33 results about "Salicylic acid synthesis" patented technology

The invention discloses the method of improving the content of the salicin in the plant and the appropriative carrier. The carrier is the plant expressing carrier containing the separating offset acid composing enzyme gene and the separating offset acid-the pyruvic splitting dividing enzyme gene. The method uses the salicin composing approach and guides the different ICS gene and the IPL gene into the plant. The invention guides the said carrier into the plant group or the cell to gain the turning gene plant of the content of the salicin improved. The transferring gene test indicates that the dissociating state SA content in the transferring gene rice is mostly nine times than the wildness type. The method of the invention and the appropriative carrier can improve the content of the salicin in the plant greatly and provides the new method of amending the plant breed and improving the crop.

The invention discloses the method of improving the content of the salicin in the plant and the appropriative carrier. The carrier is the plant expressing carrier containing the separating offset acid composing enzyme gene and the separating offset acid-the pyruvic splitting dividing enzyme gene. The method uses the salicin composing approach and guides the different ICS gene and the IPL gene into the plant. The invention guides the said carrier into the plant group or the cell to gain the turning gene plant of the content of the salicin improved. The transferring gene test indicates that the dissociating state SA content in the transferring gene rice is mostly nine times than the wildness type. The method of the invention and the appropriative carrier can improve the content of the salicin in the plant greatly and provides the new method of amending the plant breed and improving the crop.

The present invention discloses an alkyl salicylic acid synthesis method, which comprises: mixing olefin with a carbon number of 4-50 and salicylic acid, and carrying out an alkylation reaction under a catalysis effect of aryl sulfonic acid at a temperature of 50-160 DEG C. According to the present invention, aryl sulfonic acid is adopted as a catalyst, a conversion rate can achieve 60-95%, the catalyst does not cause corrosion on the equipment, price is relatively low, the catalyst can be recycled, no three-waste emission problem exists, and environmental protection is easily achieved.

The invention discloses a plant resistance related protein ATSAR25, and encoding genes and application thereof. The protein provided in the invention is named ATSAR 25, originates from Arabidopsis thaliana, and is one selected from the following proteins: (a) a protein composed of an amino acid sequence represented by sequence 1; (b) a protein derived from sequence 1, wherein, the protein is obtained by substituting and / or deleting and / or adding one or more deoxynucleotide residue of the amino acid sequence of sequence 1 and has either the function of inducing promotion of ICS 1 gene promoters or the function of inducing promotion of PR 1 gene promoters; (c) a protein which is derived from sequence 1 and related to synthesis of salicylic acid in plants and to plant resistance. The ATSAR 25 protein provided in the invention can enhance disease resistance of plants and has a critical value to breeding of disease resistant plants.

An efficient synthesis method of salicylic acid belongs to the technical field of salicylic acid synthesis. The dehydration method adopted by the invention comprises the following steps: vacuumizing a sodium phenate solution for primary dehydration, adding phenol into the sodium phenate solution when the water content of the sodium phenate solution reaches about 45% such that a part of the phenol and water form a binary azeotrope, and separating the binary azeotrope under negative pressure condiction to achieve the purpose of taking out water by the phenol. The residual phenol and the sodium phenate form the bulky compound under the vacuum condition, and the bulky compound has the large specific surface area, such that the reaction rate is accelerated, the reaction time is substantially shortened, and the production efficiency is improved when the CO2 is introduced to perform the carboxylation reaction; meanwhile, the binary azeotrope and phenol separated in the process can be recycled, so that resources are saved, and the cost is reduced.

The invention discloses a plant resistance related protein MAG, and a coding gene and application thereof. The protein disclosed by the invention is named MAG protein, comes from Arabidopsis thaliana, and is (a) a protein composed of an amino acid sequence shown in sequence 1; or (b) a protein derived from sequence 1, which is formed by the substitution and / or deletion and / or addition of one or more amino acid residues in the amino acid sequence shown in sequence 1 and has any function of inducing the activation of an ICS1 gene promoter, inducing the activation of a PR1 gene promoter, being related to the synthesis of salicylic acid in plants or being related to the plant resistance. The MAG protein disclosed by the invention can enhance the disease resistance of plants by regulating and controlling the synthesis of salicylic acid in the plants. The invention has an important value for the cultivation of disease-resistant plants.

The invention relates to a method for synthesizing o-ethoxybenzoic acid from salicylic acid, which comprises the following steps: adding salicylic acid, acetone and potassium hydroxide into a reaction bulb, dropwisely adding bromoethane while stirring at room temperature, heating under reflux for 9-15 hours, recovering the acetone under reduced pressure, adding water and liquid alkali, heating under reflux for 2-4 hours, cooling to room temperature, regulating the pH value to 3-4 with glacial acetic acid, cooling to 1-15 DEG C, separating by vacuum filtration, washing with water, and carrying out vacuum drying to obtain the o-ethoxybenzoic acid, wherein the salicylic acid:potassium hydroxide:bromoethane:liquid alkali mol ratio is 1:(2.0-2.5):(2.1-2.5):1.5. The method has the advantages of simple technique, low raw material cost, high yield and good quality, and is convenient to operate and convenient for industrial production.

