A molecular marker of nfat5 gene related to growth traits of goat and its application
A technology of growth traits and molecular markers, which is applied in recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems that are not available, affect the number of litters, etc., and achieve the effect of rapid detection method
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Embodiment 1
[0023] Example 1 Acquisition of goat NFAT5 gene SNP detection fragment and establishment of detection method
[0024] 1. Extraction of goat genomic DNA
[0025] The test goat breeds of the present invention are Macheng black goat, Boer goat and black-headed sheep (a hybrid of Boer goat and Macheng black goat), and the samples are all from the sheep farm of the Animal Husbandry and Veterinary Research Institute of Hubei Academy of Agricultural Sciences. Goat genomic DNA was extracted using the blood genomic DNA extraction kit (Item No.: DP348) produced by Beijing Tiangen Biochemical Technology Co., Ltd. The specific steps were referred to the kit instructions. The extracted DNA was tested for concentration and quality, and stored at -20°C for later use.
[0026] 2. Acquisition of goat NFAT5 gene SNP genetic marker detection fragment
[0027] (1) PCR amplification
[0028] A pair of primers were designed according to the SNP genetic marker detection sequence (shown in SEQ ID ...
Embodiment 2
[0038] Example 2 Detection of polymorphism distribution of molecular markers prepared by the present invention in goat population
[0039] In this example, the polymorphism of the c.-454C>G site in the 5' flanking sequence of the goat NFAT5 gene was detected in the populations of Boer goat, black-headed sheep and Macheng black goat respectively, and the detection results are shown in Table 1.
[0040] Table 1 - Genotype frequency and allele frequency of goat c.-454C>G locus in different goat populations
[0041]
[0042] From the results in Table 1, it can be seen that the c.-454C>G locus has three genotypes in Boer goats and black-headed sheep, except for the absence of GG genotype in Macheng black goats, among which the genotype is homozygous Predominantly, the GG genotype was present in a lower proportion. The dominant alleles of the Boer goat, black-headed sheep and Macheng black goat populations were consistent, and the c.-454C>G locus was the dominant allele in the t...
Embodiment 3
[0043] Example 3 Association analysis and application of goat NFAT5 c.-454C>G molecular markers prepared by the present invention and one-year-old growth traits
[0044] In order to determine whether the c.-454C>G site in the 5' flanking sequence of the goat NFAT5 gene is related to the difference in the growth traits of black-headed sheep, the method established in Example 1 was used for polymorphism detection, and the c.-454C in the goat NFAT5 gene was analyzed. >Correlation of three genotypes of G locus with one-year-old growth traits in goats. SAS statistical software (SAS Institute Inc, Version 9.1) GLM program was used to analyze the association between c.-454C>G locus genotype and one-year-old growth traits, and a significant test was performed. The model used was:
[0045] Y ikjlm =μ+G i +F k +A j +S l +P m +e ikjlm
[0046] Y is the phenotypic value of the trait, μ is the average value of a single trait, G i is the genotype effect, F k is the field fixed eff...
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