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Rapid propagation method for mutation breeding of typhonium giganteum

A technology of mutation breeding and unicorn lotus, applied in the field of plants, can solve eye deviation, hemiplegia, tetanus, bruises, limb numbness, stroke silence, lymphatic tuberculosis, snake bites, skin and mucous membrane irritation and other problems, to achieve the effects of large-scale planting, strong rhizomes, and high mutagenesis survival rate

Inactive Publication Date: 2015-01-07
NANJING DIDAO AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has a strong stimulating effect on the skin and mucous membranes, can expel wind and phlegm, expel cold and dampness, relieve spasm, treat headache, crooked mouth and eyes, hemiplegia, tetanus, bruises, numbness of limbs, stroke silence, lymphatic tuberculosis, snakes and insects Bite, etc., the unicorn lotus adopts tubers and seeds to reproduce, and the tissue culture method has not been reported yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Take the seeds of unicorn lotus, clean them, soak them in clear water for 5 hours, soak them in 0.1% mercuric acid solution for 15 minutes, change the water every 8 hours, after changing them three times, wash them with running water for 4 times, and then rinse them with running water for 4 times under sterile conditions. Next, sterilize the seeds with 70% ethanol for 3 minutes, then sterilize them with 0.2% mercuric chloride solution for 15 minutes, wash them with sterile water 5 times, absorb the surface moisture with absorbent paper, and place the sterilized seeds in a full-light incubator at 30°C. medium culture, the substrate is sandy loam, and the substrate is sterilized in an autoclave with a humidity of 85%. The cultivated aseptic seedlings are sprayed with sterilized colchicine at a concentration of 100mg / L, spraying once every 6 hours , the treated sterile seedlings cut off the roots and put them into medium B 5 +ZT0.5mg / L+2ip0.2mg / L for proliferative culture ...

Embodiment 2

[0011] Take the seeds of unicorn lotus, clean them, soak them in clear water for 5 hours, soak them in 0.1% mercuric acid solution for 15 minutes, change the water every 8 hours, after changing them three times, wash them with running water for 4 times, and then rinse them with running water for 4 times under sterile conditions. Next, sterilize the seeds with 70% ethanol for 3 minutes, then sterilize them with 0.2% mercuric chloride solution for 15 minutes, wash them with sterile water 5 times, absorb the surface moisture with absorbent paper, and place the sterilized seeds in a full-light incubator at 30°C. medium culture, the substrate is sandy loam, and the substrate is sterilized in an autoclave with a humidity of 85%. The cultivated aseptic seedlings are sprayed with sterilized colchicine at a concentration of 200mg / L, spraying once every 6 hours , the treated sterile seedlings cut off the roots and put them into medium B 5 +ZT0.5mg / L+2ip0.2mg / L for proliferative culture ...

Embodiment 3

[0013] Take the seeds of unicorn lotus, clean them, soak them in clear water for 5 hours, soak them in 0.1% mercuric acid solution for 15 minutes, change the water every 8 hours, after changing them three times, wash them with running water for 4 times, and then rinse them with running water for 4 times under sterile conditions. Next, sterilize the seeds with 70% ethanol for 3 minutes, then sterilize them with 0.2% mercuric chloride solution for 15 minutes, wash them with sterile water 5 times, absorb the surface moisture with absorbent paper, and place the sterilized seeds in a full-light incubator at 30°C. medium culture, the substrate is sandy loam, and the substrate is sterilized in an autoclave with a humidity of 85%. The cultivated aseptic seedlings are sprayed with sterilized colchicine at a concentration of 300 mg / L, spraying once every 6 hours , the treated sterile seedlings cut off the roots and put them into medium B 5 +ZT0.5mg / L+2ip0.2mg / L for proliferative culture...

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PUM

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Abstract

The invention discloses a rapid propagation method for mutation breeding of typhonium giganteum. The rapid propagation method comprises obtaining of sterile typhonium giganteum seeds, polyploidy induction, proliferation of cluster buds, root induction and the like. The rapid propagation method is combined with a tissue culture method for mutation breeding research on the typhonium giganteum so as to obtain a novel variety of the typhonium giganteum with large and thick leaves, thick and strong rhizomes and high unitization rate of officinal parts.

Description

technical field [0001] The invention relates to a rapid propagation method for tissue culture of unicorn lotus, belonging to the technical field of plants. Background technique [0002] unicorn, TypHonium giganteum Engl. , Araceae, dripping ginseng, Araceae, wild taro, white aconite, Yubaifu, furudinous bean, taro leaf pinellia, born in wasteland, hillsides, beside ditches, usually below 1500 meters above sea level, likes a warm and humid climate, can Cold-resistant, shade-resistant, drought-resistant, sandy soil can be planted. Species endemic to China, produced in Jilin, Liaoning, Hebei, Shandong, Henan, Hunan, Hubei, Shaanxi, Gansu, Sichuan and southern Tibet. It is best used wild in Changbai Mountain for medicinal purposes, and cultivated in Liaoning, Jilin, Guangdong and Guangxi. Unicorn lotus likes a cool and humid environment. The soil is suitable for sandy loam with good drainage. It can grow in general black loam, sandy loam, flat land, slope land and mountain lan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘东锋杨成东
Owner NANJING DIDAO AGRI SCI & TECH
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