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Ranunculus chinensis callus culture method

A technology of callus and tissue culture, applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effects of fast value-added, large output, and low energy consumption

Inactive Publication Date: 2014-05-21
程玉鹏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a method for culturing the callus of fennel, which can obtain a large amount of callus of fennel in a relatively short period of time, and solve the problem of resource demand of fennel plants by means of biotechnology. Active ingredients of fennel provide sufficient raw materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] (1) Selection and sterilization of explants: the stems of fennel garlic were used as explants. Collect healthy and disease-free fennel garlic stems, wash them three times with sterile water, soak them in 75% ethanol for 40 seconds, and then put them in 0.1% HgCl 2 Soak in the solution for 10min, then wash with sterile water 3 times.

[0011] (2) Callus induction: the sterilized fennel stems were cut into 8mm-long sections with a sterile scalpel, and inoculated on the callus induction medium. The formula of the callus induction medium is: MS+6-BA0.5mg / L+NAA0.3mg / L+sucrose 30g / L+agar 6g / L, and the pH value is 5.8. The culture temperature is 25°C, the light is 12 hours a day, and the light intensity is 2000lx. After 30 days of culture, the callus induction rate was 90%.

[0012] (3) Callus expansion and propagation: under sterile conditions, select green or light yellow callus without browning and compact texture, cut it into cubes with a side length of 5mm, and transfe...

Embodiment 2

[0014] (1) Selection and sterilization of explants: the stems of fennel garlic were used as explants. Collect healthy and disease-free fennel garlic stems, wash them three times with sterile water, soak them in 70% alcohol for 45 seconds, then soak them in 0.15% HgCl2 solution for 5 minutes, and then wash them three times with sterile water.

[0015] (2) Callus induction: the sterilized fennel stems were cut into 10 mm length segments with a sterile scalpel, and inoculated on the callus induction medium. The formula of the callus induction medium is: MS+6-BA1mg / L+NAA0.1mg / L+sucrose 20g / L+agar 5g / L, and the pH value is 5.8. Culture temperature is 30 ℃ , 6 hours of light per day, light intensity 3000lx. After 30 days of culture, the callus induction rate was 80%.

[0016] (3) Callus expansion and propagation: Under sterile conditions, select green or light yellow callus without browning and compact texture, cut it into cubes with a side length of 8 mm, and transfer it to call...

Embodiment 3

[0018] (1) Selection and sterilization of explants: the stems of fennel garlic were used as explants. Collect healthy and disease-free fennel garlic stems, wash them three times with sterile water, soak them in 75% ethanol for 25 seconds, and then put them in 0.1% HgCl 2 Soak in the solution for 15 minutes, and then wash 3 times with sterile water.

[0019] (2) Callus induction: the sterilized fennel stems were cut into 5mm-long sections with a sterile scalpel, and inoculated on the callus induction medium. The formula of the callus induction medium is: MS+6-BA0.3mg / L+NAA1mg / L+sucrose 40g / L+agar 8g / L, and the pH value is 6.0. The culture temperature is 20°C, the light is 16h per day, and the light intensity is 1000lx. After 30 days of culture, the callus induction rate was 84%.

[0020] (3) Callus expansion and propagation: Under sterile conditions, select green or light yellow callus without browning and compact texture, cut it into cubes with a side length of 3 mm, and tr...

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Abstract

The invention relates to a plant callus culture method and particularly relates to a ranunculus chinensis callus culture method. The ranunculus chinensis callus culture method comprises the following three steps: selecting explants and sterilizing; inducing calluses; amplifying and reproducing the calluses. According to the ranunculus chinensis callus culture method, a lot of the ranunculus chinensis calluses can be obtained in short time, and the effective induction and rapid reproduction of the ranunculus chinensis calluses are realized; the demanding problem of ranunculus chinensis resources is solved; the ranunculus chinensis callus culture method has the characteristics of short production period and rapid reproduction speed, and can be used for extraction and pharmaceuticals industries of effective components of ranunculus chinensis.

Description

technical field [0001] The invention relates to a plant callus culture method, in particular to a fennel garlic callus culture method. Background technique [0002] Fennel garlic (Ranunculus chinensis Bunge) is a plant of Ranunculaceae (Ranunculaceae) and has high medicinal value. The whole herb is used as medicine with a wide range of medicinal uses, such as anti-inflammatory, analgesic, anti-malarial, and insecticidal effects. It is often used to treat diseases such as hepatitis, cirrhosis, malaria, gastritis, ulcer asthma, and rheumatic joint pain. At present, although fennel garlic has been used clinically, its plant resources basically come from wild excavation, and it is difficult to form large-scale production. damage. The use of tissue culture technology to produce medicinal plants has the characteristics of short growth cycle, high reproduction rate, and annual cultivation without seasonal restrictions. At present, there is no research report on the large-scale p...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 程玉鹏高宁刘博
Owner 程玉鹏
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