52 results about "Genomic technology" patented technology

Methods and compositions for determining a methylated cytosine profile of a target nucleic acid sequence are provided.

The invention relates to a pathogenic microorganism genome database and an establishment method thereof, and belongs to the technical field of meta-genomes. The method comprises the following steps ofdata acquisition, wherein pathogenic microorganism genome data is obtained; strain genome screening, wherein species strain genomes are selected according to a predetermined screening rule; plasmid sequence removal, wherein plasmid sequences existing in the strain genomes obtained in the last step are removed; filtration, wherein according to a predetermined filtering rule, strains with incorrectlabeling information, incomplete chromosome assembly and incorrect classification are removed to obtain a reference strain genome of the species; fusion genome construction, wherein the reference strain genome is interrupted, redundancy is removed, reassembly is performed, and the sequences are reassembled to obtain a fusion genome of the species; database assembly, wherein the above steps are repeated to obtain the fusion genome of the predetermined species, summary is performed, and the pathogenic microorganism genome database is obtained. The genome database has the advantages of not onlyhaving a high precision rate, but also having short analysis time and reducing the cost.

Embodiments relate to genomic technologies using adaptive spline analysis that predict responses of cancer cells. For example, responses of cancer cells to specific medications and / or treatments may be predicted based on adaptive linear spline analyses.

The invention relates to a method for integration of commercial breeding of plants and genomic methodology comprising the steps of: a) plant population development by crossing a Parent 1 and a Parent 2 to generate a Population I; b) crossing Parent 1 with individuals from Population I to generated a Population II; c) crossing Parent 1 with individuals from population II to generate a Population III; d) randomly selecting at least one plant per each line in Population III and collecting genetic material from the random plant; e) self pollinating selected plants from population III to generate a Population IV; f) evaluating and selecting plants of Population IV; and g) using selecting progeny plants of Population IV in test crosses for evaluating the potential to develop new commercial cultivars; where the genetic material in step d) is used to develop marker profiles of each plant to map QTL and major gene loci as part of the evaluation of plants in step f).

The invention discloses novel feruloyl esterase, a mutant and an application thereof, and belongs to the field of bioengineering. The method comprises the following steps: firstly, successfully screening out a feruloyl esterase gene from anaerobic fungi Neocallimatix sp. by utilizing a genomics technology; according to the preference of a prokaryotic system codon, the GC content of a feruloyl esterase gene and the secondary structure of mRNA, and codon optimization is carried out on the feruloyl esterase gene to obtain a novel feruloyl esterase gene sequence. The enzyme has an esterase regionand a glucoside hydrolase 11 region, site-specific mutagenesis is carried out on the two regions; the obtained mutant gene is also expressed in escherichia coli; separation and purification results show that the novel feruloyl esterase and the mutant thereof are successfully expressed in escherichia coli, and the esterase activity of the mutant is far lower than that of a non-mutant, so that the strain source of the feruloyl esterase is enriched, and the feruloyl esterase has theoretical guiding significance in the aspects of improving the digestibility of feed fibers and improving the production performance of animals.

The invention discloses an alkaline cellulase gene and application thereof in the field of textiles or detergents. The nucleotide sequence is disclosed as SEQ ID NO:1, and the coding amino acid sequence is disclosed as SEQ ID NO:2. The gene is a new DNA sequence which is obtained from soil metagenome by a metagenome technique and has functions which are not researched. The researches on the structure and functions of the protein coded by the DNA sequence prove that the recombinant protein expressed by the DNA sequence has favorable alkali-resistant cellulose enzyme activity, and part of metal ions and surfactants can obviously enhance the activity of the recombinant protein decomposed cellulose substrate. The expression product of the DNA sequence can be used as a new enzyme source in textile or detergent industry, and has wide industrial application prospects.

An agriculture management and analysis system for analysis, interpretation, and visualization of genetic data to improve production, performance, and management of agriculture, such as livestock.

The invention relates to novel metal ion tolerance keratinase and application thereof, and provides keratinase based on metagenome technology excavation and an application method of the keratinase, belonging to the field of industrial biotechnology. The overall length of the keratinase is 1,149bp, and 382 amino acids are encoded; take B.subtilis WB600 as an example, the heterologous expression ofa keratinase gene is successfully realized. The method for obtaining the keratinase gene is convenient and feasible, the gene is subjected to recombined expression, a constructed recombination straincan realize secretory expression of recombined keratinase, the recombined keratinase has relatively good tolerance to metal ions and a surface active agent, and the stability of enzyme is relatively good. Recombinant bacteria are short in fermentation period and suitable for industrial production in large scale. In addition, the enzyme has good reducing capacity and can be applied to research in which a biological method is used for preparing nano-silver particles, and the prepared nano-silver particles have good forms and relatively good bacteriostatic activity.

