The present invention discloses a method for researching the role of melatonin in predicting the outcome of ovarian reserve and in vitro fertilization-embryo transfer (IVF-ET), including: collecting basic clinical data of 61 women, and according to their ovary response to stimulation, mainly by age , the number of retrieved eggs, and the value of anti-Müllerian hormone were divided into three groups: ovarian low response group, normal group and high response group. The follicular fluid of 61 women was collected, the melatonin concentration in the follicular fluid of 61 women was measured by melatonin radioimmunoassay, and the melatonin concentration and other clinical data were analyzed in the ovarian low response group, normal group and high response group. level; analyze the relevant data of in vitro fertilization outcome in ovarian low response group, normal group, and high response group, and verify the correlation between melatonin concentration and in vitro fertilization-embryo transfer outcome; verify the relationship between melatonin level and ovarian Relevance of reserves. The invention can predict the outcome of ovarian reserve and in vitro fertilization-embryo transfer by detecting the melatonin concentration in the follicular fluid.