The invention relates to a negative pressure mask for endoscopic examination and an adsorption filtering device, and belongs to the technical field of medical instruments. The negative pressure mask comprises a mask body, an endoscope socket, a one-way air inlet valve, an oxygen interface, an exhalation airflow discharge hole and a negative pressure filtering and pressure regulating device, wherein the mask body is provided with the one-way air inlet valve and the oxygen interface; the endoscope socket and the exhalation airflow discharge hole are arranged at the mouth and nose part of the mask body; and the discharge hole is connected with an activated carbon adsorption column and the negative pressure filtering and pressure regulating device through conduits. The negative pressure mask has a reasonable structure, is simple to manufacture, can effectively prevent spread of pathogens carried by exhaled gas, droplets and aerosol of an examinee, prevents infection of an operator and potion to the surrounding environment, and is suitable for endoscopic examination of high-infectious respiratory system diseases. The oxygen supply conduit ensures oxygen supply of patients, and meanwhile, pressure of the negative-pressure suction can be regulated and controlled by adjusting the height of the water surface in a pressure regulating cavity, so that the pressure of the mask is maintainedin a safe range, influence on the breathing comfort of the patient is avoided, increasing of work of breathing is avoided, and safety of the patient is ensured.