The invention discloses a method and device for multi-layer sampling of gas and water in a
drill hole. The method comprises the following steps of: A, putting a water suction pump and a packer in a specified layer position in the
drill hole; B, opening a
nitrogen cylinder, adjusting a pressure reducing valve, injecting
nitrogen into the packer through a setting
pipe, and realizing setting of the packer; C, switching on a power supply, and starting the water suction pump for sampling; D, after sampling, switching off the power supply, turning off the water suction pump, and releasing
nitrogen in the packer to realize deblocking of the packer; and E, lifting the packer and the water suction pump to the depth, and carrying out gas and water multi-layer sampling at the inner layer position ofthe
drill hole. The water suction pump is connected with the packer through a drill rod, a water suction pump cable penetrates through the packer and is connected with the power supply; the packer isconnected with the pressure reducing valve and the
nitrogen cylinder through the setting
pipe, the packer is connected with a gas and water separation device through the drill rod; an upper outlet isconnected with a gas sampling bag; and a lower outlet is connected with a
water sampling bottle. The device has the advantages of simple principle, clear structure, low cost, convenience in installation, simplicity and convenience in sampling, accurate and credible result and good application prospect.