Anti-mullerian hormone (AMH) antibody and preparation method thereof
A technology of antibody and polyclonal antibody, which is applied in the field of immunity, can solve the problems of poor detection stability and low sensitivity, and achieve the effects of high detection stability, reduced production cost and high detection sensitivity
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[0039]
[0040] Immunogen preparation:
[0041] The AMH gene sequence (SEQ ID NO: 7) was synthesized by Suzhou Synbio Biotechnology Co., Ltd., and its basic information is shown below.
[0042] 1. Transform the pET-28a(+) synthesized above with the AMH gene into DH5α competent bacteria to amplify and extract the plasmid.
[0043] 2. Transform the plasmid into the expression strain Bl21(DE3), pick the bacteria to detect and preserve the species.
[0044] 3. Induced expression of protein.
[0045] 1) Shake the expression strain in 3ml LB medium to OD 600 =0.6 or so, add IPTG, the concentration gradient is from 25μM to 1mM. Induce overnight at 37 degrees (generally, there will be a large amount of expression after more than 3 hours).
[0046] 2) SDS-PAGE electrophoresis to detect the expression of the target protein. Note: The expression amount of the target protein inclusion body will generally reach more than 50% of the bacterial protein, and obvious thick bands can be ...
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