The invention discloses an array substrate, a manufacturing method of the array substrate and a display panel and relates to the technical field of display. The array substrate comprises a flat layer, a first electrode layer, an insulating layer and a second electrode layer which are sequentially located above a substrate body. The second electrode layer comprises a plurality of electrode bars, and the flat layer comprises a plurality of protrusions protruding towards the second electrode layer. At least part of the first electrode layer is formed on the protrusions. Preferably, the positions, corresponding to the gaps between the adjacent electrode bars, of the flat layer are designed with protrusions. According to the array substrate, the manufacturing method of the array substrate and the display panel, due to the protrusions designed on the flat layer, the vertical distance between electrodes of the first electrode layer and the second electrode layer is reduced, the effects of improving the horizontal electric field intensity and relieving the perpendicular electric field intensity distribution are achieved, and therefore the display panel can have high brightness under the low driving voltage.