The invention discloses an antibacterial anti-mildew self-cleaning nanometer antifouling coating material, a preparation method and applications thereof, wherein the coating material comprises a component A and a component B, the component A contains an organofluorine resin, an organic-inorganic composite nanometer antibacterial agent, a nanometer ceramic particle, nanometer titanium dioxide, a pigment filler, an anti-precipitating agent, a dispersing agent, a leveling agent and a diluent, and the component B comprises a curing agent. According to the present invention, the coating material has advantages of excellent antibacterial and anti-mildew performance and excellent self-cleaning property; by combining the composite antibacterial agent comprising nanometer silver and a zwitterionicpolymer with nanometer titanium dioxide, the synergistic bactericidal effect can be achieved; by combining a low surface energy organofluorine resin and a nanometer material, the coating has advantages of super-strong hydrophobicity and super-strong oil stain resistance, can greatly reduce the contact area between water stains, dust and microbial bodies and the surface of the matrix, can easily achieve the rapid shedding of surface pollutants, and has excellent self-cleaning property.