30 results about "Callisia repens" patented technology

The invention discloses a roof greening method by using callisia repens, in particular to a growing method for planting callisia repens on roof. The procedures of the method are as follows: first, a water-resistant system, a drainage system and a root resistant layer are arranged on the roof and a matrix layer is paved on the roof; second, grass seeds of the callisia repens is planted on the matrix layer by using any one method of offshoots, broadcasting sowing method, cottage or greensward transplanting and paving method; third, root fixing water is watered and N fertilizer is fertilized once every fifteen days, and lawn is formed within one to two months. As an effective measure for solving the influence of urban development on the entironment and raising the urban afforestation rate, the invention uses the callisia repens on the roof greening, which has a resultful effect on the heat resistance, virescence and disaster prevention of room surface.

The invention discloses a taxus chinensis cuttage cultivation method. The taxus chinensis cuttage cultivation method comprises the steps as follows: inserting insertion branches treated with plant hormones onto a prepared seedbed substrate in sequence, controlling the cuttage depth to be 3-6 cm, watering each day, spraying a 700-time carbendazol solution once each week, after half a month, reducing the watering frequency, averagely watering once every 3 days, spraying the 700-time carbendazol solution once every two weeks, and transplanting for cultivating after the insertion branches take roots. The method has the advantages that firstly, as the seedbed substrate adopted in the method is better in physical permeability, formation, rooting and after-rooting growth of callus tissues are facilitated; secondly, saw powder is good in heat retaining property and the temperature difference between day and night for the substrate is slightly changed, so that rooting is facilitated; and thirdly, a small amount of humus soil is strong in water and fertility retaining capability, so that growth of root systems and new branches of nursery stocks is facilitated; the seedbed substrate is disinfected at the same time, so that the insertion branches are effectively prevented being infected with pathogenic bacteria; and the insertion branches are sprayed with the carbendazol solution after cuttage, so that the cuttage rooting is facilitated, the survival rate is increased, and the seedling growth time is shortened.

The invention relates to a culture medium formula of plant callus induction and subculture multiplication of astragalus membranaceus (fisch.) bunge and a culture method of the astragalus membranaceus (fisch.) bunge. Four steps of sterilizing selected explants, sterilizing a culture medium, inducing callus induction and carrying out callus subculture multiplication are included. A plant callus culture method of astragalus membranaceus (fisch.) bunge comprises the following steps: by taking astragalus membranaceus (fisch.) bunge leaves as the explants, adding a culture medium prepared from maltose, glucose, sucrose, agar powder, sodium alginate, naphthylacetic acid and 6-benzyl purine and the like into an MS basic culture medium to carry out tissue culture to obtain astragalus membranaceus (fisch.) bunge leaf callus; adjusting the pH value of the culture medium, and filling triangular culture bottles with a prepared callus induction culture medium and a prepared callus subculture multiplication culture medium respectively, and covering ventilated plastic sealing films on bottle openings; and sterilizing at the pressure of 0.10-0.15MPa and carrying out callus culture. According to the culture medium formula and the culture method, provided by the invention, the astragalus membranaceus (fisch.) bunge leaf calluses can be effectively induced and rapidly multiplied, and a lot of the astragalus membranaceus (fisch.) bunge leaf calluses can be obtained in relatively short time; and the astragalus membranaceus (fisch.) bunge leaf calluses have the characteristics of short production period and rapid multiplication speed and are suitable for industrial production.

The invention discloses an establishing method of a Gleditsia vestita Chun et Howex B.G.Li.tissue culture regeneration system. The rapid propagation system of Gleditsia vestita Chun et Howex B.G.Li.tissue culture is established by taking cotyledons of aseptic seedlings of Gleditsia vestita Chun et Howex B.G.Li. as explants through callus inducement, adventitious bud proliferation, rooting and acclimatizating and transplanting. The results show that: the cotyledons of the Gleditsia vestita Chun et Howex B.G.Li.simultaneously induce callus and buds on a MS+6-BA3.0mg/L+KT0.4mg/L+IAA0.3mg/L culture medium, and the average induction rate reaches 90.5%; the best culture medium of adventitious bud proliferation is 1/2MS+6-BA1.0mg/L +IBA0.3mg/L+NAA0.2mg/L, and the proliferation multiple is 4.0; under the condition of MS+NAA0.5mg/L+IAA0.25mg/L, the rooting rate is up to 100%; plants after transplanting are good in growth vigor, and the survival rate is over 90%.

The invention provides a seedling cultivation method for kiwi fruit. The seedling cultivation method comprises the following steps of: grafting which is performed from the end of December to next January; sand storage, namely, seedlings subjected to the grafting are stored by river sand indoors; and planting of the seedlings in a nursery garden from February to the beginning of March. According to the invention, the grafting is performed in winter to avoid busy farming seasons of spring and summer, and the grafting time can be reasonably scheduled at daytime or at night as the grafting is performed indoors. After the seedlings subjected to the grafting are stored by the river sand indoors, most of the seedlings form callus at graft unions when being transplanted since an indoor temperature is high, thereby being beneficial to improving the rate of survival of the grafted seedlings. By virtue of the seedling cultivation method, the rate of survival of the grafted seedlings can reach 99%; and the pruning of stocks in a bleeding period is avoided, thus the nutrient loss of the stocks is reduced.

