31 results about "Antiporters" patented technology

The present invention relates to the cloning, recombination and salt tolerance analysis of cotton Na+ / H+ reverse transport protein GhNHX1 gene, and belongs to the field of molecular biology and biological technology. Total RNA is extracted from cotton leaf treated with 0.4 mol / L NaCl solution and inversely transcribed into cDNA. Facultative primer is used for conventional PCR to obtain intermediate segment, and whole length cDNA is obtained through fast amplification in 3' and 5' ends. The gene is transformed to salt-sensitive yeast mutant to restore its salt tolerant capacity to some degree; and the gene is further transformed to tobacco to obtain transgenic plant capabl eof growing normally in salt concentration of 200 mmol / L. Therefore, the gene is one important salt tolerant gene and may be used in raising the salt tolerant capacity of plant for plantation in saline land.

The invention relates to the field of plant gene engineering, providing a buckwheat Na+ / H+ antiporter FtNHX. The buckwheat Na+ / H+ antiporter FtNHX is provided with SEQ ID No:2 amino acid residue sequence in a sequence table, or the buckwheat Na+ / H+ antiporter FtNHX is obtained by the substitution, deletion or adding of one or multiple amino acid residues by SEQ ID No: 2 in the sequence table, has the same activity with the SEQ ID No:2 amino acid residue sequence and is derived by SEQ ID No:2. The gene for coding the protein is preferably selected from a nucleotide sequence comprising the 288th-1949th bit of SEQ ID No:1, or the gene is a nucleotide sequence which has more than 90% of homology with a DNA sequence specified by the SEQ ID No:1 and codes same functional protein. The invention also provides methods for recombinant vector construction, transgenic plant and the like to apply the above gene and protein, and can cultivate new improved gene plant species with stronger salt tolerance or other biological characters.

A method for cultivating sugar beet with strong salt tolerance and drought resistance, the purpose of which is to avoid the defects of traditional breeding and the lack of previous techniques for single gene transformation, and realize the combination of two Na+ / H+ antiporter genes ZxNHX and vacuolar Membrane H+-pyrophosphatase gene ZxVP1-1 is polymerized, and a new transgenic sugar beet variety (line) co-expressing ZxNHX and ZxVP1-1 is obtained through genetic transformation, providing a method for cultivating sugar beets with strong salt tolerance and drought resistance and high sugar content; The steps include seedling cultivation, seedling infection, molecular identification and stress resistance evaluation. This method greatly shortens the cultivation time of transgenic seedlings, the whole cycle is controlled at about 5 months, no callus is required, and the genetic transformation efficiency is as high as 10%.

The present invention belongs to the cloning, recombination and salt endurance function analysis of couchgrass sodium ion reverse transport protein ENHX1 gene, and belongs to the field of molecular biology and biological technology. General RNA is first extracted from couchgrass root and then inverse transcripted into cDNA. A pair of facultative primer is designed based on the conservative amino acid sequence of other plant's sodium ion reverse transport protein CycD2 for conventional PCR, land the PCR product is connected to pGEM-The vector to convert DH5 alpha cell and determine sequence. Then, whole length cDNA is obtained via fast amplification in 3' and 5' ends. Further, positive-sense expression vector is constituted to convert pseudomustard.

The present invention relates to cloning, recombination, salt endurance function analysis and applications of Na+ / H+ reverse transport protein MsNHX genes in alfalfa, and belongs to molecular biology and biological technology field. The total RNA is extracted from roots of alfalfa, then 1 microgramme total RNA is reversely transcripted into cDNA. According to conservative amino acid sequences in Na+ / H+ reverse transport protein in other plants, a pair of degenerated primers are designed to execute a routine polyase chain reaction (PCR), wherein the PCR product is connected to a pGEM-T carrier to converse DH5 alpha cells and execute sequence determination. Then, 3' and 5' tail ends rapid amplifications are executed to obtain cDNA with full-length. Right expression carriers are further constructed for conversion of arabidopsis thaliana. The transgenic arabidopsis thaliana possesses higher salt resistant capability. Hence the transgenic double-seminal leaf crops such as heart trefoil will improve its salt resistant capability, yield and quality, then the planted area of dicotyledon at alkaline land can be expanded to generate huge economic benefit and social benefit.