantages of high diagnosis sensitivity and capability of effectively distinguishing the esophageal squamous cell carcinoma from precancerous lesions, and has a relatively good application prospect.

Description

technical field [0001] The invention relates to the fields of analytical chemistry, biochemistry and clinical medicine. Background technique [0002] Esophageal cancer (esophageal cancer, EC) is a malignant tumor with a high incidence in my country. The annual incidence of cases in China accounts for about half of the total number of cases in the world. Esophageal squamous cell carcinoma (ESCC) is the most common esophageal cancer in my country. The overall 5-year survival rate after surgery is less than 25%. Esophageal squamous cell carcinoma has the characteristics of rapid progression, high degree of malignancy, and poor prognosis. The occurrence of esophageal squamous cell carcinoma is closely related to unhealthy eating habits such as eating pickled food and overheated food. The most common precancerous lesions of esophageal squamous cell carcinoma have similar clinical symptoms to those of esophageal squamous cell carcinoma. The distinction between the two is of great...

Claims

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8822G01N2030/8818
Inventor 陆欣李艳丽路鑫胡春秀王砚凤许国旺
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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