The invention discloses a broad-spectrum bactericidal difenoconazole thermal fog formulation and a preparation method thereof. The difenoconazole thermal fog formulation contains difenoconazole, a surface active agent, a solvent, a synergistic agent and a stabilizing agent, and the weight percentages are as follows: 1-40% of difenoconazole, 5-30% of surface active agent, 0.1-3% of synergistic agent, 0.01-2% of stabilizing agent, and remaining solvent. The invention has the characteristics of good and fast effects, labor saving, drug saving, no limitations of landforms, tree heights and water sources, convenient application, and the like, which is applied to controlling various plant diseases of crops in forested areas, gardens and greenhouse rooms, such as powdery mildew, scab, anthrax, rust disease, leaf spot, etc.