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Core promoter for influencing cow mastitis infectibility/resistance HMGB3 gene and functional molecule mark and application

A mastitis and promoter technology, applied in the field of biochemical genetics and breeding, can solve the problems that the functional research of expression regulation mode has not been reported yet, and achieve the effects of reducing economic losses, improving the ability and reducing the incidence

Inactive Publication Date: 2015-02-18
DAIRY CATTLE RES CENT SHANDONG ACADEMY OF AGRI SCI
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AI Technical Summary

Problems solved by technology

So far, the expression regulation pattern of the two transcripts of the bovine HMGB3 gene and its functional study in cow mastitis have not been reported

Method used

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  • Core promoter for influencing cow mastitis infectibility/resistance HMGB3 gene and functional molecule mark and application
  • Core promoter for influencing cow mastitis infectibility/resistance HMGB3 gene and functional molecule mark and application
  • Core promoter for influencing cow mastitis infectibility/resistance HMGB3 gene and functional molecule mark and application

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Experimental program
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Effect test

Embodiment 1

[0067] 265 Chinese Holstein cows were used as experimental animals, and the blood samples of these cows came from dairy farms in Qingdao and Jinan. The DHI data for these 265 cattle were fully documented.

[0068] HMGB3F / R primers were used to amplify the DNA fragments of cattle, and the g.+2690 site of the HMGB3 gene was genotyped in the above 265 dairy cows by direct DNA sequencing. At the same time, statistical analysis was performed on the correlation between the genotype of this SNP site and the SCS phenotype in the DHI record, as shown in Table 4.

[0069] Table 4 Correlation analysis between bovine HMGB3 gene SNP (g.+2690C>T) genotype and somatic cell score

[0070]

[0071] The analysis results showed that the SNP site g.+2690C>T of the HMGB3 gene, the T allele was the resistance gene, and the C allele was the susceptibility gene. The somatic cell score of genotype TT individuals was the lowest, indicating that TT genotype individuals had higher resistance to mast...

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Abstract

The invention provides a core promoter for influencing cow mastitis infectibility / resistance HMGB3 gene and a functional molecule mark and an application, which belongs to the biochemistry genetic breeding technical field. The concrete method is characterized in that a cow mastitis infectibility / resistance HMGB3 candidate gene is provided through inverse transcription RT-PCR and real time fluorescence quantification RT-qPCR, a functional SNP site information of a cow HMGB3 gene P3 promoter zone is used, a molecular biology correlation technology is used for discriminating the genotype of the cow at this site, and the genotype having mastitis resistance is selected. The favorable genotype is selected for keeping species, the frequency of the HMGB3 gene resistance genotype in a cow colony can be enhanced, thereby the capability of anti-mastitis by the cow colony is enhanced, the incidence of disease of the cow mastitis is reduced, and the economy loss is reduced. The invention provides the novel method for mastitis resistance genetic improvement.

Description

technical field [0001] The invention relates to the technical field of biochemical genetic breeding, in particular to a functional molecular marker site in the promoter region of the HMGB3 gene that affects susceptibility / resistance to mastitis in cows and its application in cow genetic improvement. Background technique [0002] Mastitis is one of the most common and complex diseases in dairy cattle feeding, and it is also the disease that causes the greatest loss in the dairy industry. It is an inflammatory change in mammary gland tissue that is stimulated by physics, chemistry, and microorganisms. It is characterized by an increase in somatic cells in milk, pathological changes in mammary gland tissue, and abnormal milk properties and quality. It is estimated that about 95% of cases are caused by Staph.aureus, Strep.uberis and E.coli infections, and the annual loss due to mastitis is as high as 35 billion US dollars ( Wellenberg et al., 2002). The economic losses caused ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/113C12N15/11
CPCY02A40/70
Inventor 黄金明王秀革李黎明鞠志花齐超张燕李秋玲王长法李荣岭杭苏琴侯明海高运东仲跻峰
Owner DAIRY CATTLE RES CENT SHANDONG ACADEMY OF AGRI SCI
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