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Culture method for ramulus uncariae cum uncis

A cultivation method and technology of Uncaria, applied in the field of cultivation of Uncaria, can solve the problems of unable to meet the increasing demand, low yield and medicinal components of Uncaria, and difficulty in maintaining the humidity of Uncaria, and achieve operability and weight. Effects of good virility, reduced prevalence of root rot, and high yield of Uncaria

Pending Publication Date: 2018-04-06
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the rotary spraying method has a small coverage and is not suitable for watering Uncaria shrubs; drip tanks need to lay complex and multi-channel pipes on the ground, occupying the growth area of ​​Uncaria, and it is difficult to maintain the humidity required for the growth of Uncaria. ;Using the traditional watering method from the ground with water pipes, the water consumption is large, the watering amount is uneven, and the time is long. It is easy to cause some uncariats to rot due to excessive water absorption. The medicine for root rot, the obtained Uncaria is not a pure natural, green and environmentally friendly Uncaria, which increases the risk of using Uncaria as a medicine
In fact, the yield and medicinal components of Uncaria uncaria obtained through the cultivation of ramifications, cuttings, seeds, etc. combined with traditional watering methods are low, which can no longer meet the increasing demand

Method used

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  • Culture method for ramulus uncariae cum uncis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Step 1. Select mature and plump Uncaria seeds, soak and sterilize them in sequence, and then transfer them to the seed medium for cultivation for 20-25 days to obtain hypocotyls of sterile seedlings. The seed medium is a hormone-free MS medium;

[0035] Step 2, cutting the hypocotyl explants without cotyledon nodes with a length of 0.4 cm from the hypocotyls of sterile seedlings, placing the hypocotyl explants flat in the first callus culture medium for dark culture, after 20 days , the two ends of the hypocotyl explant expand to form the hypocotyl callus, and then the hypocotyl callus is transferred to the second callus medium for dark culture for 20 days to obtain fresh callus, the first The callus medium comprises MS, 1.0mg / L of TDZ, the NAA of 0.03mg / L, and the second callus medium comprises MS, 1.0mg / L of TDZ, 0.03mg / L of NAA, 80mg / L L colchicine;

[0036]Step 3, the fresh callus is transferred to the differentiation medium for differentiation culture for 20 days ...

Embodiment 2

[0041] Step 1, select mature and plump Uncaria seeds, soak and sterilize them in turn, and transfer them to the first medium for cultivation for 20-25 days to obtain hypocotyls of sterile seedlings. The seed medium is a hormone-free MS medium;

[0042] Step 2, cutting out hypocotyl explants with a length of 0.6 cm from the hypocotyls of sterile seedlings without cotyledons, placing the hypocotyl explants flat in the first callus medium for dark culture, and after 23 days , the two ends of the hypocotyl explants expand to form the hypocotyl callus, and then the hypocotyl callus is transferred to the second callus medium for dark culture for 15 days to obtain fresh callus, the first The callus medium comprises MS, 1.2mg / L of TDZ, the NAA of 0.04mg / L, and the second callus medium comprises MS, 1.2mg / L of TDZ, 0.04mg / L of NAA, 150mg / L L colchicine;

[0043] Step 3, transfer the fresh callus to the differentiation medium and carry out differentiation culture for 23 days to obtain ...

Embodiment 3

[0048] Step 1, select mature and plump Uncaria seeds, soak and sterilize them in turn, and transfer them to the first medium for cultivation for 20-25 days to obtain hypocotyls of sterile seedlings. The seed medium is a hormone-free MS medium;

[0049] Step 2, cutting the hypocotyl explants without cotyledonous nodes with a length of 0.8 cm from the hypocotyls of sterile seedlings, placing the hypocotyl explants flat in the first callus medium for dark culture, and after 25 days , the two ends of the hypocotyl explants expand to form the hypocotyl callus, and then the hypocotyl callus is transferred to the second callus medium for dark culture for 7 days to obtain fresh callus, the first The callus medium comprises MS, 1.5mg / L of TDZ, the NAA of 0.05mg / L, and the second callus medium comprises MS, 1.5mg / L of TDZ, 0.05mg / L of NAA, 200mg / L L colchicine;

[0050] Step 3, the fresh callus is transferred to the differentiation medium to carry out differentiation culture for 25 day...

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Abstract

The invention discloses a culture method for ramulus uncariae cum uncis. The culture method comprises the following steps: firstly, culturing a ramulus uncariae cum uncis seed in a seed culture mediumfor 20-25d to obtain a sterile seedling hypocotyl; secondly, cutting a hypocotyl explant having the length of 0.4-0.8cm and containing no cotyledonary nodes from the sterile seedling hypocotyl, horizontally placing the hypocotyl explant in a first callus culture medium for dark culture for 20-25d, and then, transferring the hypocotyl explant into a second callus culture medium for dark culture toobtain a fresh callus; thirdly, transferring the fresh callus into a differential culture medium for differential culture to obtain a cluster bud; and fourthly, transplanting the cluster bud into a strong seedling culture medium for strong seedling culture, cutting off a basal part of a stem when the length of a single seedling is 3-4cm, transferring the seedling into a rooting culture medium forrooting culture, and growing a root with the length of 1.5-2.0cm to obtain a ramulus uncariae cum uncis seedling. The ramulus uncariae cum uncis obtained by using the culture method is purely natural, high in yield and high in medicinal component content.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for cultivating Uncaria. Background technique [0002] Uncaria is one of the commonly used Chinese medicinal materials. It was first recorded in "Bielu of Famous Doctors" written by Tao Hongjing in the Liang Dynasty. It has a long history of medicinal use. Uncaria is sweet and cool in nature and has the effects of clearing away heat, calming the liver, relieving wind and relieving convulsions, and its main chemical components are rhynchophylline and isorhynchophylline. It is widely used clinically for headache, high blood pressure, convulsions, neurasthenia and other diseases. Uncaria is the main raw material of Uncaria Tablets, Zhengtian Pills, Tianma Uncaria Granules, Maijunan and many other famous Chinese medicine varieties and commonly used medicines in traditional Chinese medicine formulas. Bad work and rest habits have led to an increasing number of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G22/00
CPCA01H4/001A01H4/008
Inventor 黄浩白隆华韦莹翟勇进韦树根
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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