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Eucommia ulmoides Oliv. in-vitro rapid-propagation method

An in vitro and rapid technology, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of slow in vitro reproduction speed, low efficiency, and low utilization efficiency of in vitro materials, and achieve fast reproduction speed and high efficiency. High, the effect of improving utilization efficiency

Active Publication Date: 2015-05-13
镇巴县泰昌农牧有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the existing methods have the following problems: the utilization efficiency of in vitro materials is not high, and the speed of in vitro reproduction is slow and the efficiency is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1 uses adventitious roots formed by cotyledons as materials

[0020] 1. Obtain adventitious roots: The fruits of Eucommia ulmoides were brewed in flowing water for 48 hours, peeled off the peel, cleaned and disinfected in an ultra-clean workbench, and then the seed embryos were peeled off, inoculated on MS basic medium, and cultured for two weeks. Obtain sterile seedlings. Cut the cotyledons of the seedlings, and cut the cotyledons into segments of about 5 mm; the cotyledon segments are placed horizontally on the surface of the MS+naphthalene acetic acid (abbreviated as NAA, which is a kind of auxin) medium, and each liter of MS medium contains 0.2mg NAA, press gently to make the cotyledons fully contact with the medium; adventitious roots begin to form after 2 weeks, and the white adventitious roots can reach up to 6-10cm in length around 8 weeks.

[0021] 2. Induce the formation of adventitious buds: Cut the formed adventitious roots into 1 cm segments and...

Embodiment 2

[0025] Embodiment 2 uses the adventitious root formed by callus as material

[0026] 1. Obtain adventitious roots: inoculate the callus on the surface of the medium of 1 / 2MS+indoleacetic acid (abbreviated as IAA, which is a kind of auxin), each liter of medium contains 1mg IAA, and press gently to make the callus The tissue is in full contact with the medium; adventitious roots begin to form after 1 week, and the longest adventitious root can reach 6-8 cm in about 7 weeks.

[0027] 2. Induce the formation of adventitious buds: Cut the formed adventitious roots into 1 cm segments and inoculate them on MS medium containing plant growth regulators. The plant growth regulator is TDZ, containing 2 mg TDZ per liter of MS medium. At the same time, 0.1 mg of NAA was added per liter of MS medium. After about 10 days of culture, callus began to form at both ends of the cut segment, and gradually expanded to the middle. About 30 days later, the cut segments formed adventitious buds. ...

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Abstract

The invention discloses an Eucommia ulmoides Oliv. in-vitro rapid-propagation method. The Eucommia ulmoides Oliv. in-vitro rapid-propagation method is characterized by comprising the following steps of culturing explants by an MS medium containing an auxin or an auxin-like substance so that adventitious root is formed, cutting the adventitious root into sections, inoculating an MS medium containing a plant growth conditioning agent with the sections so that adventitious buds are formed, culturing the adventitious buds with mature leaves by a 1 / 2 MS medium containing the auxin-like substance for induced rooting so that test-tube seedlings with roots and functional leaves are formed, naturalizing the test-tube seedlings, and carrying out transplanting colonization. Through a plant in-vitro breeding technology, adventitious root formed from multiple explants such as in-vitro cultured Eucommia ulmoides Oliv. cotyledon and callus is used as a raw material, the adventitious buds are obtained efficiently by induction, and the test-tube seedlings with roots are transplanted in the field so that a clonal line is obtained; and the method provides a novel approach for Eucommia ulmoides Oliv. in-vitro rapid-propagation, has a high propagation rate and high efficiency, and improves Eucommia ulmoides Oliv. detached material utilization efficiency.

Description

technical field [0001] The invention relates to a propagation method for plant seedlings, in particular to a method for rapid propagation of Eucommia ulmoides in vitro, and belongs to the technical field of asexual propagation. Background technique [0002] Eucommia ulmoides Oliv. is a unique tertiary relict and precious medicinal tree species in my country. Its bark is the traditional precious traditional Chinese medicine - Eucommia ulmoides, which is a national second-class protected plant. At present, Eucommia ulmoides is mainly propagated by seeds, but the germination rate of seeds is not high. In fact, there are large differences between individual progeny, and the content of effective medicinal components and Eucommia gum varies significantly, which is not conducive to the promotion of newly bred Eucommia superior lines. [0003] Plant in vitro culture technology, tissue culture technology, and rapid in vitro propagation can not only shorten the breeding time, but also...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 曹小勇秦公伟蒋景龙徐皓刘艳丽赵桦李新生
Owner 镇巴县泰昌农牧有限公司
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