The invention discloses a sealed poultry and livestock house internal environment microbe aerosol monitoring method, which comprises the following steps that (1) sample points are arranged: point distribution is performed in a poultry and livestock house by a quartering diagonal line point selection method; at least five sampling points are set; (2) the continuous sampling is performed on the sampling points in the poultry and livestock house by an air microbe aerosol sampling device; a metagenome technology is used for analyzing the microbe kinds and contents; the bioinformatics is combined for obtaining information of dominant bacteria, pathogenic bacteria and potential pathogenic bacteria, wherein the continuous sampling process comprises the sampling during the behaviors of feed feeding, feed taking peak period, poultry and livestock resting, poultry and livestock free activity, poultry and livestock house cleaning and pad material replacement.

The invention discloses a method for rapid and accurate selective breeding of three-line rice sterile lines by a rice genomics technology. By assisted screening combination of conventional breeding with molecular markers, the method can effectively eliminate fertility restoring genes, and genetic background screening is performed by a genotyping technique based on rice whole genome sequencing, andsterile lines and corresponding maintainer lines having genetic background closer to target parents can be screened. Compared with the prior art, the method greatly shortens improved generations of the maintainer lines and backcross generations of the sterile lines, and the three-line rice sterile lines with excellent comprehensive characters can be accurately selectively bred within only 4-5 years.

The invention discloses a method for quickly and accurately selecting a three-line rice sterile line by using rice genomics technology. The method combines conventional breeding with molecular marker-assisted screening to effectively eliminate fertility restoration genes, and at the same time utilizes the method based on the whole genome of rice Genetic background screening by sequencing genotyping technology can screen and obtain sterile lines and corresponding maintainers whose genetic background is closer to the target parent. Compared with the existing technology, this method greatly shortens the improved generation of the maintainer line and the backcross generation of the sterile line, and can accurately breed a three-line rice sterile line with excellent comprehensive characteristics in only 4-5 years.

The invention belongs to the technical field of landscape genomics, and particularly relates to a method for evaluating species adaptation potential by landscape genomics. The method comprises the following steps that: carrying out sample collection; extracting the DNA (DeoxyriboNucleic Acid) of a sample in S1, and carrying out genetic typing detection on all sample individuals to obtain the original data of the genetic typing detection; and obtaining the optimal value of K which can most favorably represent a major group structure, carrying out exceptional locus detection to obtain the data matrix of a gene exceptional locus, and carrying out adaptive gene locus detection to obtain adaptive gene locus data matrix. According to adaptive gene locus data and non-adaptive gene locus data, a proper landscape adaptation index is established to measure the species adaptation potential, wherein the index has an important reference value for introduction and domestication in garden plant cultivation.

The invention discloses a method for eliminating three-dimensional genomics technology noise by utilizing exonuclease combination. The method comprises the step of adding exonuclease combination to eliminate adjacently connected linear DNA when performing the adjacent connection at the key step of the three-dimensional genomics technology, thereby improving the cyclic effective interaction proportion. The excellent performance of the exonuclease combination for eliminating the linear DNA provides an effective measure for eliminating the noise through the enzyme digestion of the exonuclease combination after the C-technology connection; the method for eliminating the three-dimensional genomics technology noise by utilizing exonuclease combination has the advantages of being convenient, fastand efficient, the possibility of producing the false-positive product or signal can be reduced, the effective interaction cyclic DNA proportion is enhanced, the terminal effective interaction data output ratio is greatly improved, the sequencing space is saved, the sequencing cost is reduced, and the operation for eliminating the resource consumed by ineffective information in the big data by using the complex biological information at later is simplified.

The invention relates to a method for breeding a new rice variety with a wild rice genetic background. The method utilizes a rice genomics technology to rapidly and accurately breed the new rice variety with the wild rice genetic background. More importantly, the inventor of the invention improves screening conditions in the breeding process, so that the technical difficulty and the bottleneck in the crossbreeding process of the wild rice and cultivated rice are overcome, the new rice variety with the wild rice genetic background can be bred, a cultivated rice genetic background is effectively expanded, the serious problem of existing variety homogenization is solved, novel germplasm resources can be created favorably, the yield of hybrid rice is increased, and breakthrough varieties are obtained.

The invention relates to a comparative analysis method for ancient chromosome evolution of sisoridae fishes. According to the invention, a comparative genomic technology is utilized to analyze the ancient chromosomes of the sisoridae fishes, and evolution research on the chromosomes of the sisoridae fishes is beneficial for exploring the evolution rule of the genomes of the sisoridae fishes and provides basic data for the evolution research and resource protection of the sisoridae fishes. The method provided by the invention comprises the steps of construction of the chromosome sequence of glyptosternon maculatum, phylogenetic analysis, comparison with the chromosomes of sibling species, ancient chromosome construction and the like. The method is suitable for the sisoridae fishes in Tibetan, and can be widely applied to resource investigation and protection research of the sisoridae fishes in the Qinghai-Tibet Plateau.

