A method for hardening and transplanting of callus-induced T.

Abstract

The invention provides a method for hardening and transplanting callus-induced tissue-cultured seedlings of S. martinata, belonging to the technical field of plant tissue culture. The seedling hardening and transplanting method of the callus-induced P. chinensis tissue-cultured seedlings provided by the present invention comprises: taking the callus-induced P. chinensis tissue-cultured seedlings to harden the seedlings for 5 to 10 days to obtain the hardened seedlings, said The temperature for seedling hardening is 15-18°C, the light intensity is 800-1000lx, the light is 5-8h per day, and the seedling hardening humidity is 70-80%; the obtained seedlings after hardening are transplanted into the transplanting medium, Add the nutrient solution to the substrate at the roots of the seedlings. Through the seedling hardening and transplanting process of the present invention, the transplanting survival rate of the callus-induced B. The problem of low survival rate after transplantation of tissue cultured seedlings induced by the callus of Martin chrysanthemum.

Description

technical field [0001] The invention belongs to the technical field of biological tissue culture, and more specifically relates to a method for hardening and transplanting callus-induced callus tissue-cultured seedlings. Background technique [0002] Syringareticulata (Blume), also known as Paomazi and white lilac, is a small deciduous tree of the clove tree in the family Oleaceae. There are about 35 species of lilac trees in the world, mainly distributed in the temperate regions of Asia and Europe, and more than 25 species are produced in China. As an ornamental plant, it is one of the most commonly used tree species in gardens in northern my country because of its luxuriant branches and leaves, large inflorescences, and strong fragrance. In addition, its material is solid and dense, and its structure is uniform; its essential oil content is rich and has a wide range of properties. biological activity; the landscape value and economic value of S. martinica have been paid mo...

AI Technical Summary

Problems solved by technology

However, the seeds used for seed propagation require a complicated germination process, and the emergence of seedlings is uneven, the initial growth is slow, and the fruit is affected by the size of the year, which makes lilac varieties more flower-seeing types, because most of them are heterozygous genetic types , so seed propagation cannot maintain the typical traits of its variety

[0003] At present, some studies have been done on the in vitro culture of Matinaceae, and a relatively systematic tissue culture regeneration system of Matinaceae has been established by using two methods of bud proliferation and callus tissue. Under the cultivation environment, the root system adapts to the surrounding environment with sufficient water and nutrients. Secondly, the test-tube seedlings are small, have very limited buffering capacity against adverse environments, and have poor resistance. They are directly transplanted into the substrate and placed in the natural environment. It is very easy to cause water shortage and wilt in the environment, which reduces the survival rate; in addition, for P. martinica, the tissue cultured seedlings induced by callus are weaker than those induced by general stem segments, which aggravates the transplanting mortality and makes the lower survival rate