The invention discloses a partial base deletion
muscle inhibin
gene capable of being expressed in a mouse and application. An MSTN
gene comprises two introns and three
exon subsequence. 6 bases are deleted at the 175th-180th bit of the third
exon are deleted, a
cutting carrier containing partial base deletion
muscle inhibin genes is constructed, a knock-out mouse containing the partial base deletion
muscle inhibin genes is prepared, the
cutting carrier containing the partial base deletion muscle inhibin genes is applied to MSTN
organism character researches, the muscle amount of mouse
skeletal muscle is increased, a double muscling phenomenon is generated, and the mouse
basal metabolic rate and mouse body temperature are reduced. Through a
genetic engineering method, base knock-out is performed on the original MSTN
gene of the mouse, normal physiological functions of the
organism are not affected while the muscle amount of the mouse
skeletal muscle is increased, the muscle amount of the mouse can be quickly improved, and the partial base deletion muscle inhibin gene has large application prospect.