30results about How to "Improve ethanol yield" patented technology

The invention relates to a method for producing ethanol by continuous enzymolysis and fermentation of lignocelluloses. The method comprises the following steps: (1) a pretreated lignocellulose raw material and an enzyme solution are added in proportion into an enzymolysis reactor with a screw device to carry out continuous enzymolysis; (2) the materials enter a fermentation tank I after enzymolysis, and microzyme is inoculated to carry out simultaneous saccharification and fermentation at 30-38 DEG C; (3) the materials in the fermentation tank I enter a fermentation tank II, and simultaneous saccharification and fermentation are continuously carried out at 39-44 DEG C; (4) the fermented materials undergo reduced pressure distillation; and (5) the distilled materials undergo solid-liquid separation, and an enzyme-containing liquid phase is reused in the step (1). By the use of the screw device, continuous supply and continuous enzymolysis of the raw materials are realized, and enzymolysis efficiency is enhanced. As simultaneous saccharification and fermentation are carried out at different temperatures, enzymolysis efficiency is further improved and yield of ethanol is then raised. The finally-obtained enzyme solution can be directly reused in the enzymolysis process, thus reducing production cost.

Disclosed is a multiple adsorption tower alternate internal recycle method for producing anhydrous alcohol, which comprises the steps of heating, adsorbing, internal recycling, vacuum evacuating, flushing and resolving, the process has the advantages of extended adsorbent lifetime, reduced heat exchanger area, lowered cooling-water consumption, deep de-watering depth, and no pollution.

The invention relates to a method for producing ethanol by using sugarcane bagasse cellulose and sugarcane molasses. The method comprises the following steps: sugarcane bagasse pretreatment, enzyme hydrolysis and saccharification of sugarcane bagasse cellulose, sugarcane molasses pretreatment and mixed fermenting of sugarcane bagasse cellulose hydrolysate and the sugarcane molasses. Yeast strains are fixed to carry out ethanol fermentation by using double-enzyme saccharification sugarcane bagasse cellulose and an adsorption fixing material, the density of the yeast strains can be increased, furthermore, the yeast strains can be repeatedly used, sugar consumed by yeast propagation is reduced, fermentation strength and yield of products are improved, the yield of the ethanol reaches 93.5%, comprehensive utilization of sugarcane bagasse and molasses resources is realized, comprehensive economic benefit of sugarcane sugar producing enterprises can be improved remarkably, and a win-win situation of economic benefit and economic benefit is realized.

The invention discloses a Saccharomyces cerevisiae strain for expressing xylose isomerase and a construction method and solves the problem that after heterogenous expression of xylA gene in Saccharomyces cerevisiae, a recombinant bacterium constructed thereby has a low growth rate in xylose, causing the fermentation requirement to be not met. The invention includes a Saccharomyces cerevisiae strain SEB7, collected in China General Microbiological Culture Collection Center under CGMCC11327. The invention also provides a construction method of the Saccharomyces cerevisiae strain SEB7. The strain SEB7 obtained by the invention can generate ethanol 6.98 g / L within a fermenting time of 48 h by using xylose 16.95 g / L, with ethanol yield up to xylose 0.412 g / g; the strain has the advantages such as high xylose fermentation speed and high ethanol yield.

The invention relates to a Saccharomyces cerevisiae strain for producing ethanol by quick fermentation of xylose and a construction method, discloses a Saccharomyces cerevisiae strain capable of producing ethanol by fermenting glucose, xylose and cellobiose at the same time, and solves the problem that existing Saccharomyces cerevisiae cannot be used for producing ethanol by using xylose and cellobiose. The Saccharomyces cerevisiae strain SEB3 is collected in China General Microbiological Culture Collection Center under CGMCC11323. The invention also provides a construction method of the Saccharomyces cerevisiae strain SEB3. The Saccharomyces cerevisiae strain has the advantages of high xylose fermenting capacity, high xylose consumption speed, high ethanol yield and the like; meanwhile, the Saccharomyces cerevisiae strain SEB3 is better in acid resistance and temperature tolerance, and both pH not lower than 3 and temperature not higher than 35 DEG C have no significant impact on the fermenting speed and efficiency of xylose and cellobiose.

