The invention discloses a
digital holography-based three-dimensional micro-observation apparatus for
cell dynamic of a living
organism. According to the apparatus provided in the invention, a microscopic technology of
digital holography is employed, so that dynamic three-dimensional
microscopic observation without contact, damage and pretreatment on a
cell of a living
organism can be realized; a
fiber optical path is utilized to realize object light illumination and an
optical path portion of the object light employs a vertical type structure, so that it is convenient to carry out observationon the
cell of the living
organism; reference light utilizes a
light beam turnover device to control an included angle between the reference light and the object light so as to realize off-axis digitholography; and an infinite
distance correction microscopic object lens is employed to carry out pre-amplification and imaging on the cell; simultaneously, a
relay lens is utilized to adjust an imaging position and a
wavefront curvature of the object light, so that observation resolution is improved. According to the apparatus,
layout structures of a plurality of optical elements are compact, flexible and stable thorough a platform; moreover, the apparatus can be applied to dynamic observation on the cell of the living organism for a long time.