The invention mainly relates to the technical field of planting, and discloses a seeding and seedling culture method of strawberry. The seeding and seedling culture method comprises the following steps of seed soaking, seed softening, seed germination accelerating, seeding, and postemergence management. The seeding and seedling culture method has the advantages that the germinating rate of strawberry seed is 89%, the germinating time is shortened to 5 to 6 days, the planting efficiency is improved, and the economic income is increased by 12.4%; after the strawberry seed is soaked by a dietary alkali solution, the pH (potential of hydrogen) value is increased, the seed coat is softened, the germ activity is improved, and the seed germinating is accelerated; after enzymolysis by composite enzyme, the structure of the seed coat is primarily destroyed, the water and nutrients are accelerated to enter, and the germinating is accelerated; the most suitable germinating condition is maintained during germination acceleration, a germination accelerating agent is sprayed to maintain humidity, the bacteria inhibiting and insect killing functions are realized, the nutrients are provided, and the strawberry seed is promoted to quickly germinate; after germinating, the light radiation duration is extended, the low-temperature regulation and control is performed, the root and stem of strawberry seedling are thick, the resistance property and environment adaptability are improved, and the transplanting survival rate is increased to 97%.