The invention discloses a Chinese medicine composition anti-wrinkle multi-function liquid, its preparation and application. The anti-wrinkle multi-function liquid comprises the following ingredients: 11-13 weight portions of the root of kudzu vine, 12-14 weight portions of rehmannia glutinosa, 7-9 weight portions of gingko, 11-13 weight portions of dark plum, 12-14 weight portions of leonurus, 4-6 weight portions of radix salvia miltiorrhizae, 6-8 weight portions of liquorice, 7-9 weight portions of Ligusticum wallichii, 6-8 weight portions of Angelica sinensis, 5-7 weight portions of Semen Cuscutae, 3-5 weight portions of glossy ganoderma, 4-6 weight portions of rhizoma cyperi, and 8-10 weight portions of ginseng. The multi-function liquid is light yellow and tasteless. The trade name ofthe multi-function liquid is aromlotus Chinese medicine composition anti-wrinkle multi-function liquid. The direct use of the multi-function liquid on the skin can effectively remove wrinkles, and can be absorbed by the skin effectively, so that the effects of skin care and skin beauty are achieved. According to the invention, no chemical additives are added in the multi-function liquid, and the multi-function liquid uses all natural extracts, so that the effects are realized depending on the Chinese medicine extracts completely; and the natural extracts are obtained by mixed extraction according to Chinese medicine formula, so that the characteristics of the compatibility of Chinese medicines are really shown.

The invention provides a preparation method of an aralkyl salicylic acid derivative. The method is characterized in that styrene or a derivative thereof reacts with salicylic acid or a derivative thereof in the presence of an ionic liquid catalyst. The method overcomes the disadvantages of present aralkyl salicylic acid synthesis technologies, and is an efficient and green aralkyl salicylic acid synthesis technology. The catalyst adopted in the invention settles to the bottom of a reactor after the reaction ends, is convenient to separate, and also has the advantages of multi-time repeated use, no emission of waste acids, and environment protection benefiting in case of guaranteeing good yield.

The invention discloses a plant resistance-related protein ATWRKY46 as well as an encoding gene and application thereof. The protein provided by the invention is named ATWRKY46 protein, which is from Arabidopsis thaliana and is any one of the following: (a) the protein formed by amino acid sequences as shown in a sequence 1; and (b) the protein which is derived from the sequence 1 in a way that the amino acid sequences as shown in the sequence 1 are subjected to replacement and / or deletion and / or addition of one or a plurality of amino acid residues, and has any function of the following: inducing a starting function of an ICS1 gene promoter, and inducing a starting function of a PR1 (pathogenesis-related protein) gene promoter, and the protein which is related to synthesis of salicylic acid in a plant and plant resistance and is derived from the sequence 1. The ATWRKY46 protein provided by the invention can increase disease resistance of the plant through regulating and controlling the synthesis of the salicylic acid in the plant. The invention is of great value to cultivate disease-resistant plants.

Disclosed is a novel supercritical phase salicylic acid synthesis process, which comprises making sodium phenolate through neutralizing phenol with NaOH aqueous solution, drying and grinding to obtain solid sodium phenolate, subjecting the sodium phenolate and carbon dioxide to supercritical homogeneous phase carboxylation reaction (Kolbe-Schmitt reaction) in heavy pressure reaction kettle, obtaining sodium salicylate, acidifying to prepare the salicylic acid.

The invention discloses a plant resistance related protein ANAC001, its encoding gene and an application thereof. The protein provided by the invention is named as ANAC001 protein, comes from Arabidopsis thaliana, and is any one of the following proteins: (a) a protein formed by an amino acid sequence as shown in the sequence 1; (b) a protein which is derived from the sequence 1 by replacing and / or deleting and / or adding one or more amino acid residues in the amino acid sequence of the sequence 1, and has any one of the following functions: a function of inducing ICS1 gene promoter, a function of inducing PR1 gene promoter, a function of being related with salicylic acid synthesis in plant and a function of being related with plant resistance. The ANAC001 protein provided by the inventioncan regulate and control the synthesis of salicylic acid in a plant so as to enhance plant disease resistance. The invention has a great value for the cultivation of disease resistant plants.

The invention relates to a one-step method for synthesizing salicylic acid with phenol and supercritical carbon dioxide, and relates to the technical field of salicylic acid synthesis. According to the method, supported potassium carbonate is adopted as a catalyst, and salicylic acid is synthesized with phenol and supercritical carbon dioxide. According to the invention, supported potassium carbonate is adopted as the catalyst, such that the product can be easily separated from the catalyst. A traditional process flow is simplified, and pickling and washing processes in the traditional processes are eliminated, such that the method is a green and clean production process. With the method, phenol conversion rate and salicylic acid selectivity are improved, and continuous production is realized. With the method, the phenol conversion rate can reach 100%, and the salicylic acid selectivity is up to 99.3%.

The invention discloses a plant resistance related protein ZIP11, its encoding gene and an application thereof. The protein provided by the invention is named as ZIP11 protein, comes from Arabidopsis thaliana, and is any one of the following proteins: (a) a protein formed by an amino acid sequence as shown in the sequence 1; (b) a protein which is derived from the sequence 1 by replacing and / or deleting and / or adding one or more amino acid residues in the amino acid sequence of the sequence 1, and has any one of the following functions: a function of inducing ICS1 gene promoter, a function ofinducing PR1 gene promoter, a function of being related with salicylic acid synthesis in plant and a function of being related with plant resistance. The ZIP11 protein provided by the invention can regulate and control the synthesis of salicylic acid in a plant so as to enhance plant disease resistance. The invention has a great value for the cultivation of disease resistant plants.