33 results about "Salicylic acid synthesis" patented technology

The present invention discloses an alkyl salicylic acid synthesis method, which comprises: mixing olefin with a carbon number of 4-50 and salicylic acid, and carrying out an alkylation reaction under a catalysis effect of aryl sulfonic acid at a temperature of 50-160 DEG C. According to the present invention, aryl sulfonic acid is adopted as a catalyst, a conversion rate can achieve 60-95%, the catalyst does not cause corrosion on the equipment, price is relatively low, the catalyst can be recycled, no three-waste emission problem exists, and environmental protection is easily achieved.

Disclosed is a novel supercritical phase salicylic acid synthesis process, which comprises making sodium phenolate through neutralizing phenol with NaOH aqueous solution, drying and grinding to obtain solid sodium phenolate, subjecting the sodium phenolate and carbon dioxide to supercritical homogeneous phase carboxylation reaction (Kolbe-Schmitt reaction) in heavy pressure reaction kettle, obtaining sodium salicylate, acidifying to prepare the salicylic acid.

The invention discloses the method of improving the content of the salicin in the plant and the appropriative carrier. The carrier is the plant expressing carrier containing the separating offset acid composing enzyme gene and the separating offset acid-the pyruvic splitting dividing enzyme gene. The method uses the salicin composing approach and guides the different ICS gene and the IPL gene into the plant. The invention guides the said carrier into the plant group or the cell to gain the turning gene plant of the content of the salicin improved. The transferring gene test indicates that the dissociating state SA content in the transferring gene rice is mostly nine times than the wildness type. The method of the invention and the appropriative carrier can improve the content of the salicin in the plant greatly and provides the new method of amending the plant breed and improving the crop.

The invention discloses a plant resistance-related protein ATWRKY46 as well as an encoding gene and application thereof. The protein provided by the invention is named ATWRKY46 protein, which is from Arabidopsis thaliana and is any one of the following: (a) the protein formed by amino acid sequences as shown in a sequence 1; and (b) the protein which is derived from the sequence 1 in a way that the amino acid sequences as shown in the sequence 1 are subjected to replacement and / or deletion and / or addition of one or a plurality of amino acid residues, and has any function of the following: inducing a starting function of an ICS1 gene promoter, and inducing a starting function of a PR1 (pathogenesis-related protein) gene promoter, and the protein which is related to synthesis of salicylic acid in a plant and plant resistance and is derived from the sequence 1. The ATWRKY46 protein provided by the invention can increase disease resistance of the plant through regulating and controlling the synthesis of the salicylic acid in the plant. The invention is of great value to cultivate disease-resistant plants.

The invention discloses the method of improving the content of the salicin in the plant and the appropriative carrier. The carrier is the plant expressing carrier containing the separating offset acid composing enzyme gene and the separating offset acid-the pyruvic splitting dividing enzyme gene. The method uses the salicin composing approach and guides the different ICS gene and the IPL gene into the plant. The invention guides the said carrier into the plant group or the cell to gain the turning gene plant of the content of the salicin improved. The transferring gene test indicates that the dissociating state SA content in the transferring gene rice is mostly nine times than the wildness type. The method of the invention and the appropriative carrier can improve the content of the salicin in the plant greatly and provides the new method of amending the plant breed and improving the crop.

The present invention discloses an alkyl salicylic acid synthesis method, which comprises: mixing olefin with a carbon number of 4-50 and salicylic acid, and carrying out an alkylation reaction under a catalysis effect of aryl sulfonic acid at a temperature of 50-160 DEG C. According to the present invention, aryl sulfonic acid is adopted as a catalyst, a conversion rate can achieve 60-95%, the catalyst does not cause corrosion on the equipment, price is relatively low, the catalyst can be recycled, no three-waste emission problem exists, and environmental protection is easily achieved.