The invention discloses a method for inducing calluses of peony anther. A murashige and skoog (MS) culture medium is adopted to induce the calluses; and substances such as 6-benzylamino adenine (BA) cytokinin, a naphthylacetic acid (NAA) plant growth regulator, a 2,4-D herbicide and the like are prepared in the MS culture medium. The definite culture medium scheme and hormone combination are provided for a peony anther explant. The optimal material taking period of the calluses is presented. The inductivity of peony calluses is over 80 percent, and the better induction effect is achieved; and the inductivity of the calluses of the anther is over 50 percent.

The invention discloses a method for arranging a greening layer on the top of a building or a public facility. The method comprises the step of laying a callisia repens lawn with soil or callisia repens blanket without soil on the top of the building or the public facility. Callisia repens is laid on the top of the building or public facility and can be used as a house thermal insulation layer for protecting the building and prolonging the service life of the building; stagnant water drainage load can be lightened, the urban water environment is improved; and urban air can be purified, the noise is lowered, the automobile exhaust is filtered, and the pollution of PM2.5 is lightened.

A disclosed method for utilizing lilium brownie roots to induce callus and embryoid comprises the following steps: 1) roots of lilium brownie aseptic seedling are selected and inoculated to an induction medium for dark culture with the culture room temperature of 23-27 DEG C; 2) embryoids on callus obtained by dark culture are transplanted to a growth rooting medium for culture in light with illumination for 16-20 h every day at a temperature of 23-27 DEG C; and 3) the embryoids are greened, gradually grow into normal plants, and root. By employing the specific culture method and the culture medium provided by the invention, the inductivity of callus induced by lilium brownie roots reaches 100%, all root explants are overgrown with calluses after cultured for about 50 days, the embryoid inductivity is 90% and the plants grow well.

The invention discloses a method for inducing callus of peony petals, applying an MS culture medium that contains 6-BA cytokinin, NAA plant growth regulator, 2, 4-D weedicide and the like to induce callus. Specific culture medium scheme and hormone combination are supplied for explants of peony petals. The optimal material drawn period of the callus is described in the invention. The inductivity of the peony callus is more than 80% and a preferable induction effect is achieved; and the inductivity of the petal callus is higher than 50%.

The invention discloses a rapid propagation method for sapium sebiferum callus seedlings. The method comprises the following steps: 1, taking sapium sebiferum seeds, removing seed coat, disinfecting to obtain explants, inoculating the explants into an MS germination culture medium, and culturing for several days to obtain sterile test-tube plantlets; 2, placing cotyledons of the sterile test-tubeplantlets in a WPM callus culture medium, culturing to obtain callus, culturing the callus in a WPM differential culture medium to obtain multiple shoots, culturing the multiple shoots in a WPM soundseedling culture medium to obtain plants, and culturing the plants in a 1/2 WPM rooting culture medium, thereby obtaining rooted seedlings. According to the method disclosed by the invention, lots ofsapium sebiferum seedlings suitable for transplanting are cultivated in a short time, the multiplication coefficient and seedling quality of the sapium sebiferum seedlings are obviously improved, andthe requirements on the sapium sebiferum seedlings in the market are met.

The invention relates to a southern vegetable greenhouse roof green grass patch and a production method thereof. The green grass paste comprises a substrate layer, mosses, carbonized garden wastes andcallisia repens; the substrate layer comprises the mosses and the carbonized garden wastes with a weight percentage of 1:1, the mosses and the carbonized garden wastes are evenly mixed, and then pressed to form 1-1.5 cm thick sheet patches; and callisia repens is planted on the substrate. The production method is as follows: step 1: collecting fresh mosses, performing washing and cleaning, and performing drying to make the water content of the mosses to be 15-20%; step 2: crushing dead branches in gardens to be 20-40 mesh, and performing carbonizing; step 3: mixing the mosses in step 1 and the carbonized garden wastes in step 2 according to the ratio of 1:1, and pressing the mixture into 1-1.5 cm thick sheets to form the substrate; and step 4: planting callisia repens on the substrate prepared in step 3, and then covering southern vegetable greenhouse roofs.

The invention discloses a method for induction of a panax notoginseng purpurin callus. Tender stems, leaves, flowers or anthers of panax notoginseng plants are used as explant, and sterilization, inoculation and induction culture are conducted on the explant to obtain the panax notoginseng purpurin callus; 4-8 h of illumination and 4-8 h of dark treatment are conducted alternately for induction culture; a culture medium for induction culture is MS+0.1-2 mg/L 2,4-D+0.1-2 mg/L BA+0.002-1 mg/L TDZ+30-60 g/L saccharose or MS+0.1-2 mg/L NAA+0.1-2 mg/L BA+0.002-1 mg/L TDZ+30-60 g/L saccharose. By controlling illumination time, the culture medium, moisture, temperature and other conditions, the callus rich in purpurin and high in inductivity is cultured. By shortening the alternation time of illumination and dark treatment, the content of panax notoginseng purpurin is effectively increased; by adding TDZ to the culture medium to be matched with other plant hormones, the inductivity of the panax notoginseng callus is improved remarkably, and formation of purpurin can be achieved easily. The method lays a foundation for mass extraction of purpurin in the panax notoginseng callus.