The invention relates to an SNP molecular marker for identifying the producing place of radix scutellariae and a method and application thereof. The molecular marker comprises 20 SNP molecular markers, and the nucleotide sequences of the 20 SNP molecular markers are shown as SEQ ID NO.1 to SEQ ID NO.20. According to the SNP molecular marker for identifying the production place of the radix scutellariae, which is disclosed by the invention, the SNP marker is obtained on the genome level through a genomics technology, the radix scutellariae of which the production places are Shaanxi, Shanxi or Shandong can be rapidly and accurately identified by adopting the SNP molecular marker disclosed by the invention, the radix scutellariae with different production places is screened, the identification accuracy is high, the stability is high, and the SNP molecular marker is easy to popularize and apply in a wide range.

The invention discloses a method for selecting and breeding a three-line rice restoring line by a rice genomic technology rapidly and accurately. According to the method, the conventional breeding iscombined with molecular marker auxiliary screening, genetic background screening is conducted by a genotyping technology based on rice whole genome sequencing, and the restoring line which contains ahomozygous sterility restoring gene and has the genetic background closer to a target parent can be screened and obtained. Compared with the prior art, the method has the following advantages: the improved generation of the restoring line is greatly shortened, and the three-line rice restoring line with excellent comprehensive characters can be selected and bred accurately for only 3 to 4 years.

The invention relates to a SNP molecular marker for identifying scutellaria baicalensis produced in Inner Mongolia as well as a method and application of the SNP molecular marker. The molecular markercomprises 19 SNP molecular marker bodies, and the nucleotide sequences of the 19 SNP molecular marker bodies are shown as SEQ ID NO.1 - SEQ ID NO.19. According to the SNP molecular marker for identifying the scutellaria baicalensis produced in the Inner Mongolia as well as the method and the application of the SNP molecular marker, the genomics technology is adopted, the SNP marker is obtained onthe genome level of the scutellaria baicalensis, and whether a detected scutellaria baicalensis is an Inner Mongolia producing area or not can be rapidly and accurately determined.

The present invention relates to the application of genomics technology to generate nutritional compounds in algae, such as microalgae. The present invention discloses a novel method to modify algae to produce a variety of nutritional compounds. Specifically, this method utilizes microalgae genomics technology to manipulate the existing metabolic pathways in the genomes of microalgae and therefore induce the algae to modify secondary metabolic pathways to increase the level of existing nutritional compounds or produce novel nutritional compounds with a variety of commercial applications for improving human and animal nutrition and health.

The invention discloses a method for quickly and accurately breeding a maize inbred line by a maize whole genomics technology, wherein the method combines the traditional breeding with a whole genomesequencing technology. Through field agronomic characteristic screening and genotype analyzing, an excellent individual plant with excellent trait genes of both parents and genetic background more close to a target parent is screened, finally the stable inbred line combined with excellent traits of both parents are bred through continuous selfing. Compared with the prior art, the method performs genetic background screening through a genetic typing technology of conventional selection and whole genome sequencing, the improvement generation of the inbred line is greatly shortened, the maize inbred line with excellent comprehensive characters can be accurately bred within only 2 to 3 years.

The invention relates to novel metal ion tolerance keratinase and application thereof, and provides keratinase based on metagenome technology excavation and an application method of the keratinase, belonging to the field of industrial biotechnology. The overall length of the keratinase is 1,149bp, and 382 amino acids are encoded; take B.subtilis WB600 as an example, the heterologous expression ofa keratinase gene is successfully realized. The method for obtaining the keratinase gene is convenient and feasible, the gene is subjected to recombined expression, a constructed recombination straincan realize secretory expression of recombined keratinase, the recombined keratinase has relatively good tolerance to metal ions and a surface active agent, and the stability of enzyme is relatively good. Recombinant bacteria are short in fermentation period and suitable for industrial production in large scale. In addition, the enzyme has good reducing capacity and can be applied to research in which a biological method is used for preparing nano-silver particles, and the prepared nano-silver particles have good forms and relatively good bacteriostatic activity.

The invention provides a method or evaluating existence of antibiotics in a meat product. Firstly, an in-vitro chemostat model of human intestinal flora is constructed, and the to-be-evaluated meat product is added into the model; and the intestinal flora conditions of the in-vitro chemostat model, after and before the meat product is added, are obtained through a 16S rDNA sequencing method, and if the intestinal flora has no fluctuation change, the meat product does not contain the antibiotics, and otherwise, the meat product contains the antibiotics. The evaluating method combines the in-vitro chemostat model and the metagenome technology, is not limited by the variety of the antibiotics, improves the reliability, the accuracy and the sensitivity of detection of vitamin residues, and provides an excellent model and an advanced detecting technique for the establishment of a more scientific and comprehensive antibiotics residue safety assessment system for antibiotics-free products.

