Use of metabolic biomarker in acute pancreatitis

A technology for acute pancreatitis and drugs, applied in the application field of metabolic biomarkers in acute pancreatitis, can solve the problems of lack of modern natural science support and verification of traditional Chinese medicine theory

Active Publication Date: 2019-06-21
PEOPLES HOSPITAL OF DEYANG CITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the application of fingerprint technology and mass spectrometry technology has promoted the discovery and identification of active ingredients in traditional Chinese medici...

Method used

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  • Use of metabolic biomarker in acute pancreatitis
  • Use of metabolic biomarker in acute pancreatitis
  • Use of metabolic biomarker in acute pancreatitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 Chinese medicine is to the treatment of acute pancreatitis

[0036] 1. Experimental materials

[0037] 1.1 Experimental main materials

[0038] Table 1 List of materials used

[0039]

[0040] 1.2 The main equipment of the experiment

[0041] Surgical instruments: 2 sterile microscopic curved forceps, 2 ophthalmic scissors, 1 needle forceps, and 1 scalpel.

[0042] Others: dry cotton balls, medical cotton swabs, sutures, surgical lights, surgical boards, fixed rubber bands, alcohol cotton, sterile gauze, sterile petri dishes, normal saline, 10mL syringes, 1mL syringes, No. 4 suture needles.

[0043] 2. Experimental method

[0044] 2.1 Animal grouping

[0045] 30 SPF-grade C57BL / 6 mice, male, 8 weeks old, weighing about 20g, were randomly divided into five groups after one week of adaptive feeding, with 6 mice in each group. The grouping conditions are shown in Table 2:

[0046] Table 2 Grouping of mice

[0047]

[0048] 2.2 Drug preparation

...

Embodiment 2

[0089] Example 2 Screening for metabolites related to acute pancreatitis

[0090] 1. Collection of samples

[0091] In this experiment, 10 mouse tissue samples were collected from each of the blank control group, the model group and the rhein+honokiol combined treatment group in the embodiment.

[0092] 2. Experimental information

[0093] 2.1 Instruments and reagents

[0094] Table 4 Reagents

[0095]

[0096] Table 5 Instruments

[0097]

[0098] 2.2 Experimental method

[0099] 2.2.1 Metabolite extraction

[0100] 1) Take 100mg of tissue and add 500μL of lysate (MeOH:H 2 O=1:1);

[0101] 2) Crushed in a tissue disruptor, 6,500MHz, 1min, oscillating 3 times;

[0102] 3) After vortex mixing, place in a -20°C refrigerator overnight;

[0103] 4) Centrifugation: 4°C, 13,000rpm, 20min;

[0104] 5) take the same volume of supernatant and freeze-dry;

[0105] 6) Add 100 μL of lysate to redissolve, and sonicate for 5 minutes;

[0106] 7) Centrifugation: 4°C, 13,000...

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Abstract

The invention discloses the use of a metabolic biomarker in acute pancreatitis. The invention is characterized by collecting normal acute pancreatitis and samples of drug therapy of the acute pancreatitis, performing metabolomics analysis, and discovering the metabolites which exhibit significant differences in different treatment groups, thereby using the metabolites to predict acute pancreatitis, and further achieving individualized treatment of patients.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to the application of metabolic biomarkers in acute pancreatitis. Background technique [0002] Acute pancreatitis (AP) refers to the activation of pancreatic enzymes caused by a variety of etiologies, followed by the local inflammation of the pancreas as the main feature. In severe cases, systemic inflammatory response syndrome (SIRS) may occur, and may be accompanied by organ dysfunction. Dysfunctional diseases. It is one of the common clinical acute abdomens, and its incidence has been increasing in recent years, with an overall fatality rate of 5%-10%. The conventional treatment methods are fasting, gastrointestinal decompression, inhibition of pancreatic juice secretion, anti-inflammation, and symptomatic and supportive treatment. At present, there are mainly the following types of Western medicines for the treatment of AP at home and abroad: somatostatin and its analogues (octreoti...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 麦刚刘航李雨黄承军
Owner PEOPLES HOSPITAL OF DEYANG CITY
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