The invention discloses a plant resistance related protein ANAC001, its encoding gene and an application thereof. The protein provided by the invention is named as ANAC001 protein, comes from Arabidopsis thaliana, and is any one of the following proteins: (a) a protein formed by an amino acid sequence as shown in the sequence 1; (b) a protein which is derived from the sequence 1 by replacing and / or deleting and / or adding one or more amino acid residues in the amino acid sequence of the sequence 1, and has any one of the following functions: a function of inducing ICS1 gene promoter, a function of inducing PR1 gene promoter, a function of being related with salicylic acid synthesis in plant and a function of being related with plant resistance. The ANAC001 protein provided by the inventioncan regulate and control the synthesis of salicylic acid in a plant so as to enhance plant disease resistance. The invention has a great value for the cultivation of disease resistant plants.

The invention belongs to the technical field of cotton plant protection, and particularly relates to a cotton immune system activator and an application thereof. According to the invention, a mutual relationship between an exogenous activator and a cotton immune system is researched by means of a molecular biology, and the result shows that indole can be used as a cotton immune system activator. After an indole solution with a concentration of 200 [mu]M or above is externally applied, the resistance of cottons to verticillium wilt can be remarkably enhanced. The whole genome transcriptome is analyzed, and the result shows that the expression of genes related to disease resistance, salicylic acid synthesis and signal path in a cotton immune system is remarkably activated after treatment byusing indole. The cotton immune system activator can be developed into a novel reagent for preventing and controlling cotton verticillium wilt, cotton seedlings are treated by spraying or drip irrigation, and the resistance of cottons to the verticillium wilt can be improved.

The invention relates to a one-step method for synthesizing salicylic acid with phenol and supercritical carbon dioxide, and relates to the technical field of salicylic acid synthesis. According to the method, supported potassium carbonate is adopted as a catalyst, and salicylic acid is synthesized with phenol and supercritical carbon dioxide. According to the invention, supported potassium carbonate is adopted as the catalyst, such that the product can be easily separated from the catalyst. A traditional process flow is simplified, and pickling and washing processes in the traditional processes are eliminated, such that the method is a green and clean production process. With the method, phenol conversion rate and salicylic acid selectivity are improved, and continuous production is realized. With the method, the phenol conversion rate can reach 100%, and the salicylic acid selectivity is up to 99.3%.

The invention discloses a plant resistance related protein ATSAR25, and encoding genes and application thereof. The protein provided in the invention is named ATSAR 25, originates from Arabidopsis thaliana, and is one selected from the following proteins: (a) a protein composed of an amino acid sequence represented by sequence 1; (b) a protein derived from sequence 1, wherein, the protein is obtained by substituting and / or deleting and / or adding one or more deoxynucleotide residue of the amino acid sequence of sequence 1 and has either the function of inducing promotion of ICS 1 gene promoters or the function of inducing promotion of PR 1 gene promoters; (c) a protein which is derived from sequence 1 and related to synthesis of salicylic acid in plants and to plant resistance. The ATSAR 25 protein provided in the invention can enhance disease resistance of plants and has a critical value to breeding of disease resistant plants.

An efficient synthesis method of salicylic acid belongs to the technical field of salicylic acid synthesis. The dehydration method adopted by the invention comprises the following steps: vacuumizing a sodium phenate solution for primary dehydration, adding phenol into the sodium phenate solution when the water content of the sodium phenate solution reaches about 45% such that a part of the phenol and water form a binary azeotrope, and separating the binary azeotrope under negative pressure condiction to achieve the purpose of taking out water by the phenol. The residual phenol and the sodium phenate form the bulky compound under the vacuum condition, and the bulky compound has the large specific surface area, such that the reaction rate is accelerated, the reaction time is substantially shortened, and the production efficiency is improved when the CO2 is introduced to perform the carboxylation reaction; meanwhile, the binary azeotrope and phenol separated in the process can be recycled, so that resources are saved, and the cost is reduced.

The invention provides a preparation method of an aralkyl salicylic acid derivative. The method is characterized in that styrene or a derivative thereof reacts with salicylic acid or a derivative thereof in the presence of an ionic liquid catalyst. The method overcomes the disadvantages of present aralkyl salicylic acid synthesis technologies, and is an efficient and green aralkyl salicylic acid synthesis technology. The catalyst adopted in the invention settles to the bottom of a reactor after the reaction ends, is convenient to separate, and also has the advantages of multi-time repeated use, no emission of waste acids, and environment protection benefiting in case of guaranteeing good yield.

