46 results about "Karyocytomegaly" patented technology

This invention concerns to a topical pharmaceutical composition comprising an water soluble Curcuma extract, and suitable excipients for said topical administration; the process for obtaining said pharmaceutical compositions; the use of different Curcuma extracts as photosensitizing agents for the treatment of proliferative diseases; and the use of Curcuma extract or curcuminoids in combination with a radiation for the treatment of proliferative diseases on eukaryote cells.

This invention concerns to a topical pharmaceutical composition comprising an water soluble Curcuma extract, and suitable excipients for said topical administration; the process for obtaining said pharmaceutical compositions; the use of different Curcuma extracts as photosensitizing agents for the treatment of proliferative diseases; and the use of Curcuma extract or curcuminoids in combination with a radiation for the treatment of proliferative diseases on eukaryote cells.

Aptamers bind to Listeria surface proteins. A method of assaying a sample for the presence of Listeria monocytogenes includes exposing the sample to an aptamer that specifically binds one of the following proteins: Listeria monocytogenes internalin A protein, Listeria monocytogenes internalin E protein, and Listeria monocytogenes 0610 protein. The presence of Listeria monocytogenes in the sample is detected when the aptamer binds the protein present in the sample. A method of treating Listeria monocytogenes infection includes administering the aptamers to the mammal at a concentration sufficient to reduce Listeria monocytogenes infection.

The invention discloses a nucleic acid aptamer capable of specifically recognizing Listeria monocytogenes, a screening method and application thereof. By using the SELEX (Systematic Evolution of Ligands By Exponential Enrichment) technology, a single-chain DNA oligonucleotide aptamer capable of specifically recognizing Listeria monocytogenes is obtained, and the aptamer can be transformed into a reporting aptamer by means of fluorescein labels and the like to be used for detecting Listeria monocytogenes. The sequence of the aptamer has wide application prospects in the aspect of accurately and fast detecting Listeria monocytogenes in food.

The present invention is directed to satisfying the need to detect microbial contamination of food products. The described bioseparator / bioreactor coupled with an optical / electrochemical biosensor was able to specifically detect E. coli O157:H7 from 8.8×101 to 8.8×106 CFU / ml in 2.5 hours without any enrichment. Using this invention, concentrations of S. Typhimurium ranging from 8.6×102 to 8.6×106 CFU / ml in pure culture were detected in 2 hours without any enrichment. The invention may also be used for the detection of S. Seftenberg, which has the same sensitivity as S. Typhimurium. Other pathogens such as L. monocytogenes and S. Heidleberg did not interfere with the detection. The optimum inner diameter of the 25 cm long column for the detection of E. coli O157:H7 is 250 μm. The detection limit for other microbial pathogens may be controlled by changing the length of capillary columns, using higher concentration of the labeled antibodies, altering the flow rate and concentration of the substrate, and increasing the reaction temperature to 37° C.

The present invention is directed to isolated Listeria monocytogenes bacteriophage, and methods of using Listeria monocytogenes bacteriophage, or polynucleotides and polypeptides derived therefrom, to control the growth or contamination of food products by Listeria monocytogenes. The present invention also contemplates the use of Listeria monocytogenes bacteriophage, and polynucleotides and polypeptides derived therefrom, for the treatment of host infections or environmental contamination by Listeria monocytogenes.

The invention is directed to a fermented and clarified nisin-containing whey and a method of making that can be used to produce a stabilized food product by adding, for example, to the pack water of fresh mozzarella cheese. The resulting stabilized food product retards or limits below detection levels the growth of toxins from pathogenic bacterial contaminants when the nisin-containing whey is added in amounts between about 10 to about 30% to the food product. The stabilized food product improves the safety of the food by retarding the growth of Listeria monocytogenes and improves the shelf life of the product by retarding the growth of gas forming bacteria such as bacteria from the Leuconostoc species.

The present invention is directed to isolated Listeria monocytogenes bacteriophage, and methods of using Listeria monocytogenes bacteriophage, or polynucleotides and polypeptides derived therefrom, to control the growth or contamination of food products by Listeria monocytogenes. The present invention also contemplates the use of Listeria monocytogenes bacteriophage, and polynucleotides and polypeptides derived therefrom, for the treatment of host infections or environmental contamination by Listeria monocytogenes.

