The invention provides a high-accuracy quantitative detection kit for HIV-1
nucleic acid. The kit is applied to the field of biomedical
clinical diagnosis. The
gene amplification locus of the kit is positioned in the 5'LTR conserved region of an HIV
genome; the kit comprises a
nucleic acid extraction kit based on a
magnetic bead method and an HIV
nucleic acid amplification kit, wherein the nucleic acid extraction kit based on the
magnetic bead method comprises a lysed combined liquid, a rinsing liquid A, a rinsing liquid B, a rinsing liquid C, eluant and a
magnetic bead liquid; the HIV nucleic acid amplification kit comprises an RT-PCR reaction liquid, an
enzyme mixed liquid, an HIV-interior standard, an HIV quantitative
reference product 1-4, a negative
quality control product, a critical positive
quality control product and a strong positive
quality control product. The kit provided by the invention is simple and quick to operate, low in cost, high in detection sensitivity, good in
repeatability, high in primer and probe
gene type coverage rate, high in conservative property and strong in specificity, an efficient
internal standard system is introduced, and the problems of
mutual inhibition and interference due to the simultaneous amplification of a
target gene and the
internal standard are solved.