The invention discloses a susceptible gene for acute exacerbation of chronic obstructive pulmonary disease and application of the susceptible gene in prediction of susceptible acute exacerbation of chronic obstructive pulmonary disease. Clinical specimens such as blood of a patient in the chronic obstructive pulmonary disease acute exacerbation stage are collected, blood circulation DNA whole exons of the COPD patient are detected through a genomics technology, and 20 AECOPD specific susceptibility genes are obtained by comparing the gene difference of the whole exons of the chronic obstructive pulmonary disease patient in the stable stage and the acute exacerbation stage; through gene detection of a verification queue, a highly specific gene for predicting acute exacerbation of chronic obstructive pulmonary disease is identified in the 20 genes, and can be used for screening susceptible patients with acute exacerbation of chronic obstructive pulmonary disease, so that a basis is provided for researching and verifying prognosis and recurrence markers of susceptible acute exacerbation of chronic obstructive pulmonary disease in a larger clinical sample in the future; wide application prospects are realized.

The invention discloses genes and proteins for prosthetic group-free chloroperoxidase CPO822 with multiple functions, particularly capability of degrading benzopyrene and having activities of peroxidase, catalase, halogenase and esterase, and application of the genes and proteins. The invention has the beneficial effects that by virtue of a metagenome technology, a novel DNA sequence without functional study is obtained from soil metagenome; study results of the structures and functions of proteins coded by the DNA sequence show that a recombinant protein expressed by the DNA sequence has polyfunctionality, has a wider catalytic reaction range than existing separated peroxidase, can serve as a novel enzyme source to be applied to the fields such as washing, wastewater treatment, disinfection, lignin removal, production of epoxy derivatives, degradation of benzopyrene and the like, and has wide industrial application prospects. In addition, the study discovers that the enzyme is stableunder a wide-range pH value, is high in stability at a high temperature, and has great significances on wide popularization and application.

The invention belongs to the field of water ecological safety, and particularly relates to a method for identifying pathogenic bacteria carrying toxic factors in a water body based on a metagenome technology. According to the method, through a metagenome sequencing technology and a gene assembling method, the types and the number of the toxic factors in a water body are determined on the gene level, and pathogen host information of the toxic factors is identified, so that pathogenic bacteria carrying the toxic factors in the water body and potential health risks of the pathogenic bacteria aredetermined. The method has the characteristics of high flux, quickness, simplicity and convenience, and is suitable for large-scale sampling investigation and research.

The invention provides SSR (simple sequence repeat) molecular marker primers and method for identifying scutellaria baicalensis in the northeast region, and the method comprises the following steps: taking total DNA (deoxyribonucleic acid) of a scutellaria baicalensis sample to be identified as a template, and performing PCR (polymerase chain reaction) amplification by using the SSR molecular marker primers to obtain an amplification product; performing capillary electrophoresis sequencing typing on the amplification product to obtain a typing result of the amplification product; carrying out peak map interpretation on the typing result of the amplification product by using GeneMarker 4.0 to obtain an allele matrix; according to the allele matrix, carrying out GenALEx calculation to obtain genetic diversity data of the scutellaria baicalensis; and on the basis of genetic diversity data obtained by GenALEx calculation, carrying out UPGMA clustering analysis through MEGA to construct a clustering tree. A genome technology and an SSR molecular marker technology are applied, the SSR primers are designed by utilizing the scutellaria baicalensis genome, the SSR primers are screened through scutellaria baicalensis samples in Hebei, Shanxi, Shandong, Shaanxi, Inner Mongolia, Liaoning, Jilin and the like, and the primers are used for specifically identifying scutellaria baicalensis samples in the northeast region, are high in accuracy and stability and are easy to popularize and apply in a large scale.

The invention relates to a sample treatment method for ruminant rumen microorganism research, which comprises a sample selection method and a sample treatment method and process, the sample selection mode is divided into four modes, and the four modes are respectively a solid-liquid mixed sample, a solid-liquid simultaneous selection, only a solid phase sample and only a liquid phase sample. The sample treatment method and process comprise three steps of sampling time selection, sample collection and sample treatment, and the sample treatment comprises liquid phase sample treatment and solid phase sample treatment. The sample treatment method for ruminant rumen microorganism research has the beneficial effects that rumen microorganism research is carried out by adopting a solid-liquid separation method, deviation generated by selecting rumen fluid for research can be effectively avoided, meanwhile, target species can be more comprehensively screened out, and the sample treatment method is simple and convenient to operate. And the problem of hybrid genome pollution generated when a metagenome technology is used for research can be improved to a great extent.