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Rapid propagation method for melia toosendan tissue culture

A technology of tissue culture and Toosendan, which is applied in the field of plants, can solve the problems that tissue culture technology has not been reported yet, and achieve the effects of large-scale planting, large output and short cycle

Inactive Publication Date: 2014-12-24
NANJING DIDAO AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, it is also an important raw material for making high-efficiency, non-residual, toxic and pollution-free new plant pesticides. Toosendan is bitter and cold, mainly enters the liver meridian, relieves liver heat, promotes qi and relieves pain, and treats qi stagnation. At present, it is mainly propagated by seeds. Planting methods, tissue culture techniques have not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Take the young leaves of Toosendan, wash them with running water for 13 minutes, disinfect them with 0.1% mercuric chloride on the ultra-clean workbench for 8 minutes, wash them with sterile water 6 times, insert the sterilized young leaves of Toosendan into BL+ZT1.5mg / L+2, 4-D 0.2mg / L+15% loofah wound fluid, in induction medium supplemented with 0.7% agar and 30g / L sucrose, pH 5.8, cultured in dark light, temperature 25±2℃, induced Put the callus into the medium BL+NAA1.4mg / L+ZT2.5mg / L+10% loofah wound liquid for callus differentiation, add sucrose 30g / L, agar 7g / L, pH5.8, light 2800lx, temperature 25±2°C, put the differentiated sprouts into 1 / 3MS+300ppm rooting powder medium for rooting induction, add 30g / L sucrose, 7g / L agar, pH5.8, light 6000lx, temperature 25± Put the rooted Toosendan in the field soil: vermiculite: sawdust = 3:1:1 at 2°C, use automatic water spraying device, spray 90ppm NAA solution once every three days, and spray 600 times more every two weeks ...

Embodiment 2

[0011] Take the young leaves of Toosendan, wash them with running water for 13 minutes, disinfect them with 0.1% mercuric chloride on the ultra-clean workbench for 8 minutes, wash them with sterile water 6 times, insert the sterilized young leaves of Toosendan into BL+ZT1.5mg / L+2, 4-D 0.2mg / L+15% loofah wound fluid, in induction medium supplemented with 0.7% agar and 30g / L sucrose, pH 5.8, cultured in dark light, temperature 25±2℃, induced Put the callus into the medium BL+NAA1.4mg / L+ZT2.5mg / L+20% loofah wound liquid for callus differentiation, add sucrose 30g / L, agar 7g / L, pH5.8, light 2800lx, temperature 25±2°C, put the differentiated sprouts into 1 / 3MS+400ppm rooting powder medium for rooting induction, add 30g / L sucrose, 7g / L agar, pH5.8, light 6000lx, temperature 25± Put the rooted Toosendan in the field soil: vermiculite: sawdust = 3:1:1 at 2°C, use automatic water spraying device, spray 90ppm NAA solution once every three days, and spray 600 times more every two weeks ...

Embodiment 3

[0013] Take the young leaves of Toosendan, wash them with running water for 13 minutes, disinfect them with 0.1% mercuric chloride on the ultra-clean workbench for 8 minutes, wash them with sterile water 6 times, insert the sterilized young leaves of Toosendan into BL+ZT1.5mg / L+2, 4-D 0.2mg / L+15% loofah wound fluid, in induction medium supplemented with 0.7% agar and 30g / L sucrose, pH 5.8, cultured in dark light, temperature 25±2℃, induced Put the callus into the medium BL+NAA1.4mg / L+ZT2.5mg / L+20% loofah wound liquid for callus differentiation, add sucrose 30g / L, agar 7g / L, pH5.8, light 2800lx, temperature 25±2°C, put the differentiated sprouts into 1 / 3MS+300ppm rooting powder medium for rooting induction, add 30g / L sucrose, 7g / L agar, pH5.8, light 6000lx, temperature 25± Put the rooted Toosendan in the field soil: vermiculite: sawdust = 3:1:1 at 2°C, use automatic water spraying device, spray 90ppm NAA solution once every three days, and spray 600 times more every two weeks ...

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Abstract

The invention provides a rapid propagation method for melia toosendan tissue culture. The rapid propagation method for melia toosendan tissue culture comprises the steps of obtaining aseptic melia toosendan leaves, inducing callus tissues, differentiating the callus tissues, inducing rooting, performing acclimatization and transplanting and the like. By means of the rapid propagation method for melia toosendan tissue culture, high-quality seedlings can be cultivated, a cultivation period can be shortened, genetic resources can be preserved, and a basis is provided for melia toosendan development and utilization.

Description

technical field [0001] The invention relates to a rapid propagation method for Toosendan tissue culture, belonging to the technical field of plants. Background technique [0002] Toosendan, Melia toosendan , also known as Jinlingzi, Toosendan, a traditional Chinese medicine, is the mature fruit of the deciduous tree of the Meliaceae family. Produced in China's Gansu, Hubei, Sichuan, Guizhou and Yunnan provinces, and widely cultivated in other provinces and regions; born in wet and fertile miscellaneous forests and sparse forests, especially those produced in Sichuan, distributed in Japan and Indochina Peninsula. Toosendan is cold in nature and bitter in taste. It is a kind of qi-promoting medicine. It likes warm and humid climate, likes sun, and is not tolerant to shade. It can grow at altitudes below 1100m. It has the effects of soothing the liver, promoting qi and relieving pain, and deworming. It is used for distending pain in the chest and flanks, abdominal distention,...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 刘东锋杨成东
Owner NANJING DIDAO AGRI SCI & TECH
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