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Preparation and application of anti-Mullerian hormone (AMH) monoclonal antibody

A monoclonal antibody, AMH-2 technology, applied in the biological field, can solve problems such as ovarian hyperstimulation syndrome

Inactive Publication Date: 2019-11-05
江苏正大天创生物工程有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

If the AMH value is greater than 6.8, you may consider the constitution of polycystic ovary syndrome. When using ovulation injections and drugs, the ovaries are prone to overreact and excrete too many eggs, resulting in ovarian hyperstimulation syndrome.

Method used

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  • Preparation and application of anti-Mullerian hormone (AMH) monoclonal antibody
  • Preparation and application of anti-Mullerian hormone (AMH) monoclonal antibody
  • Preparation and application of anti-Mullerian hormone (AMH) monoclonal antibody

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Embodiment Construction

[0011] The present invention will be further described below in conjunction with the embodiments and accompanying drawings.

[0012] 1. Obtaining of hybridoma cells and preparation of monoclonal antibodies

[0013] 1. Animal Immunization

[0014] The immunogen is a recombinant expression protein (59KD) of Escherichia coli, the coding sequence is NP_000470 (Arg26-Arg560), purchased from Beijing Aibison Biotechnology Co., Ltd., the product number is TP710250. BALB / c female mice weighing 18-20 g at the age of 6 weeks were immunized with this recombinant protein. That is, AMH recombinant protein 100 μg / mouse was mixed with Freund’s complete adjuvant, fully emulsified, and 0.3 mL / mouse was injected subcutaneously through the back and abdomen at multiple points, and then 80 μg / mouse of antigen and Freund’s incomplete adjuvant were taken every 2 weeks. After the agent was fully emulsified, 0.3 mL / mouse was injected intraperitoneally, and one week after the fourth immunization, bloo...

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Abstract

The invention relates to a human anti-Mullerian hormone (AMH) antibody and application thereof. Various antibodies are prepared and subjected to pairing screening, and an antibody combination is obtained, wherein the sensitivity and specificity of the antibody combination both can meet demands.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to two hybridoma cell lines secreting pairable anti-AMH monoclonal antibodies and the application of the monoclonal antibodies. Background technique [0002] Anti-Mullerian hormone (AMH) is a member of the transforming growth factor β superfamily, which was first discovered by Professor Alfred Jost in 1974. AMH is a dimeric glycoprotein composed of two identical 70KD subunits connected by disulfide bonds, with a relative molecular mass of 140KD; the human AMH encoding gene is located on the short arm of chromosome 19, with a size of 2.4-2.8kb and contains 5 exosomes. Exon. [0003] Anti-Müllerian hormone (AMH) plays an important role in the development of gonad organs and is one of the important markers of gonad function in men and women. In males, AMH is mainly produced by Leydig cells of the testis, starting from embryogenesis and throughout life; during the development of male fetu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/26G01N33/74G01N33/577
CPCC07K16/26G01N33/577G01N33/74
Inventor 李思慧魏赵延林伟强吴凯朱庆平
Owner 江苏正大天创生物工程有限公司
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