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Preparation method of cannabis sativa protein ACE (Angiotensin Converting Enzyme) peptide inhibitor

A technology of hemp protein and inhibitory peptides, which is applied in the field of preparation of hemp protein ACE inhibitory peptides, can solve problems such as limitations and waste of resources, and achieve the effects of lowering blood pressure, improving utilization levels, and being easy to digest

Inactive Publication Date: 2015-04-01
AGRI PROD PROCESSING INST GUANGXI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In China, hemp seeds have always been pressed for oil, and the dregs are used as animal feed, resulting in a large waste of resources
With the further understanding of the nutritional function of hemp protein, existing enterprises and researchers have begun to use the hemp dregs after oil extraction for product processing, but most of them are limited to research on beverages and other products

Method used

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  • Preparation method of cannabis sativa protein ACE (Angiotensin Converting Enzyme) peptide inhibitor
  • Preparation method of cannabis sativa protein ACE (Angiotensin Converting Enzyme) peptide inhibitor
  • Preparation method of cannabis sativa protein ACE (Angiotensin Converting Enzyme) peptide inhibitor

Examples

Experimental program
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Effect test

Embodiment 1

[0028] 1. Extraction of hemp seed protein: extraction of hemp seed protein: take defatted hemp seed meal, dry, pulverize, and sieve to remove impurities. Add water until the ratio of material to liquid is 1:12g / mL, homogenate with a colloid mill, the temperature of the homogenate is 25°C, the gap between the colloid mill is 0.1mm, the homogenization time is 5min, the material after homogenization is passed through a 600 mesh sieve, and the The pH value was 8.5, and ultrasonic-assisted extraction was carried out at a temperature of 25°C, a power of 120W, and a duty ratio of 20:20s / s. Under the condition of a water bath temperature of 50°C, the protein was extracted for 60 minutes at a constant temperature, and then 1 mol / L of The HCl solution was adjusted to the isoelectric point of the hemp seed protein. After fully stirring, the protein was still precipitated to allow the protein to fully sink, and the supernatant was removed by centrifugation at 5000 r / min for 20 min; Add wa...

Embodiment 2

[0034]1. Extraction of hemp seed protein: take defatted hemp seed meal by weighing, dry, pulverize, and sieve to remove impurities. Add water until the material-to-liquid ratio is 1:20 g / mL, homogenate with a colloid mill, the homogenate temperature is 25°C, the gap between the colloid mill is 1mm, the homogenate time is 8min, and the material after homogenization is passed through a 600-mesh sieve. Adjust the pH value to 8, and carry out ultrasonic-assisted extraction at a temperature of 30°C, a power of 150W, and a duty ratio of 20:20s / s. Under the condition of a water bath temperature of 55°C, the protein was extracted by constant temperature alkali for 80 minutes, and then 1 mol / L The HCl solution was adjusted to the isoelectric point of the hemp seed protein, and after fully stirring, the static precipitation was allowed to fully sink the protein, and the supernatant was removed by centrifugation at 6000 r / min for 15 min; the precipitation was carried out for the second al...

Embodiment 3

[0040] 1. Extraction of hemp seed protein: take defatted hemp seed meal by weighing, dry, pulverize, and sieve to remove impurities. Add water until the ratio of material to liquid is 1:15 g / mL, homogenize with a colloid mill, the homogenization temperature is 30°C, the gap between the colloid mill is 0.5mm, the homogenization time is 10min, and the homogenized material is passed through a 600-mesh sieve , adjusted the pH value to 8, and carried out ultrasonic-assisted extraction at a temperature of 20°C, a power of 200W, and a duty ratio of 20:20s / s. Under the condition of a water bath temperature of 45°C, the protein was extracted by constant temperature alkali for 40 minutes, and then 1 mol / The HCl solution in 1 L was adjusted to the isoelectric point of the hemp seed protein. After fully stirring, the precipitation was static to allow the protein to fully sink, and the supernatant was removed by centrifugation at 6000 r / min for 15 minutes; the precipitation was carried ou...

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Abstract

The invention provides a preparation method of a cannabis sativa protein ACE (Angiotensin Converting Enzyme) peptide inhibitor. The preparation method comprises the steps of A, weighing kernel meal of degreased cannabis sativa; drying; crushing; sieving to remove impurities; feeding water until reaching fixed material-liquid ratio; homogenizing through a colloid mill; sieving; regulating the pH value; ultrasonically assisting to extract; extracting protein with alkali under a constant temperature; performing isoelectric precipitation; centrifuging to remove supernate; extracting protein from precipitate with alkali for the second time; feeding water to reach the fixed material-liquid ratio; homogenizing through a homogenizing machine; sieving; regulating the pH value; performing isoelectric precipitation again; centrifuging to remove supernate; neutralizing; drying to obtain separated-out protein of kernel meal of cannabis sativa; B, feeding deionized water to separated-out protein of kernel meal of cannabis satival; regulating pH; feeding mixed protease for enzymolysis under the constant temperature; deactivating enzyme by boiling water bath; centrifuging to obtain supernate; C, separating and purifying protease enzymolysis solution of kernel meal of cannabis satival; collecting to obtain the ACE peptide inhibitor of protein of kernel meal of cannabis satival; freezing and drying to obtain the cannabis sativa protein ACE peptide inhibitor. The method has the advantages that the operation is simple, the product yield is increased, and the ACE inhibition effect is excellent.

Description

technical field [0001] The invention belongs to the technical field of bioactive peptides, in particular to a preparation method of hemp protein ACE inhibitory peptide. Background technique [0002] At present, the research and utilization of hemp seed in China mainly focus on the extraction and utilization of hemp seed oil. Chronic diseases caused by the immune system and adjuvant therapy can help. In China, hemp seeds have been pressed for oil all the time, and the dregs are used as animal feed, resulting in a large waste of resources. With the further understanding of the nutritional function of hemp protein, existing enterprises and scientific researchers have begun to use the hemp dregs after oil extraction for product processing, but most of them are limited to research on beverages and other products. [0003] The content of protein in hemp seeds is about 25%, and contains 20 kinds of known amino acids, including 9 kinds of essential amino acids for human body. The...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/30C07K1/16
Inventor 游向荣张雅媛卫萍孙健李志春李明娟杨媚
Owner AGRI PROD PROCESSING INST GUANGXI ACADEMY OF AGRI SCI
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