The present invention relates generally to the field of
cell culture, which is a laboratory process used primarily for the growth, propagation, and production of cells for analysis and the production and harvesting of
cell products. The present invention comprises functionalized and / or engineered hydrogel microcarriers that exhibit any or all of the following properties: controllable
buoyancy, ferro- or
paramagnetism, molecular or fabricated reporting elements, and optical
clarity. The microcarriers are used in a
bioreactor that employs external forces to control said
microcarrier kinetic energy and translational or positional orientation in order to facilitate
cell growth and / or cellular analysis. The
bioreactor can be part of an automated
system that employs any or all of the following; a
microcarrier manufacturing method, a monitoring method, a
cell culture method, and an analytical method. Either a single
bioreactor or a plurality of bioreactors are used in the automated
system to enable
cell culture and analysis with a minimum of human intervention.