The present invention is a blood collection needle holder, which comprises a sleeve, a blood collection needle connection seat, the blood collection needle connection seat is movably installed on the sleeve end surface recessed between two fixed ear seats at the front end of the sleeve, and the sleeve end surface is also provided with There is a central through hole and several cylinders protruding forward. The connecting seat of the blood collection needle is a rectangular frame with an elastic card in the middle. The elastic card for fixing the blood collection needle is arranged in the rectangular frame. The symmetrical elastic cards are on the protruding cylinder. Under the positioning, the blood collection needle fixing ring is formed, and the blood collection needle is connected and fixed at the front end of the sleeve. After the blood collection is completed, push the blood collection needle connection seat, the semicircular snap ring of the elastic card breaks away from the limit, and automatically opens, so that the blood collection needle can be conveniently and quickly separated from the blood collection needle connection seat with one hand. The invention has reasonable structure, convenient loading and unloading of the blood collection needle, simple operation, avoids cross-infection caused by accidental stabbing of the needle, and has good safety.