Application of SNP molecular marker in identification of high-reproductive-performance dairy cows and assisted breeding
A technology of molecular markers and reproductive performance, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of less research on genetic mechanism and the urgent need to strengthen the development of molecular markers, so as to improve the reproductive efficiency , reduce feeding costs and improve breeding efficiency
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Embodiment 1
[0042] The 7th exon of the SIGLEC12 gene affecting the gestation cycle of dairy cows and its molecular markers and applications of the present invention will be described in detail below.
[0043] The present invention affects the 7th exon of the SIGLEC12 gene of the dairy cow gestation cycle and its molecular marker and application, and its specific scheme is:
[0044] 1. Screening SIGLEC12 gene as a candidate gene for dairy cow gestation cycle
[0045] (1) Blood or hair follicle samples were collected from 2,709 Holstein cows in 11 large-scale farms
[0046] Holstein cattle samples were collected in 11 pastures across the country, and the location of the pastures and the number of samples collected in each pasture are shown in Table 1.
[0047] Table 1. Location of pastures where samples were collected and number of samples collected
[0048] The location of the ranch Number of samples The location of the ranch Number of samples Jinchang, Gansu 294 ...
Embodiment 2
[0067] The Sanger sequencing method was used to detect the genotype and allele frequency distribution of the 3 SNP sites of the 7th exon of SIGLEC12 in the Holstein cattle population. The results are shown in Table 2, including the Holstein cattle. The test results showed that there were two genotypes in the Holstein cow population in my country; among all the detected populations, the frequency of GAT / GAT haplotype combination was 97.8%, the frequency of TGC / GAT haplotype combination was 2.2%, and the frequency of GAT haplotype Type is dominant haplotype (see Table 2).
[0068] Table 2 Distribution results of bovine SIGLEC12 gene in Holstein cattle population
[0069]
[0070] Table 2 shows that the above-mentioned group materials are Holstein cattle groups in my country, among which: group 1 is a Holstein cattle group in a ranch in Gansu, group 2 is a Holstein cattle group in a ranch in Hebei, and group 3 is a Holstein cattle group in a ranch in Inner Mongolia Cattle herd...
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