Application of circ_slc7a5 molecular markers in blood in the diagnosis and prognosis of esophageal squamous cell carcinoma
A molecular marker and technology for esophageal squamous cell carcinoma, applied in the field of tumor molecular biology, can solve the problems of poor compliance in endoscopic screening for esophageal cancer, and achieve the effects of avoiding excessive medical treatment, relieving pain, and saving medical resources
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[0032] The present invention will be further explained below in conjunction with the accompanying drawings and specific embodiments. It should be understood that the following specific embodiments are only used to illustrate the present invention and are not intended to limit the scope of the present invention.
[0033] Such as Figure 1 to Figure 8 As shown, the present invention discloses the application of a circ_SLC7A5 molecular marker in blood in the diagnosis and prognosis of esophageal squamous cell carcinoma. The nucleotide sequence of the circ_SLC7A5 molecular marker is SEQ ID NO.1.
[0034] 1. RNA Sanger Sequencing
[0035] (1) The amplified products were inserted into T-vectors for Sanger sequencing to determine their full lengths. Different primers were designed to confirm the splicing back junction of circ_SLC7A5. Primers were synthesized by Invitrogen Company (Shanghai, China), and Sanger sequencing was performed by Realgene Company in Nanjing, China. Test res...
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