The invention discloses distillation equipment for synthesizing acid salicylic acid and a distillation method of the distillation equipment. The distillation equipment comprises a base, a distillation shell, a mounting shell, a distillation mechanism and a cooling mechanism, wherein the top side surface of the base is fixedly connected with a distillation shell, the top side of the distillation shell is provided with a mounting shell, and the side surface of the mounting shell is fixedly communicated with a feeding pipe; the distillation mechanism comprises a servo motor, a stirring shaft, a stirring rod and a heating plate, the servo motor is fixedly connected to the surface of the top side of the mounting shell, the output end of the servo motor is connected with the stirring shaft, the stirring rod is fixedly connected to the side surface of the stirring shaft, the heating plate is placed in the distillation shell, and a baffle plate is fixedly connected to the inner wall of the distillation shell. And through holes are formed in the side surfaces of the baffle plates. According to the distillation equipment for synthesizing the acid-based salicylic acid and the distillation method of the distillation equipment, the design is novel, the structure is simple, heating distillation can be conveniently carried out on acid-based salicylic acid liquid, liquid in the distillation shell can be conveniently stirred, and the distillation effect is improved.

The invention discloses a plant resistance related protein MAG, and a coding gene and application thereof. The protein disclosed by the invention is named MAG protein, comes from Arabidopsis thaliana, and is (a) a protein composed of an amino acid sequence shown in sequence 1; or (b) a protein derived from sequence 1, which is formed by the substitution and / or deletion and / or addition of one or more amino acid residues in the amino acid sequence shown in sequence 1 and has any function of inducing the activation of an ICS1 gene promoter, inducing the activation of a PR1 gene promoter, being related to the synthesis of salicylic acid in plants or being related to the plant resistance. The MAG protein disclosed by the invention can enhance the disease resistance of plants by regulating and controlling the synthesis of salicylic acid in the plants. The invention has an important value for the cultivation of disease-resistant plants.

The invention discloses a Chinese medicine composition anti-wrinkle multi-function liquid, its preparation and application. The anti-wrinkle multi-function liquid comprises the following ingredients: 11-13 weight portions of the root of kudzu vine, 12-14 weight portions of rehmannia glutinosa, 7-9 weight portions of gingko, 11-13 weight portions of dark plum, 12-14 weight portions of leonurus, 4-6 weight portions of radix salvia miltiorrhizae, 6-8 weight portions of liquorice, 7-9 weight portions of Ligusticum wallichii, 6-8 weight portions of Angelica sinensis, 5-7 weight portions of Semen Cuscutae, 3-5 weight portions of glossy ganoderma, 4-6 weight portions of rhizoma cyperi, and 8-10 weight portions of ginseng. The multi-function liquid is light yellow and tasteless. The trade name ofthe multi-function liquid is aromlotus Chinese medicine composition anti-wrinkle multi-function liquid. The direct use of the multi-function liquid on the skin can effectively remove wrinkles, and can be absorbed by the skin effectively, so that the effects of skin care and skin beauty are achieved. According to the invention, no chemical additives are added in the multi-function liquid, and the multi-function liquid uses all natural extracts, so that the effects are realized depending on the Chinese medicine extracts completely; and the natural extracts are obtained by mixed extraction according to Chinese medicine formula, so that the characteristics of the compatibility of Chinese medicines are really shown.

The invention relates to a reagent (kit) for diagnosing / determining amino acid by using an enzyme colorimetric method and an enzyme link method, and also discloses a method for determining the concentration of the amino acid, a composition and components of the reagent, belonging to the technical field of medicine / food / environmental test determination. The reagent (kit) comprises the main components: a buffer solution, a coenzyme, sodium bicarbonate (carbon dioxide), an acetylcoenzyme A, 6-gaultherolin, an amino acid oxidase, a pyruvic oxidase, a 6-methyl salicylate synthetase and a stabilizing agent. A sample and the reagent are mixed according to a certain volume ratio to generate a series of enzymatic reactions, and reactants are placed under an ultraviolet / visible light analyzer for detecting the degree of absorbance rise at the dominant wavelength of 340 nm, thereby measuring and calculating the concentration of the amino acid.

