The invention relates to a printing and reinforcement method of a 3D printing concrete member, which comprises the following steps: (1) determining a mix proportion: preparing concrete suitable for a 3D printing structure, fully mixing, testing related printing performance, and meeting various performance requirements, otherwise, adjusting or repreparing until the requirements are met; (2) according to design requirements, relevant parameters of components, relevant parameters of equipment and relevant parameters of reinforcing bars are determined; and (3) after single-layer concrete is printed, rib grids are laid, concrete layer printing and rib grid laying are repeated till a complete spliced rib cage is formed, and finally concrete is covered to form a complete component. According to the method, the influence of 3D printing anisotropy on the 3D printing concrete structure is relieved through the staggered joint printing method, meanwhile, the reinforcing bars integrating the tensile longitudinal bars, the erection bars and the stirrups are configured, the assembled bar cage is formed, and the stress performance of the 3D printing concrete structure is improved.