The invention discloses a method for improving the xylose utilization capacity of a recombinant saccharomyces cerevisiae strain by utilizing a transcription factor Tec1p mutant or for producing ethanol by utilizing xylose-containing raw materials. The method expresses the transcription factor Tec1p mutant in the recombinant saccharomyces cerevisiae with the xylose utilization capacity so as to improve the xylose utilization capacity of the strain; and the transcription factor Tec1p mutant is named as Tec1pT273M and is formed by mutating threonine at 273rd site of the wild-type transcription factor Tec1p with an amino acid sequence as shown in SEQ ID NO:1 into methionine, and the amino acid sequence of the Tec1pT273M is as shown in SEQ ID NO:2. Experiments prove that when the method is applied to glucose and xylose co-fermentation, the growth of the mutant strain is obviously superior to that of a starting strain BSGX001, and the utilization rate of xylose and the production rate of ethanol are obviously higher than that of the control strain.

The invention relates to a method for producing ethanol by utilizing bagasse hemicellulose. The method comprises the following steps of: pretreating bagasse, carrying out detoxification treatment on bagasse hemicellulose extracting solution, hydrolyzing the bagasse hemicellulose extracting solution and fermenting bagasse hemicellulose hydrolysate. Double enzyme saccharified bagasse hemicellulose and double yeast mixed strain are adopted for fermenting the bagasse hemicellulose diluted acid hydrolysate, the defect that the traditional saccharomyces cerevisiae can produce ethanol by utilizing glucose only while xylose fermentation can not be utilized is overcome, utilization rate of lignocellulosic materials is greatly improved, and the advantages of high ethanol yield, high conversion rate and the like are shown, so that the method provided by the invention is significant on production of bioethanol, comprehensive utilization of bagasse resource is realized, comprehensive economic benefit of a saccharose enterprise can be obviously improved, and double win in environmental benefit and economic benefit is realized.

The invention discloses a saccharomyces cerevisiae strain expressing xylose isomerase and a construction method. The saccharomyces cerevisiae (SEB8) strain expressing the xylose isomerase is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC11328. The invention further provides the construction method of the saccharomyces cerevisiae (SEB8). The obtained strain (SEB8) can generate ethanol with the concentration of 14.73 g / L from xylose with the concentration of 33.71 g / L in 48 hours of fermentation time, and the ethanol yield reaches 0.44 gram per gram of the xylose. The strain has the advantages of being high in xylose fermentation speed and ethanol yield and the like, can produce the fuel ethanol from saccharification liquid prepared by taking lignocellulose as a raw material, greatly reduces the production cost of the fuel ethanol and has a wide application prospect.

The invention relates to the technical field of spreading cultivation of microbial strains and fermentation of ethanol and discloses a method for spreading cultivation of saccharomycetes by virtue of liquefied mash, application of the method and a method for fermenting ethanol. The method for spreading cultivation of saccharomycetes by virtue of liquefied mash comprises the step of carrying out intermittent spreading cultivation and fed-batch spreading cultivation on activated saccharomycetes by utilizing liquefied mash filtrate under an aseptic condition. According to the method, the number of the saccharomycetes can be effectively controlled, the obtained saccharomycetes have relatively high activities, and the yield of ethanol and the consumption rate of xylose in a fermentation process can be obviously increased.

The invention relates to a method for improving ethanol production efficiency of lignocellulose enzymatic fermentation. The method comprises the following steps: firstly, uniformly mixing pretreated lignocellulose with water to prepare a mixed solution; then adding cellulase and cyclodextrin to the mixed solution for enzymolysis treatment; and inoculating an enzymatic hydrolysate with ethanol fermentation strains for ethanol fermentation. The method can reduce the influence of pretreatment inhibitors on enzymolysis and ethanol fermentation, and improve enzymolysis efficiency and enzymolysis sugar conversion rate.

The invention provides a method for preparing ethanol by using tobacco stem biorefinery. A pretreatment method specifically includes the steps: selecting fresh tobacco stems with tobacco leaves removed; airing, drying and chopping the fresh tobacco stems; adding NaOH solution with the concentration of 0.2%-5%; placing the solution into a water bath at the temperature of 80-90 DEG C for treatment for 30-120 minutes; washing and filtering the solution to a neutral state; collecting treated fibrous residues; drying the residues to obtain pretreated tobacco stem raw materials and then adding cellulase accounting for 10-30% of the weight of the raw materials and adding sodium acetate buffer solution with the pH (potential of hydrogen) of 4.8 and the concentration of 50 mmol / L; inoculating activated yeast solution in a constant temperature water bath at the temperature of 40-50 DEG C for 48-72 hours, and collecting filtrate to obtain ethanol solution. According to the method, the yield of ethanol obtained by enzymatic saccharification and fermentation can reach 106.64 mg / g which is increased by 138% as compared with that of the untreated tobacco stems. The utilization rate of the tobaccostems is greatly increased.