The invention provides a preparation method of an aralkyl salicylic acid derivative. The method is characterized in that styrene or a derivative thereof reacts with salicylic acid or a derivative thereof in the presence of an ionic liquid catalyst. The method overcomes the disadvantages of present aralkyl salicylic acid synthesis technologies, and is an efficient and green aralkyl salicylic acid synthesis technology. The catalyst adopted in the invention settles to the bottom of a reactor after the reaction ends, is convenient to separate, and also has the advantages of multi-time repeated use, no emission of waste acids, and environment protection benefiting in case of guaranteeing good yield.

The invention discloses a plant resistance-related protein ATWRKY46 as well as an encoding gene and application thereof. The protein provided by the invention is named ATWRKY46 protein, which is from Arabidopsis thaliana and is any one of the following: (a) the protein formed by amino acid sequences as shown in a sequence 1; and (b) the protein which is derived from the sequence 1 in a way that the amino acid sequences as shown in the sequence 1 are subjected to replacement and / or deletion and / or addition of one or a plurality of amino acid residues, and has any function of the following: inducing a starting function of an ICS1 gene promoter, and inducing a starting function of a PR1 (pathogenesis-related protein) gene promoter, and the protein which is related to synthesis of salicylic acid in a plant and plant resistance and is derived from the sequence 1. The ATWRKY46 protein provided by the invention can increase disease resistance of the plant through regulating and controlling the synthesis of the salicylic acid in the plant. The invention is of great value to cultivate disease-resistant plants.

The invention discloses a plant resistance related protein ZIP11, its encoding gene and an application thereof. The protein provided by the invention is named as ZIP11 protein, comes from Arabidopsis thaliana, and is any one of the following proteins: (a) a protein formed by an amino acid sequence as shown in the sequence 1; (b) a protein which is derived from the sequence 1 by replacing and / or deleting and / or adding one or more amino acid residues in the amino acid sequence of the sequence 1, and has any one of the following functions: a function of inducing ICS1 gene promoter, a function ofinducing PR1 gene promoter, a function of being related with salicylic acid synthesis in plant and a function of being related with plant resistance. The ZIP11 protein provided by the invention can regulate and control the synthesis of salicylic acid in a plant so as to enhance plant disease resistance. The invention has a great value for the cultivation of disease resistant plants.

The invention relates to an ammonia (ammonia ion) content determination method, and composition and ingredients of reagents adopted by the ammonia (ammonia ion) content determination method. The ammonia (ammonia ion) content determination method adopts an enzymatic colorimetry and an enzyme-linked immunosorbent assay technology. A technical principle of the ammonia (ammonia ion) content determination method comprises that determination is realized by a series of catalytic reactions of ammonia kinase, propionyl CoA carboxylase and 6-methyl salicylate synthase. The invention also relates to an ammonia (ammonia ion) diagnosis / determination kit. The ammonia (ammonia ion) content determination method provided by the invention has high sensitivity and a small error. The ammonia (ammonia ion) content determination method and the ammonia (ammonia ion) diagnosis / determination kit can be widely utilized for clinical medicine and food examination.

The invention relates to an ammonia (ammonia ion) content determination method, and composition and ingredients of reagents adopted by the ammonia (ammonia ion) content determination method. The ammonia (ammonia ion) content determination method adopts an enzymatic colorimetry and an enzyme-linked immunosorbent assay technology. A technical principle of the ammonia (ammonia ion) content determination method comprises that determination is realized by a series of catalytic reactions of ammonia kinase, desthiobiotin synthetase and 6-methyl salicylic acid synthetase. The invention also relates to an ammonia (ammonia ion) diagnosis / determination kit. The ammonia (ammonia ion) content determination method provided by the invention has high sensitivity and a small error. The ammonia (ammonia ion) content determination method and the ammonia (ammonia ion) diagnosis / determination kit can be widely utilized for clinical medicine and food examination.

