Cadaverine-producing engineering strain

Abstract

The invention relates to a cadaverine-producing engineering strain, a construction method thereof and a method for producing cadaverine by using the strain. Based on a gene engineering technology, an L-lysine decarboxylase gene (cadA / LDC) and a cadaverine-lysine antiporter gene (cadB) are respectively or simultaneously cloned into Corynebacterium glutamicum and subjected to induced coexpression, the lysine is decarboxylated to generate cadaverine under the catalytic action of the CadA enzyme, and the cadaverine is transported out of cells by the CadB protein, thus constructing a high-yield cadaverine-producing engineering strain and establishing a novel one-step cadaverine synthesis method based on the fermentation of Corynebacterium glutamicum. The one-step cadaverine synthesis method based on microbial fermentation provides a new way for large-scale cadaverine production, thereby having great economic benefits and social benefits and wide market development prospects.

Description

technical field [0001] The invention belongs to the field of genetic engineering bacteria, in particular to a cadaverine-producing engineering bacteria. Background technique [0002] Cadaverine (Cadaverine) is a polyamine, that is, 1,5-pentanediamine (pentamethylenediamine for short), which is produced by decarboxylation of lysine in organisms, and is a biologically active nitrogen-containing compound widely present in organisms. Alkaline, but also present in spoilage as a carnitine. Cadaverine is an important raw material for the synthesis of new materials polyamide-54 (condensed from cadaverine and succinic acid) and polyamide-56 (condensed from cadaverine and oxalic acid), and has important industrial applications. [0003] Currently, there are two methods for synthesizing cadaverine: chemical synthesis and enzymatic conversion. The chemical synthesis method has harsh conditions and pollutes the environment, while the enzymatic conversion method has a complicated proces...

AI Technical Summary

Problems solved by technology

Although Escherichia coli, Bacillus cadaverium, Hafnia hives, etc. can directly synthesize cadaverine, and the regulation of cadaverine synthesis has been extensively studied, these bacteria cannot synthesize lysine in large quantities, and the synthesis of cadaverine is low, so they are not suitable for direct fermentation. Production

Corynebacterium glutamicum can synthesize a large amount of L-lysine, but cannot synthesize lysine decarboxylase, nor can it transport cadaverine, which brings certain difficulties to the fermentation of cadaverine

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