Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for increasing the yield of ethanol produced by enzymatic fermentation of lignocellulose

A lignocellulosic enzyme and lignocellulose technology, applied in the field of lignocellulose enzymatic fermentation and fermentation to prepare fuel ethanol, can solve the problems of high production cost, low ethanol yield, low fermentation efficiency, etc., and achieve low price, abundant sources, Effect of increasing ethanol yield

Active Publication Date: 2021-08-27
SHANDONG UNIV OF SCI & TECH
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the problems of low efficiency of enzymatic hydrolysis and fermentation of lignocellulose, low yield of ethanol, and high production cost, the object of the present invention is to provide a method to improve the yield of ethanol produced by enzymatic hydrolysis and fermentation of lignocellulose by using lignin amphiphilic polymer as an auxiliary agent. rate method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for increasing the yield of ethanol produced by enzymatic fermentation of lignocellulose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 2.5 g of wine yeast powder was placed in a sterile sugar water of 50 ml of concentration of 20 g / L, and 40 min was activated at 35 ° C as yeast seed liquid.

[0027] Putting 100 moisture dilute sulfuric acid was added to 5,000 mass pH = 6.5 of acetic acid-acetate buffer, add 50 mass of lignozo sulfonate (pulp paper by-product), 5FPU / g The cellulase (gauge in the quality of the river), then the 8-position yeast seed fluid was added.

[0028] The above mixture was synchronized at 45 ° C for synchronous saccharification, and the fermentation time was 48 h, and two fermented ethanol content were measured by a liquid chromatography, and the ethanol yield was calculated, and the statistical results were shown in Table 1.

Embodiment 2

[0030] 2.5 g of wine yeast powder was placed in a sterile sugar water of 50 ml of concentration of 20 g / L, and 40 min was activated at 35 ° C as yeast seed liquid.

[0031] Add 100-mass sulfite preprocessing pine wood to 5,000 mass pH = 4.5 acetic acid-acetate buffer, 40-mass sulfite pretreatment liquid, 20FPU / g of cellulase (cellulase) With the quality of the rocky loose wood, then 8-quality yeast seed fluid was added.

[0032] The above mixture was synchronized at 30 ° C for synchronous saccharification, and the fermentation time was 120 h, and two fermented ethanol content was measured by a liquid chromatography, and the ethanol yield was calculated, and the statistical results were shown in Table 1.

Embodiment 3

[0034] 2.5 g of a Saccharomycesmine was placed in a sterile sugar water having a concentration of 20 g / L, and 30 min was activated at 35 ° C as a yeast seed.

[0035] The corn stalks treated with 100 mass of the organic solvent were added to 2500 mass pH = 5.5 of acetic acid-acetate buffer, add 30 mass of quaternized lignin sulfonate, 10FPU / g of cellulase ( The mass of the tannous corn stalk was then added 5 quality yeast seed fluid.

[0036] The above mixture was synchronized at 35 ° C for synchronous saccharification, and the fermentation time was 120 h, and two fermented ethanol content were measured by a liquid chromatography, and the ethanol yield was calculated, and the statistical results were shown in Table 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of preparing fuel ethanol by enzymatic hydrolysis and fermentation of lignocellulose, and discloses a method for improving the yield of ethanol produced by enzymatic hydrolysis and fermentation of lignocellulose by a lignin amphiphilic polymer. The specific steps of the method are: dispersing 100 mass parts of pretreated lignocellulose in a buffer solution of 330 to 5000 mass parts, adding 0.5 to 50 mass parts of lignin amphiphilic polymer, and then adding 5 to 30 FPU / g cellulase (based on the mass of dry lignocellulose), and then add Saccharomyces cerevisiae, and react at 30-45°C for 48-120h to obtain a fermentation broth. The invention can not only effectively improve the ethanol yield of lignocellulose enzymolysis fermentation, but also does not need additional equipment, and has simple process and convenient operation.

Description

Technical field [0001] The present invention relates to the field of lignocellulose enzymatic fermentation, and in particular, in particular, to a method of promoting lignin cellulose enzymatic fermentation ethanol using lignin amphiphilic polymers. Background technique [0002] With the increasing depletion of traditional fossil energy, humans are facing the crisis of energy shortages, resource lack, and fossil fuels have caused severe pollution in the ecological environment in the use process. Therefore, seeking green, naturally renewable cleaning energy is a general trend in instead of traditional fossil fuel. The fuel ethanol which is concerned in the field of biomass energy can reduce the amount of gasoline, so that the combustion of gasoline is more fully sufficient to reduce the emissions of harmful gases. Wooden cellulose production fuel ethanol usually includes a process such as pretreatment, enzymatic hydrolysis, fermentation, distillation, but at present, the process i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/10C12R1/865
CPCC12P7/10C12P2201/00Y02E50/10
Inventor 周海峰史欣欣田原宇乔英云赵国明刘庆梁缘
Owner SHANDONG UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com