This invention is directed to an optically-based method and
system for
analyte detection using
solid phase immobilization, specific
analyte labels adapted for
signal generation and corresponding processes for the utilization thereof. The enumeration detection method disclosed herein narrows the area for
signal observation, thus, improving detectable
signal to background ratio. The
system is comprised of a platform / support for immobilizing a sample stage having a labeled sample (
analyte complex) bound thereto, a
radiation source, an optical apparatus for generating and directing
radiation at said sample and a control that obtains data and then conducts analyses using
digital image data. Upon engagement of the
system, the sample generates a signal capable of differentiation from background signal, both of which are collected and imaged with a
signal detector that generated a
sample image to a
data processing apparatus. This apparatus receives signal measurements and, in turn, enumerates individual binding events. Generated signal may be increased via selected
mass enhancement. The invention, enumeration
assay methodology detecting individual binding events, may be used, for example, in analyses to detect analyte or confirm results in both research, commercial and
point of care applications.