The invention belongs to the technical field of biological medicines, and particularly relates to a multi-drug-carrying targeted nanoparticle and a preparation method and application thereof. The multi-drug-carrying targeted nanoparticle is prepared by loading a polyphenol compound, iRhom2 siRNA and TNF-Alpha inhibitor on a gold-coated iron oxide core-shell nanoparticle (GNPs), and the molar ratioof the gold-coated iron oxide core-shell nanoparticle, the polyphenol compound, iRhom2 siRNA and the TNF-Alpha inhibitor is 1:(1 to 3):(1 to 3):(1 to 3). The multi-drug-carrying targeted nanoparticledisclosed by the invention increases the bioactivity and bioavailability of the polyphenol compound, and can effectively prevent and treat related diseases as the result of septicemia caused by listeria monocytogenes.

The present invention relates to virulent (lytic) Listeria monocytogenes phage from the Myoviridae family, preferably P100, alone or in combination with other virulent phages. P100 and the endolysin from P100 can be administered to food products, to the components that will be added to food products, and / or to the infrastructure of the food processing plants within which such food products are processed, or the containers or wraps in which such foods are stored and / or shipped, in order to reduce Listeria monocytogenes contamination. P100 can also be used in the present invention to identify Listeria monocytogenes bacteria present on (or within) foodstuffs, as well as those Listeria monocytogenes bacteria present in the equipment or the general environment of the food processing plants in which the foodstuffs are being processed and in animals infected with Listeria monocytogenes. The phage and the endolysin of the present invention can also be used to treat animals infected with Listeria monocytogenes. P100 will kill the bacteria that are within its host range with great efficiency and will propagate to high titer thereon. P100 can be combined with other lytic phage, and / or with other antimicrobial agents to reduce or eliminate Listeria.

The invention provides a multiple PCR detection kit for Listeria monocytogenes and Listeria illichii. The kit comprises a gene lmo0601 detection primer and a gene smcl detection primer. The inventionestablishes a multiple PCR method for rapidly detecting Listeria monocytogenes and Listeria illichii by using the two pairs of primers, and the method has the advantages of good specificity, high sensitivity, high detection speed, easy operation and simple result judgment.

The invention provides a real-time fluorescent quantitative helicase-dependent isothermal nucleic acid amplification method for detecting listeria monocytogenes. Primer pair sequences having specific amplification function on listeria monocytogenes are designed so as to reflect a natural process for simulating the replication of in-vivo DNA at a constant temperature, DNA double strands are solved at a constant temperature by virtue of helicase, the listeria monocytogenes genomic DNA is adopted as a template, specific isothermal nucleic acid amplification is performed so as to achieve the exponential growth of the target sequence, the real-time fluorescent helicase-dependent isothermal nucleic acid amplification detection method is established, detection results are analyzed by virtue of an isothermal amplification curve and melting curve of the genomic DNA so that listeria monocytogenes is rapidly detected. By the method, the disadvantage that the traditional PCR needs to rely on the repeated heating and cooling of an instrument to obtain the single-stranded template is overcome and the method is simple in principle, convenient to operate and listeria monocytogenes in foods can be rapidly, simply, sensitively and specifically detected.

Embodiments of the disclosure relate to isolated nucleic acid sequences, methods of use thereof, and workflows for detection of several Listeria species in a sample, particularly in a food or environmental sample. Embodiments of the disclosure may also be used to detect one or more species or strains of Listeria from each other, for example L. grayi may be detected independently of other Listeria spp. Some embodiments also describe a duplexed assay that can detect L. monocytogenes, L. innocua, L. welshimeri, L. seelgeri, L. marthii (formerly incertae-sedis), L. ivanovii, and L. grayi. Kits for detection of Listeria are also described. In some embodiments, methods and kits of the disclosure may comprise a TAQMAN® assay. In some embodiments, 0.2-2 cfu of Listeria spp. are detected using the compositions, methods and kits after a 24-28 hour enrichment period.