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Method for inducing calluses of peony anther

A callus induction and callus technology, applied in the field of callus culture, can solve the problems of difficulty in domestication and transplanting, low rooting rate and rooting quality, browning, etc., and achieve the effect of high callus induction rate

Inactive Publication Date: 2011-08-10
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0008] There are problems such as difficulty in callus induction, low rooting rate and quality, difficulty in acclimatization and transplanting, browning, vitrification, etc.

Method used

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Experimental program
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specific Embodiment

[0019] Taking different explants as an example, the best induction effect was achieved by using different media and different culture conditions. In the following, different explants are used as objects to elaborate respectively:

[0020] Induction of embryonic callus:

[0021] Cultivation period: every September, after the seeds are formed;

[0022] Pre-cultivation treatment: use low temperature (4°C) treatment and gibberellin treatment combined method, use conventional disinfection methods (2% NaCl0 disinfection for 30 minutes, 70% alcohol disinfection for 30 seconds, rinse with sterile water for 3-5 times) after disinfection vaccination;

[0023] The best medium: MS+NAA0.1mg / L+6-BA1.0mg / L;

[0024] The callus induction rate reached 100%.

[0025] Stem segment callus induction:

[0026] Material collection period: take young stems, in late March and early April in spring, or young stems formed by germination of seeds;

[0027] Pre-cultivation treatment: use conventiona...

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Abstract

The invention discloses a method for inducing calluses of peony anther. A murashige and skoog (MS) culture medium is adopted to induce the calluses; and substances such as 6-benzylamino adenine (BA) cytokinin, a naphthylacetic acid (NAA) plant growth regulator, a 2,4-D herbicide and the like are prepared in the MS culture medium. The definite culture medium scheme and hormone combination are provided for a peony anther explant. The optimal material taking period of the calluses is presented. The inductivity of peony calluses is over 80 percent, and the better induction effect is achieved; and the inductivity of the calluses of the anther is over 50 percent.

Description

[0001] This application is a divisional application of: [0002] Application number: 200910083744.3; Invention name: method for inducing peony callus; application date: 2009.5.11. technical field [0003] The invention relates to a callus culture method, in particular to a peony anther callus induction method. Background technique [0004] Peony is one of the top ten famous flowers in China, and it is also the national flower of China. It has the reputation of "the king of flowers". Tissue culture of tree peony mainly uses buds to induce plants and explants to induce plants through callus, and the callus induction of tree peony is an important stage for plant regeneration, and a higher callus induction rate is more important for later callus. How to obtain a higher callus induction rate is closely related to the type of medium, the type and concentration of hormones, and the culture conditions. [0005] Callus culture is the most common form of culture. Except for shoot ti...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王雁朱向涛
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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