The invention discloses a construction method of an
enzyme-reponsive multifunctional nano-
coating. The construction method comprises the following steps: firstly, immobilizing
avidin molecules to the surface of
dopamine-coated stainless steel; then, on the basis of specific recognition and binding effects between
avidin and
biotin, immobilizing biotinylated
heparin / PEI nanoparticles to the surface of a material, and further binding high-concentration
avidin molecules to residual
biotin on the surface of a
nanoparticle coating, so that a novel
biotin binding site is introduced; then, continuing to assemble biotinylated
enzyme-responsive polypeptide to the surfaces of the nanoparticles on the basis of an interaction between the biotin and the avidin; and finally, by virtue of an EDC / NHS / MES
coupling agent, immobilizing SDF-1[alpha] to an
amino terminal of the polypeptide in a covalent mode, so that the multifunctional nano-
coating, which has a
matrix metalloproteinase 9 responsive characteristic, is constructed. According to the construction method provided by the invention, by constructing a multifunctional layer, which has anti-coagulating and endothelial regeneration inducing capacities, on a
titanium surface,
blood compatibility and a damaged endothelial repair capacity of the material can be obviously improved.