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Anti-browning culture medium for elaeagnus mollis tissues and preparation method of anti-browning tissue culture medium

A technology for oil tree and culture medium, which is applied in the field of anti-browning medium of oil tree tissue and its preparation, can solve the problems of browning of tissue cells, low rooting rate and the like, and achieves improved production, improved rooting rate and The effect of rooting number, good economic benefit and social benefit

Active Publication Date: 2019-06-28
西安同人农林实业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this medium does not effectively solve the browning problem of tissue cells, and the rooting rate is low, the maximum rooting rate is 36.7%

Method used

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  • Anti-browning culture medium for elaeagnus mollis tissues and preparation method of anti-browning tissue culture medium
  • Anti-browning culture medium for elaeagnus mollis tissues and preparation method of anti-browning tissue culture medium
  • Anti-browning culture medium for elaeagnus mollis tissues and preparation method of anti-browning tissue culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The establishment process of the test-tube seedling of Samara oil tree of the present invention: (1) material collection; (2) disinfection, inoculation and cultivation; (3) enlarged propagation; (4) induction of roots.

[0042] According to the establishment process of the above-mentioned test-tube plantlets, the medium suitable for each stage was screened. The culture medium formula of the present invention is respectively prepared from three kinds of culture medium: primary culture medium, shoot induction medium and root induction medium, and is used as a set.

[0043] (1) Primary medium

[0044] For the primary culture of Samara oil tree, the new shoots germinated in spring or the new shoots germinated from the roots are generally used as the source of explants, and the appropriate medium is selected according to the browning rate of the explants in different mediums. In the experiment, MS, 1 / 2MS, 1 / 4MS, modified WPM, and Read were used as basic medium, and stem seg...

Embodiment 2-10

[0064] The first-generation culture medium cultivates the explant tissue of Samara oil tree, wherein, the components and the content of each component and browning rate and survival rate of every liter (L) of the first-generation culture medium are shown in the following table:

[0065] Table 1 The design scheme and browning rate and survival rate of the primary culture medium for culturing the explant tissue of Samara oleracea

[0066]

[0067] According to the formula in Example 2-10, weigh the agar powder and add it to a pot with 0.75 liters of pure water and boil it; get 0.1 liter of 10 times 1 / 2 MS mother liquor, zeatin and α-naphthaleneacetic acid and add it to the pot; weigh sucrose Add to the pot and let it dissolve; dilute to 1.0 liters with pure water; use 1mol / L hydrochloric acid or 1mol / L sodium hydroxide to adjust the pH value to 5.7; divide into 20 bottles, and then extinguish at high temperature 120°C and high pressure 0.12MPa Sterilize for 20 minutes; add Vc...

Embodiment 11-19

[0069] Bud induction medium cultivates the aseptic seedling tissue of Samara oil tree, wherein, the component of every liter (L) bud induction medium and the content of each component and browning rate and germination rate are shown in the following table:

[0070] Table 2 The design scheme and browning rate and germination rate of bud induction medium for cultivating the aseptic seedling tissue of Samara oleracea

[0071]

[0072]According to the formula in Example 11-19, weigh the agar powder and add it to a pot with 0.7 liters of pure water and boil it; take 0.1 liter of 10 times 1 / 2 MS mother liquor, dissolve it with zeatin, indole butyric acid, and banana juice and add it to the pot medium; weigh sucrose and add it to the pot and let it dissolve; dilute the volume to 1.0 liters with pure water; use 1mol / L hydrochloric acid or 1mol / L sodium hydroxide to adjust the pH to 5.7; divide into 20 bottles, and heat at 120°C , high-pressure 0.1MPa sterilization for 20 minutes; a...

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Abstract

The invention discloses an anti-browning culture medium for elaeagnus mollis tissues and a preparation method of the anti-browning tissue culture medium. The anti-browning culture medium for the elaeagnus mollis tissues comprises a primary culture medium, a bud induction culture medium and a root induction culture medium. According to the invention, through optimizing various components of the culture medium, cytokinin and auxin in the culture medium are adjusted, especially the content of Vc in the culture medium is added and adjusted, the browning rate of callus, aseptic seedlings and elaeagnus mollis seedlings is greatly reduced, the culture medium is favorable for survival, germination and rooting of the elaeagnus mollis tissues, the rooting rate of rooted seedlings is high, the rooting speed is also high, a root system is developed, the lignification of young seedlings is good, and the transplanting survival rate is high. The anti-browning culture medium for the elaeagnus mollis tissues has a simple formula, and is convenient to use and good in effect.

Description

technical field [0001] The invention relates to the technical field of tissue culture and rapid multiplication and detoxification of forest plants, in particular to an anti-browning culture medium for samara oil tree tissue and a preparation method thereof. Background technique [0002] Plant test-tube seedling technology is sterile tissue culture technology. The principle of tissue culture medium and cell pluripotency can dedifferentiate the terminally differentiated or differentiated plant tissue, induce the formation of callus, and then form new clusters on the callus bud. That is, under artificially created sterile conditions, live isolated organs (such as roots, stems, leaves, stem segments, protoplasts), tissues or cells are placed in the culture medium and placed in a suitable environment for continuous culture Techniques for obtaining cells, tissues or individuals. It is widely used in many aspects such as improving crops, propagating plants, and synthesizing compo...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCY02P60/40
Inventor 王小帆史玮张吉傲笛
Owner 西安同人农林实业股份有限公司
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