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NFAT5 gene molecular marker related to goat growth traits and application of NFAT5 gene molecular marker

A technology of growth traits and molecular markers, which is applied in recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems that are not available, affect the number of litters, etc., and achieve the effect of rapid detection method

Active Publication Date: 2021-01-05
INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have found that NFAT5 can significantly promote the proliferation of mouse follicular granulosa cells, indicating that it may regulate the development of follicles, thereby affecting the number of litters
So far, there is no relevant report on the study of goat NFAT5 gene as a molecular marker for goat growth traits

Method used

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  • NFAT5 gene molecular marker related to goat growth traits and application of NFAT5 gene molecular marker
  • NFAT5 gene molecular marker related to goat growth traits and application of NFAT5 gene molecular marker
  • NFAT5 gene molecular marker related to goat growth traits and application of NFAT5 gene molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Obtaining of Goat NFAT5 Gene SNP Detection Fragment and Establishment of Detection Method:

[0024] 1. Extraction of Goat Genomic DNA

[0025] The test goat breeds of the present invention are Macheng black goat, Boer goat and black-headed goat (a hybrid of Boer goat and Macheng black goat). The goat genomic DNA was extracted using the Blood Genomic DNA Extraction Kit (Product No.: DP348) produced by Beijing Tiangen Biochemical Technology Co., Ltd., and the specific steps were referred to the kit instructions. The concentration and quality of the extracted DNA were tested and stored at -20°C for future use.

[0026] 2. Obtaining the detection fragment of goat NFAT5 gene SNP genetic marker

[0027] (1) PCR amplification

[0028] According to the SNP genetic marker detection sequence (as shown in SEQ ID NO.1) in the genome sequence of goat NFAT5 gene (Gene ID: 102173116), a pair of primers are designed to amplify the fragment of the polymorphic site ( figure...

Embodiment 2

[0038] Example 2 Detection of polymorphism distribution of molecular markers prepared in the present invention in goat populations:

[0039] In this example, the polymorphism of the c.-454C>G site in the 5' flanking sequence of the goat NFAT5 gene was detected in the populations of Boer goat, black-headed goat and Macheng black goat, and the detection results are shown in Table 1.

[0040] Table 1-Genotype frequency and allele frequency of goat c.-454C>G loci in different goat populations

[0041]

[0042] From the results in Table 1, it can be seen that the c.-454C>G locus exhibits three genotypes in Boer goats and black-headed sheep populations, except that there is no GG genotype in Macheng black goats, and the genotypes are homozygous Occupying an advantage, the GG genotype has a low proportion. The dominant alleles in the Boer goat, Black-head sheep and Macheng black goat populations were consistent, and the c.-454C>G site allele C was the dominant allele in the three...

Embodiment 3

[0043] Example 3 Correlation Analysis and Application of Goat NFAT5 c.-454C>G Molecular Marker Prepared in the Present Invention and Yearling Growth Traits:

[0044] In order to determine whether the c.-454C>G site in the 5' flanking sequence of the goat NFAT5 gene is related to the difference in the growth traits of black-headed sheep, the method established in Example 1 was used for polymorphism detection, and the c.-454C in the goat NFAT5 gene was analyzed >Correlation between three genotypes of G loci and yearling growth traits of goats. The SAS statistical software (SAS Institute Inc, Version 9.1) GLM program was used to carry out the correlation analysis between the c.-454C>G locus genotype and the growth traits at one year old, and the significance test was carried out. The model used was:

[0045] Y ikjlm =μ+G i +F k +A j +S l +P m +e ikjlm

[0046] Y is the phenotype value of the trait, μ is the average value of a single trait, G i is the genotype effect, F ...

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Abstract

The invention provides an NFAT5 gene molecular marker related to goat growth traits and application of the NFAT5 gene molecular marker. The molecular marker is located in a 5' flanking sequence of a goat NFAT5 gene; a nucleotide sequence of the molecular marker is shown as a sequence table SEQ ID NO.1, the length of the sequence is 392bp, the sequence comprises a part of the 5' flanking sequence (-1bp to -238bp) and a part of a 5' untranslated region sequence (+1bp to +154bp), a C>G base mutation exists at the 222bp in the sequence, and the mutation is named as c.-454C>G. The invention also provides an SNP detection kit associated with the goat growth traits, and the SNP detection kit comprises a primer pair shown as SEQ ID NO.2 to 3 and an SNaPshot single base extension primer shown as SEQ ID NO.4. A new molecular marker associated with the goat growth traits is explored, early selection of the goat growth traits is realized, and a detection method is fast and accurate and is not affected by breeding environment condition factors.

Description

technical field [0001] The invention relates to the technical field of goat molecular marker screening, in particular to an NFAT5 gene molecular marker related to goat growth traits and an application thereof. Background technique [0002] my country has a long history of raising goats and is a common source of meat for residents. In recent years, the scale of goat farming in the world has continued to expand, especially in China, which is a big goat farming country. With the continuous growth of consumers' requirements for healthy diet, the proportion of mutton in meat consumption is constantly increasing due to its healthy and safe nutritional characteristics. Growth traits including body weight and body size are the most important economic traits in goat production. Therefore, it is of great significance to study how to improve the growth traits of goats through breeding for goat industry and goat breeding research. [0003] Single nucleotide polymorphism (Single nucle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2531/113C12Q2533/101
Inventor 陶虎陈明新刘洋张年熊琪杨娟李晓锋索效军杨前平田宏张鹤山熊军波张凤
Owner INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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