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Rapid propagation method for sapium sebiferum callus seedlings

A callus and tallow technology, applied in the field of plant reproduction, can solve problems such as slow propagation of tallow, achieve the effects of improving the quality of seedlings, increasing the multiplication coefficient, and realizing large-scale production

Inactive Publication Date: 2018-03-09
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the conventional propagation of Chinese tallow tree is slow, which is far from meeting the market demand for Chinese tallow tree and its extracts. Therefore, it is imminent to carry out industrial seedling cultivation of Chinese tallow tree through artificial cultivation, breeding of fine varieties, and tissue culture.

Method used

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  • Rapid propagation method for sapium sebiferum callus seedlings
  • Rapid propagation method for sapium sebiferum callus seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of rapid propagation method of Chinese tallow tree callus into seedling, comprises the following steps:

[0023] Step 1: Take Chinese tallow tree seeds, remove the seed coats, soak them in 2% detergent solution for 5 minutes, rinse them with tap water for 15 minutes, soak them in 50% 84 disinfectant for 5 minutes for disinfection, and obtain explants.

[0024] Step 2. Inoculate the explants into the MS germination medium, and cultivate them for 20 days at a temperature of 22° C., a light intensity of 1500 lux, and a light time of 8 h / d. After the seeds germinate, sterile test-tube plantlets are obtained. 1 liter of MS germination medium contained 0.1 mg of GA3 gibberellin, 30 mg of sucrose and 5 mg of agar, and the pH of the MS germination medium was 5.8.

[0025] Step 3: Inoculate the cotyledons of sterile test-tube plantlets on the surface of WPM callus medium, and cultivate them for 30 days at a temperature of 22° C. in the dark to obtain callus tissue, wherei...

Embodiment 2

[0031] A kind of rapid propagation method of Chinese tallow tree callus into seedling, comprises the following steps:

[0032] Step 1: Take Chinese tallow tree seeds, remove the seed coat, soak them in 2% aqueous solution of detergent for 5 minutes, wash the linear tap water for 20 minutes, soak in 50% 84 disinfectant for 8 minutes for disinfection treatment, and obtain explants.

[0033] Step 2. Inoculate the explants into the MS germination medium, and cultivate them for 20 days at a temperature of 26° C., a light intensity of 1500 lux, and a light time of 10 h / d. After the seeds germinate, sterile test-tube plantlets are obtained. 1 liter of MS germination medium contained 0.5 mg of GA3 gibberellin, 30 mg of sucrose and 5 mg of agar, and the pH of the MS germination medium was 5.8.

[0034] Step 3: Inoculate the cotyledons of sterile test-tube plantlets on the surface of WPM callus medium, and cultivate them for 30 days at a temperature of 26° C. in the dark to obtain callu...

Embodiment 3

[0040] A kind of rapid propagation method of Chinese tallow tree callus into seedling, comprises the following steps:

[0041] Step 1: Take Chinese tallow tree seeds, remove the seed coat, soak them in 2% detergent solution for 5 minutes, rinse them with tap water for 18 minutes, soak them in 50% 84 disinfectant for 7 minutes for disinfection, and obtain explants.

[0042] Step 2. Inoculate the explants into the MS germination medium, and cultivate them for 20 days at a temperature of 24° C., a light intensity of 1500 lux, and a light time of 9 h / d. After the seeds germinate, sterile test-tube plantlets are obtained. 0.3 mg of GA3 gibberellin, 30 mg of sucrose and 5 mg of agar were contained in 1 liter of MS germination medium, and the pH of the MS germination medium was 5.8.

[0043] Step 3: Inoculate the cotyledons of sterile test-tube plantlets on the surface of the WPM callus medium, and cultivate them for 30 days at a temperature of 24° C. in the dark to obtain callus tis...

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Abstract

The invention discloses a rapid propagation method for sapium sebiferum callus seedlings. The method comprises the following steps: 1, taking sapium sebiferum seeds, removing seed coat, disinfecting to obtain explants, inoculating the explants into an MS germination culture medium, and culturing for several days to obtain sterile test-tube plantlets; 2, placing cotyledons of the sterile test-tubeplantlets in a WPM callus culture medium, culturing to obtain callus, culturing the callus in a WPM differential culture medium to obtain multiple shoots, culturing the multiple shoots in a WPM soundseedling culture medium to obtain plants, and culturing the plants in a 1 / 2 WPM rooting culture medium, thereby obtaining rooted seedlings. According to the method disclosed by the invention, lots ofsapium sebiferum seedlings suitable for transplanting are cultivated in a short time, the multiplication coefficient and seedling quality of the sapium sebiferum seedlings are obviously improved, andthe requirements on the sapium sebiferum seedlings in the market are met.

Description

technical field [0001] The invention relates to the technical field of plant propagation. More specifically, the present invention relates to a method for rapid propagation of Chinese tallow tree callus into seedlings. Background technique [0002] Sapium sebiferum Roxb (Sapium sebiferum Roxb) is a plant of the genus Sapium genus Euphorbiaceae, also known as wax tree, wood oil tree, wood wax tree, deciduous small tree. Chinese tallow tree has a wide range of uses. The seeds can be processed into catalpa oil. It is a famous industrial oil tree species in China. The oil yield is as high as 40%. It is used to make soap, wax paper, skin care cream, metal liniment, solid alcohol and high-grade spices, Stearic acid, etc., so people call tallow forest a green oil depot. Sapium cake can be used as fuel and fodder, and the husk can extract potassium carbonate. The leaves and bark of the stems contain tannin, and the leaves are poisonous. They can kill insects, raise tallow silkwor...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李刚徐函兵王晓峰唐美琼李正文
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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