The invention provides a preparation method of a
cannabis sativa
protein ACE (
Angiotensin Converting Enzyme)
peptide inhibitor. The preparation method comprises the steps of A, weighing kernel
meal of degreased
cannabis sativa;
drying; crushing; sieving to remove impurities; feeding water until reaching fixed material-
liquid ratio; homogenizing through a
colloid mill; sieving; regulating the pH value; ultrasonically assisting to extract; extracting
protein with alkali under a constant temperature; performing isoelectric
precipitation; centrifuging to remove supernate; extracting
protein from precipitate with alkali for the second time; feeding water to reach the fixed material-
liquid ratio; homogenizing through a homogenizing
machine; sieving; regulating the pH value; performing isoelectric
precipitation again; centrifuging to remove supernate; neutralizing;
drying to obtain separated-out protein of kernel
meal of
cannabis sativa; B, feeding deionized water to separated-out protein of kernel
meal of cannabis satival; regulating pH; feeding mixed
protease for enzymolysis under the constant temperature; deactivating
enzyme by boiling water bath; centrifuging to obtain supernate; C, separating and purifying
protease enzymolysis solution of kernel meal of cannabis satival; collecting to obtain the ACE
peptide inhibitor of protein of kernel meal of cannabis satival; freezing and
drying to obtain the cannabis sativa protein ACE
peptide inhibitor. The method has the advantages that the operation is simple, the product yield is increased, and the
ACE inhibition effect is excellent.