The invention discloses a Saccharomyces cerevisiae strain tolerant to vanillic acid, p-hydroxybenzoic acid and syringic acid and application of the Saccharomyces cerevisiae strain. The Saccharomyces cerevisiae strain is a strain PAT01 which is obtained after Saccharomyces cerevisiae is subjected to adaptive evolution by a phenolic acid inhibitor culture medium and can tolerate one or more of the vanillic acid, the p-hydroxybenzoic acid and the syringic acid with classification and nomenclature of Saccharomyces cerevisiae, the Saccharomyces cerevisiae is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 18021. The Saccharomyces cerevisiae strain can be applied to biomass conversion, and in saccharification and ethanol fermentation processes, the glucose consumption rate, the ethanol production rate and the ethanol yield of the Saccharomyces cerevisiae strain are all higher than those of an original strain.

The invention discloses a novel health wine and a preparation method thereof. The health wine is prepared by adopting fried rice, milky tea and shredded meat as base materials. The preparation method comprises the following steps of: piling 60-80 parts of fried rice, adding 20-30 parts of fresh milky tea at 90 DEG C and adding hot water for stacking and fermenting; adding shredded meat juice with mixture total weight of 1-2 percent for stewing; charging in a pool for fermenting, discharging out of the pool to fill the mixture into a steamer, brewing, distilling, optimizing and combining a wine body and fine filtering to obtain the health wine. The invention has natural and pure taste and low ester and aldehyde content, and is suitable for different crowds to drink healthily.

The invention provides a method for promoting production of ethanol from poplar through hydrothermal and NaOH aqueous solution pretreatment. The method comprises the following steps: crushing poplar, reacting with water at 180-200 DEG C for 5-30 minutes, mixing filter residues obtained by separation with a NaOH aqueous solution, reacting at 80-120 DEG C for 30-120 minutes, separating to obtain pretreated residues, adding cellulase, a saccharomycetes activating solution and a nutrient solution, and carrying out diastatic fermentation. According to the method, a hydrothermal and NaOH aqueous solution two-section pretreatment combined mode is adopted, the influence of lignin on the enzymolysis and fermentation process is reduced, on the premise that the alkali concentration is reduced, production of ethyl alcohol from poplar can be effectively promoted, and the ethyl alcohol yield reaches up to 50.91%.

The invention belongs to the technical field of biology, and discloses a method for in-situ immobilization of carbon dioxide in an ethanol fermentation process, which comprises the following steps: (1) constructing recombinant Saccharomyces cerevisiae-FDH for expressing formate dehydrogenase; (2) determining the carbon dioxide fixed amount and the ethanol yield of the recombinant strain S.cerevisiae-FDH under different glucose concentrations; (3) the carbon dioxide fixed amount and the ethanol yield of the recombinant strain S.cerevisiae-FDH under different inoculum sizes are determined; and (4) adding a carbon dioxide cosolvent such as 2-methylimidazole zinc salt MAF-4 to improve the fixing efficiency of carbon dioxide. The method is simple and easy to implement, does not affect the main fermentation process and microbial growth, utilizes carbon dioxide released in the ethanol fermentation process in situ, and achieves the purposes of carbon dioxide emission reduction and total carbon recovery rate improvement.

The invention relates to a method for producing ethanol by continuous enzymolysis and fermentation of lignocelluloses. The method comprises the following steps: (1) a pretreated lignocellulose raw material and an enzyme solution are added in proportion into an enzymolysis reactor with a screw device to carry out continuous enzymolysis; (2) the materials enter a fermentation tank I after enzymolysis, and microzyme is inoculated to carry out simultaneous saccharification and fermentation at 30-38 DEG C; (3) the materials in the fermentation tank I enter a fermentation tank II, and simultaneous saccharification and fermentation are continuously carried out at 39-44 DEG C; (4) the fermented materials undergo reduced pressure distillation; and (5) the distilled materials undergo solid-liquid separation, and an enzyme-containing liquid phase is reused in the step (1). By the use of the screw device, continuous supply and continuous enzymolysis of the raw materials are realized, and enzymolysis efficiency is enhanced. As simultaneous saccharification and fermentation are carried out at different temperatures, enzymolysis efficiency is further improved and yield of ethanol is then raised. The finally-obtained enzyme solution can be directly reused in the enzymolysis process, thus reducing production cost.

