The present invention provides a kind of method of
tissue culture rapid breeding Chinese fir, relates to the technical field of
plant breeding and propagation, the method of
tissue culture rapid breeding Chinese fir of the present invention comprises the following steps: (1) the preparation of explant, (2) the preparation of explant Pretreatment, (3) cultivation of culture medium, (4)
transplanting of rooted seedlings; the method for rapid breeding of Chinese fir by
tissue culture of the present invention induces the
callus of Chinese fir on MS medium containing different
hormone ratios, through induction culture culture medium, proliferation medium and rooting medium, each medium provides the factors needed for explants during rooting and strong seedlings, can provide good conditions for rooting, promote healthy and strong seedlings, and obtain regenerated seedlings of Chinese fir. The invention has the advantages of convenient material collection, low cost, simple operation, fast multiplication speed, high
reproduction coefficient, good economic and ecological benefits, and is suitable for large-scale production.