Molecular marker for diagnosing or treating glioma and applications thereof
A molecular marker, glioma technology, applied in the fields of biomedicine and molecular biology, which can solve the problems of short curative effect and poor curative effect, etc.
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[0076] 1. Experimental materials
[0077] Human glioma cell lines U251, U87, and A172 were purchased from the Cell Bank of the Chinese Academy of Sciences. The grading standard was grade IV, and they grew adherently. DMEM complete medium was purchased from Gibco, cell culture flasks and culture plates were purchased from Corning, trypsin was purchased from Invitrogen, fetal bovine serum was purchased from Hyclone, double antibodies were purchased from Sigma, and PBS dry powder was purchased from Shanghai Gefan Biotech Co., Ltd. , Trizol was purchased from TAKARA Company, the whole gene expression profile chip and the whole microRNA expression chip were purchased by Shanghai Kangcheng Biological Co., Ltd. and entrusted for detection, the RT-qPCR kit was purchased from TAKARA Company, RIPA lysate, 5×SDS-PAGE loading buffer Solution, Tris-HCL matching gel kit and primary antibody diluent were purchased from Beyontian Company, protease inhibitors, Tris, SDS, glycine, Tween20, Lipo...
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