The invention discloses a fisheye camera calibration system, method and device, electronic equipment and a storage medium. The system comprises a hemispherical target, a fisheye camera and electronicequipment, wherein the hemispherical target comprises a hemispherical inner surface and a plurality of mark points arranged on the hemispherical inner surface; the fisheye camera is used for shootingthe hemispherical target and collecting a target image, and the target image comprises the hemispherical target and images of a plurality of mark points arranged on the hemispherical inner surface ofthe hemispherical target; the electronic equipment is used for obtaining initial values of k1, k2, k3, k4, k5, u0, v0, mu and mv, and Levenberg-Marquardt algorithm is used for optimizing initial values of k1, k2, k3, k4, k5, u0, v0, mu and mv to determine imaging model parameters of the fisheye camera. Through the hemispherical target, a plurality of target images are acquired without moving the target or a camera, and rapid and high-precision calibration of the fisheye camera can be realized only by acquiring one target image.