The invention relates to the technical field of spreading cultivation of microbial strains and fermentation of ethanol and discloses a method for spreading cultivation of saccharomycetes by virtue of liquefied mash, application of the method and a method for fermenting ethanol. The method for spreading cultivation of saccharomycetes by virtue of liquefied mash comprises the step of carrying out intermittent spreading cultivation and fed-batch spreading cultivation on activated saccharomycetes by utilizing liquefied mash filtrate under an aseptic condition. According to the method, the number of the saccharomycetes can be effectively controlled, the obtained saccharomycetes have relatively high activities, and the yield of ethanol and the consumption rate of xylose in a fermentation process can be obviously increased.

The invention relates to a method for producing fuel ethanol by sugar grass stalk juice and a device; the stalk pretreatment, juice modulation, immobilization yeast particles and fluidization quick fermentation new processes are adopted to prepare the fuel ethanol; the fermentation process is continuous, the equipment is advanced, the fermentation speed is rapid, the reaction is full, the output is high, and the ethanol yield is high, no three-waste exhaust exists, secondary utilization is carried out totally, a great amount of investment, labor cost and raw material cost are reduced, the economic benefit is improved obviously, and the production cost is greatly reduced.

The invention relates to a method for producing fuel ethanol by sugar grass stalk juice and a device; the stalk pretreatment, juice modulation, immobilization yeast particles and fluidization quick fermentation new processes are adopted to prepare the fuel ethanol; the fermentation process is continuous, the equipment is advanced, the fermentation speed is rapid, the reaction is full, the output is high, and the ethanol yield is high, no three-waste exhaust exists, secondary utilization is carried out totally, a great amount of investment, labor cost and raw material cost are reduced, the economic benefit is improved obviously, and the production cost is greatly reduced.

The invention discloses a saccharomyces cerevisiae strain expressing xylose isomerase and a construction method. The saccharomyces cerevisiae (SEB8) strain expressing the xylose isomerase is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC11328. The invention further provides the construction method of the saccharomyces cerevisiae (SEB8). The obtained strain (SEB8) can generate ethanol with the concentration of 14.73 g / L from xylose with the concentration of 33.71 g / L in 48 hours of fermentation time, and the ethanol yield reaches 0.44 gram per gram of the xylose. The strain has the advantages of being high in xylose fermentation speed and ethanol yield and the like, can produce the fuel ethanol from saccharification liquid prepared by taking lignocellulose as a raw material, greatly reduces the production cost of the fuel ethanol and has a wide application prospect.

The invention belongs to the technical field of cellulosic ethanol production, and particularly relates to a method for producing ethanol by co-fermentation of glucose and xylose. The method comprises the following steps of: (1) strain selection; (2) slant culture; (3) seed culture; (4) seed expanding culture; (5) fermentation culture; and (6) distillation of the fermentation liquid obtained in the step (5) to obtain ethanol. By using the material enzymolysis liquid obtained by the widely used steam explosion pretreatment technique as the culture medium, on the basis of directional stress resistance and fermentation property domestication of the pentose yeast strain, the strain is utilized to perform ethanol fermentation; and in the high-concentration acetic acid and furfural environment, the glucose and xylose are synchronously fermented to produce ethanol, so that the glucose utilization ratio is up to 99.5% or above, the xylose utilization ratio is up to 70% or above, the ethanol yield is up to 85% of the theoretical value, and the raw material utilization ratio and ethanol yield are effectively enhanced.

The invention discloses an alcohol production method, which comprises the following steps of: (1) respectively carrying out liquefaction and saccharification on farinaceous size, and fermenting a saccharification liquid obtained by saccharification to obtain CO2-containing gas component A and a fermented liquor; (2) letting the CO2-containing gas component A contact with a washing liquid, washing, dissolving ethanol from the CO2-containing gas component A in the washing liquid to obtain a complete liquid; and carrying out solid-liquid separation on the fermented liquor to obtain a fermented clear liquid; (3) carrying out distillation on the complete liquid and the fermented clear liquid to obtain a gaseous distillate B, condensing the gaseous distillate B until ethanol is a liquid state, so as to obtain a liquid ethanol fraction C and a gas component D; and (4) letting the gas component D contact with water, and washing to obtain a washed liquid E. The invention is characterized in that the washing liquid comprises the washed liquid E and the concentration of ethanol in the washing liquid is not higher than 2.5% (volume). The alcohol production method provided by the invention is used to raise ethanol yield and reduce cost.

