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Application of circ_slc7a5 molecular markers in blood in the diagnosis and prognosis of esophageal squamous cell carcinoma

A molecular marker and technology for esophageal squamous cell carcinoma, applied in the field of tumor molecular biology, can solve the problems of poor compliance in endoscopic screening for esophageal cancer, and achieve the effects of avoiding excessive medical treatment, relieving pain, and saving medical resources

Active Publication Date: 2019-10-18
江苏万成生物医学研究院有限公司
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  • Abstract
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  • Claims
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Problems solved by technology

[0005] In order to solve the above problems, the present invention discloses the application of circ_SLC7A5 molecular marker in blood in the diagnosis and prognosis of esophageal squamous cell carcinoma, aiming at the poor compliance of the target population with endoscopic screening for esophageal cancer and clinically diagnosed patients with esophageal squamous cell carcinoma Most of them are in the middle and advanced stages. It is proposed to find the ideal molecular early warning signal in the process of esophageal squamous cell carcinoma, and carry out targeted endoscopic examination and pathological diagnosis, so as to alleviate the suffering of patients, avoid excessive medical treatment, and save medical resources.

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  • Application of circ_slc7a5 molecular markers in blood in the diagnosis and prognosis of esophageal squamous cell carcinoma
  • Application of circ_slc7a5 molecular markers in blood in the diagnosis and prognosis of esophageal squamous cell carcinoma
  • Application of circ_slc7a5 molecular markers in blood in the diagnosis and prognosis of esophageal squamous cell carcinoma

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Embodiment Construction

[0032] The present invention will be further explained below in conjunction with the accompanying drawings and specific embodiments. It should be understood that the following specific embodiments are only used to illustrate the present invention and are not intended to limit the scope of the present invention.

[0033] Such as Figure 1 to Figure 8 As shown, the present invention discloses the application of a circ_SLC7A5 molecular marker in blood in the diagnosis and prognosis of esophageal squamous cell carcinoma. The nucleotide sequence of the circ_SLC7A5 molecular marker is SEQ ID NO.1.

[0034] 1. RNA Sanger Sequencing

[0035] (1) The amplified products were inserted into T-vectors for Sanger sequencing to determine their full lengths. Different primers were designed to confirm the splicing back junction of circ_SLC7A5. Primers were synthesized by Invitrogen Company (Shanghai, China), and Sanger sequencing was performed by Realgene Company in Nanjing, China. Test res...

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Abstract

The invention discloses application of a circ_SLC7A5 molecular marker in blood in diagnosing esophageal squamous carcinoma and prognosis, and relates to discovery, detection and application of the circ_SLC7A5 molecular marker. A specific detection primer for real-time quantitative PRC is designed and synthesized. Circ_SLC7A5 in the blood can serve as a plasma marker for ESCC patients, aiming at the status quo that a target group of people have poor compliance on esophagus cancer endoscope screening, and most of esophageal squamous carcinoma patients with definite diagnosis in clinic are in themiddle and advanced stage, ideal molecular early-warning signals are found out in the esophagus squamous cell canceration process, endoscopy is targetedly carried out, the pathology is conformed, sothat the pain of the patients is alleviated, excessive medical treatment is avoided, and medical resources are saved.

Description

technical field [0001] The invention relates to the field of tumor molecular biology, in particular to the application of a circ_SLC7A5 molecular marker in blood in the diagnosis and prognosis of esophageal squamous cell carcinoma. Background technique [0002] Esophageal cancer is one of the most common malignant tumors that seriously threaten human health. There are two main pathological types: squamous cell carcinoma and adenocarcinoma. 90% of esophageal cancers are squamous cell carcinomas, and their distribution has obvious geographical differences. The incidence of ESCC in my country accounts for more than 70% of the world, and the prognosis is poor. The 5-year overall survival rate is only 15%-25%. In 2016, the Journal of Clinician Cancer reported that esophageal cancer has become one of the five most common tumors in China, ranking third among men and fifth among women. [0003] Circular RNAs (circRNAs), a new type of endogenous non-coding RNA, were identified as t...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/11
Inventor 康宁朱桂芳曹秀峰
Owner 江苏万成生物医学研究院有限公司
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