The invention relates to a determination method for determining ammonia (ammonium ion) content by utilizing an enzymatic colorimetric method and enzyme couple reaction technology, and composition and components of a reagent. The technology principle for the determination is that the determination is finished according to series catalytic reactions of ammonia kinase, biotin carboxylase, 6-methyl salicylate synthase. The invention further relates to a diagnosis / determination reagent kit of ammonia (an ammonium ion). The determination method is high in sensitivity and small in errors, and accordingly the determination method and the reagent kit can be widely applied to clinical medicine / food inspection.

The invention relates to a homocysteine diagnosing / measuring reagent (kit), which utilizes the technology of an enzymic colorimetric method and an enzyme coupled method. And the invention also relates to a method for measuring a concentration of homocysteine and compositions and components of the reagent and belongs to the technical field of the medical testing and measurement. The main components of the reagent (kit) of the invention comprise buffer solution, coenzyme, glutathione disulfide, coenzyme A-glutathione, 6-gaultherolin, sodium bicarbonate (carbon dioxide), glutathione homocysteine transhydrogenase, glutathione-coenzyme A-glutathione transhydrogenase, 6-methl salicylate synzyme and a stabilizing agent. The concentration of the homocysteine is measured by mixing a sample and the reagent in a certain volume ratio to make the sample and the reagent carry out a series of enzymatic reactions, arranging the reactant in an ultraviolet / visible light analyser and detecting the increasing degree of the absorbance at the 340nm position of the dominant wave.

The invention relates to a determination method of ammonia (ammonia ions) content by means of technologies of enzymatic colorimetry and enzyme-linked assay, and composition and components of a reagent. The technical principle of determination is based on a series of catalytic reactions of ammonia kinase, urea carboxylase and 6-methylsalicylic synthetase. The invention further relates to a diagnosis / determination kit for ammonia (ammonia ions). The determination method provided by the invention has high sensitivity and small error. Therefore, the determination method and the kit provided by the invention can be widely applied to clinical medical / food inspection.

The invention discloses a plant resistance related protein ANAC001, its encoding gene and an application thereof. The protein provided by the invention is named as ANAC001 protein, comes from Arabidopsis thaliana, and is any one of the following proteins: (a) a protein formed by an amino acid sequence as shown in the sequence 1; (b) a protein which is derived from the sequence 1 by replacing and / or deleting and / or adding one or more amino acid residues in the amino acid sequence of the sequence 1, and has any one of the following functions: a function of inducing ICS1 gene promoter, a function of inducing PR1 gene promoter, a function of being related with salicylic acid synthesis in plant and a function of being related with plant resistance. The ANAC001 protein provided by the inventioncan regulate and control the synthesis of salicylic acid in a plant so as to enhance plant disease resistance. The invention has a great value for the cultivation of disease resistant plants.

The invention relates to a one-step method for synthesizing salicylic acid with phenol and supercritical carbon dioxide, and relates to the technical field of salicylic acid synthesis. According to the method, supported potassium carbonate is adopted as a catalyst, and salicylic acid is synthesized with phenol and supercritical carbon dioxide. According to the invention, supported potassium carbonate is adopted as the catalyst, such that the product can be easily separated from the catalyst. A traditional process flow is simplified, and pickling and washing processes in the traditional processes are eliminated, such that the method is a green and clean production process. With the method, phenol conversion rate and salicylic acid selectivity are improved, and continuous production is realized. With the method, the phenol conversion rate can reach 100%, and the salicylic acid selectivity is up to 99.3%.

The invention discloses a plant resistance related protein ZIP11, its encoding gene and an application thereof. The protein provided by the invention is named as ZIP11 protein, comes from Arabidopsis thaliana, and is any one of the following proteins: (a) a protein formed by an amino acid sequence as shown in the sequence 1; (b) a protein which is derived from the sequence 1 by replacing and / or deleting and / or adding one or more amino acid residues in the amino acid sequence of the sequence 1, and has any one of the following functions: a function of inducing ICS1 gene promoter, a function ofinducing PR1 gene promoter, a function of being related with salicylic acid synthesis in plant and a function of being related with plant resistance. The ZIP11 protein provided by the invention can regulate and control the synthesis of salicylic acid in a plant so as to enhance plant disease resistance. The invention has a great value for the cultivation of disease resistant plants.