The invention discloses a method for improving DDGS (distillers dried grains with soluble) quality in a corn ethanol process through secondary fermentation of distillers' grains. According to a traditional corn ethanol production process, corn flour is used as a raw material, and fuel ethanol and vinasse are obtained through liquefaction, simultaneous saccharification and fermentation and distillation processes. On the basis of the corn ethanol production process, the obtained distillers' grains are subjected to secondary synchronous saccharification and fermentation, the content of cellulose, residual starch and residual glucose in the distillers' grains is reduced, the ethanol yield is increased, meanwhile, the strength of a Maillard reaction in the DDGS preparation process is weakened, and then the DDGS with the improved quality is obtained. According to the method, the color and luster of the DDGS can be improved, the quality of the DDGS is improved, and the market competitiveness of the DDGS and the technical economy of an original corn ethanol process are enhanced.

The present invention discloses a Saccharomyces cerevisiae strain and construction method that can rapidly ferment xylose to produce ethanol, discloses a Saccharomyces cerevisiae strain that can simultaneously ferment glucose, xylose and cellobiose to produce ethanol, and solves the problem of Saccharomyces cerevisiae The problem of inability to produce ethanol from xylose and cellobiose. The invention includes that the strain of Saccharomyces cerevisiae SEB3 is preserved in China General Microorganism Culture Collection and Management Center, and the preservation number is CGMCC11323. The invention also provides the construction method of the Saccharomyces cerevisiae SEB3. The present invention has the advantages of excellent xylose fermentation ability, fast xylose consumption rate and high ethanol yield; at the same time, the bacterial strain SEB3 of the present invention has good acid resistance and temperature resistance, and the pH value is not lower than 3 and the temperature is not high. At 35°C, pH and temperature had no significant effect on the fermentation rate and efficiency of xylose and cellobiose.

The invention discloses an industrial alcohol reflux re-fermentation process. The process comprises a fermentation tank, a return pipeline connected with the top of the fermentation tank, a first condenser connected with the return pipeline, a recovery pipeline used for collecting pipeline flushing water, a pipeline alcohol storage tank connected with the recovery pipeline, a blow-off pipe arranged below the pipeline alcohol storage tank, a delivery pump connected with the pipeline alcohol storage tank, a preheater connected with the conveying pump, a coarse distillation tower connected with the preheater, a rectifying tower connected with the coarse distillation tower, a second condenser connected with the rectifying tower, an alcohol circulating pump connected with the second condenser, a buffer tank connected with the alcohol circulating pump and a finished product storage tank connected with the buffer tank. The backflow impurities such as acetaldehyde and acetic acid can participate in the reaction again to generate ethanol, so that the yield of ethanol is increased, and the generation of impurities is reduced.

The invention discloses a method for improving the xylose utilization ability of a recombinant Saccharomyces cerevisiae strain by using a transcription factor Tec1p mutant or using xylose-containing raw materials to produce ethanol. To improve the ability of strain xylose utilization; the transcription factor Tec1p mutant is named as Tec1p T273M , is formed by mutating the threonine at position 273 of the wild-type transcription factor Tec1p whose amino acid sequence is SEQ ID NO:1 to methionine, and its amino acid sequence is shown in SEQ ID NO:2. Experiments show that the growth of the mutant strain is significantly better than that of the starting strain BSGX001 in the co-fermentation of glucose and xylose by the method of the present invention, and the utilization of xylose and the production rate of ethanol are significantly higher than those of the control strain.

The invention low temperature-resistant Saccharomyces cerevisiae JM33 and application of the low temperature-resistant Saccharomyces cerevisiae JM33 in munake grape wine. According to the low temperature-resistant Saccharomyces cerevisiae JM33 disclosed by the invention, by carrying out strain separating, screening, breeding and acclimatizing on munake grapes and carrying out morphological characteristic and 26S rDNA (Ribosomal Deoxyribose Nucleic Acid) sequence determination and phylogenetic analysis on an obtained strain, a fermentation action of strain Saccharomyces cerevisiae JM33 CGMCC NO.10912 on the munake grapes is determined; the munake grape wine is prepared by fermenting the munake grapes by utilizing the Saccharomyces cerevisiae JM33 CGMCC NO.10912, the munake grape wine is straw-like yellow and is clear and transparent, the fruity flavor is strong, the taste is mellow and soft, and a special flavor of the munake grape wine is obtained.