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Anti-mullerian hormone (AMH) antibody and preparation method thereof

A technology of antibody and polyclonal antibody, which is applied in the field of immunity, can solve the problems of poor detection stability and low sensitivity, and achieve the effects of high detection stability, reduced production cost and high detection sensitivity

Inactive Publication Date: 2017-05-17
刘玲
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Problems solved by technology

[0005] However, when an antigen is detected using the above-mentioned anti-AMH antibody, there are problems of poor detection stability and low sensitivity

Method used

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  • Anti-mullerian hormone (AMH) antibody and preparation method thereof
  • Anti-mullerian hormone (AMH) antibody and preparation method thereof
  • Anti-mullerian hormone (AMH) antibody and preparation method thereof

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Experimental program
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Embodiment

[0039]

[0040] Immunogen preparation:

[0041] The AMH gene sequence (SEQ ID NO: 7) was synthesized by Suzhou Synbio Biotechnology Co., Ltd., and its basic information is shown below.

[0042] 1. Transform the pET-28a(+) synthesized above with the AMH gene into DH5α competent bacteria to amplify and extract the plasmid.

[0043] 2. Transform the plasmid into the expression strain Bl21(DE3), pick the bacteria to detect and preserve the species.

[0044] 3. Induced expression of protein.

[0045] 1) Shake the expression strain in 3ml LB medium to OD 600 =0.6 or so, add IPTG, the concentration gradient is from 25μM to 1mM. Induce overnight at 37 degrees (generally, there will be a large amount of expression after more than 3 hours).

[0046] 2) SDS-PAGE electrophoresis to detect the expression of the target protein. Note: The expression amount of the target protein inclusion body will generally reach more than 50% of the bacterial protein, and obvious thick bands can be ...

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Abstract

The invention provides an anti-mullerian hormone (AMH) antibody and a preparation method thereof. The AMH antibody is prepared by selecting amino acid sequences of an appropriate position and length as an immunity antigen to immunize an animal and further by optimizing the antibody. The anti-AMH antibody provided by the invention cannot be combined with unstable amino acid sequences between the 26th site and the 59th site in the full-length AMH, so that the immunity reaction intensity is improved, and the detection sensitivity is greatly improved.

Description

technical field [0001] The invention relates to the technical field of immunization, in particular to an anti-Müllerian hormone antibody and a preparation method thereof. Background technique [0002] Anti-Mullerian hormone (AMH) is a kind of monosaccharide protein secreted by immature Sertoli cells of the testis and granulosa cells of ovarian follicles. It belongs to the transforming growth factor β superfamily and has the ability to inhibit the male Mullerian Development, regulation of important functions of germ cells and gonad development of both sexes. In recent years, scholars from various countries have conducted extensive research on its role in the field of female reproduction. In women, AMH can regulate follicle development, reflect ovarian reserve function, and predict ovarian response to superovulation, which has certain value for the research of reproductive endocrine diseases. [0003] At present, there are many records about the detection of AMH by immunoass...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/22C12N5/10
CPCC07K16/22
Inventor 刘玲张明程
Owner 刘玲
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