The present invention relates to a universal cell therapy product for expressing antigen recognition regions, and a preparation method and an application thereof. The antigen recognition regions aiming at tumor cell antigens are carried on universal cells without an HLA restriction, the antigens are degraded after treatment, and tumor cells are changed in series until death, thereby playing a rolein treatment. A provided new strategy for treating tumors is expected to replace individualized CART, remarkably reduces preparation costs of CART cells, achieves an aim of treating the tumors, doesnot generate cytokine storm and neurotoxicity, reduces threshold of CART treatment, and benefits more patients.