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Molecular genetic marker associated with egg-laying traits of ducks and application of molecular genetic marker

A technology of molecular genetic markers and traits, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems that there is no molecular marker of AMH gene, etc., and achieve the effect of rapid detection method

Active Publication Date: 2017-05-31
INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, there are few reports on the AMH gene in laying ducks. So far, there is no research on the AMH gene in laying ducks as a molecular marker for duck laying traits.

Method used

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  • Molecular genetic marker associated with egg-laying traits of ducks and application of molecular genetic marker
  • Molecular genetic marker associated with egg-laying traits of ducks and application of molecular genetic marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Collection of duck blood samples and extraction of DNA:

[0022] After the pedigree data and egg production performance of the tested ducks are measured, about 1ml of blood is drawn from the duck wing vein with a disposable syringe, and injected into a 1.5ml centrifuge tube that has been sterilized by high pressure and filled with 200μL of sterile ACD anticoagulant Shake gently, record the wing number, and store at -20°C for later use.

[0023] Genomic DNA was extracted using a DNA extraction kit and operated according to the kit instructions. The specific steps were to take 10 μL duck blood and add it to a 1.5ml centrifuge tube, then add 20 μL proteinase K and 0.5ml binging buffer to the centrifuge tube, and vortex After shaking for 15 seconds, let stand at room temperature for 10 minutes. The resulting solution was added to an adsorption column, centrifuged at 12,000 rpm for 1 minute, and the waste liquid was discarded. Put the adsorption column back into the col...

Embodiment 2（ application Embodiment ）

[0029] In a group of 320 Jingjiang ducks, the detection application of association analysis was carried out on the different genotypes of duck AMH gene and duck laying traits (including the age at first laying, the number of eggs laid at 43 weeks, and the number of eggs laid at 72 weeks). The results of genotype detection showed that among the 320 individuals detected, GG genotype individuals accounted for the majority, with 182 individuals, GA genotype individuals had 90 individuals, and AA genotype individuals had the least number of individuals, with 48 individuals. Different genotypes and traits The results of the association analysis are shown in Table 1.

[0030] It can be concluded from Table 1 that this mutation site is significantly correlated with the number of eggs laid at 43 weeks and 72 weeks of laying ducks (P<0.05). The above analysis shows that there is a significant or significant relationship between the gene mutation at this site and the above traits. very s...

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Abstract

The invention relates to a molecular genetic marker associated with egg-laying traits of ducks and application of the molecular genetic marker, belongs to the field of poultry breeding, and particularly relates to application of a G575A site of an AMH (anti-Mullerian hormone) gene, associated with egg-laying traits of ducks, serving as a molecular genetic marker in duck production performance research. A specific DNA segment is obtained from the AMH gene to serve as a molecular marker associated with the egg-laying traits of the ducks, one G575-A575 basic group in a sequence of the molecular marker mutates, the mutation site is significantly associated with the 43-week-old egg production and the 72-week-old egg production of the ducks (P smaller than 0.05), and by performing PCR (polymerase chain reaction) amplification and performing sequencing-based typing on a PCR product, a GG genotype is selected as an laying duck. A detection method provided by the invention is significant in effect, is simple and quick, and is not influenced by an external environment.

Description

technical field [0001] The invention belongs to the field of poultry breeding, and in particular relates to a method for increasing the number of eggs laid by duck flocks by using homozygous compound genotypes. Background technique [0002] For a long time, laying duck breeding in my country has been dominated by group breeding, and family selection is often used in breeding. The low-yielding group in the laying duck group is an important factor affecting the egg production rate of the duck group and affecting the breeding efficiency, which can cause huge economic losses. The number of eggs laid by laying ducks is affected by multiple factors such as genetics, environment, nutrition, and feeding management. At present, laying ducks are mostly produced by strengthening feeding management, supplemented by comprehensive measures of nutritional regulation and environmental improvement. Through the above method to a certain extent, the egg production level of the laying duck pop...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 张昊陈芳梁振华杜金平吴艳皮劲松潘爱銮申杰蒲跃进
Owner INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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