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A kind of lignite bio-fertilizer and production method thereof

A bio-fertilizer and bio-organic fertilizer technology, which is applied in the fields of fertilization methods, botany equipment and methods, and bio-organic part treatment, can solve problems such as production research that has not been reported, and achieve obvious yield, obvious effect, and bright green leaves Effect

Active Publication Date: 2015-09-09
XINJIANG KELIN SIDE NEW ENERGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there are many reports and studies on the production of lignite bio-fertilizer by using Phanerochaete chrysosporium, Bacillus, etc., but using Davidiella tassiana and Streptomyces griseoplanus) There is no report on the production of lignite bio-fertilizer by strains

Method used

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  • A kind of lignite bio-fertilizer and production method thereof
  • A kind of lignite bio-fertilizer and production method thereof
  • A kind of lignite bio-fertilizer and production method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Embodiment one: the preparation of lignite bio-organic fertilizer

[0026] %According to the weight percentage, using lignite as the raw material and corn stalks and cotton stalks as auxiliary materials, the lignite is crushed to less than 50 mesh, the length of the auxiliary materials is crushed to less than 2mm, and the auxiliary materials are added to the coal powder to form the ingredients. The ratio is lignite 40%-50%, 20%-25% corn straw, 10%-15% cotton straw, adjust the moisture to 30-50%, and then put Davidiella Tassiana CGMCC No.7440 and Streptomyces griseus ( Streptomyces griseoplanus) CGMCC No.8194 fermented liquid was put into the above ingredients by 10% by v / w, stirred evenly, and the pH of the ingredients was adjusted to 6.5 to 7.5. After 5-7 days of incubation and fermentation at 28°C, it was dried and pulverized to obtain lignite bio organic fertilizer.

[0027] The lignite bio-organic fertilizer obtained by the above preparation method, % is calcula...

Embodiment 2

[0030] Embodiment two: Davidiella Tassiana Isolation, screening, classification and identification of CGMCC No. 7440

[0031] (1) Isolation and screening of strains

[0032] Through the separation, screening and cultivation of coal samples in the coal mine area of ​​Fukang City, Xinjiang, a batch of microbial strains that can degrade low-rank coal in Xinjiang were obtained. After further screening, the specific steps of screening are: take 1g of mine coal samples, Add sterile water and dilute to 10 0 、10 -1 and 10 -2 Three gradient concentrations were evenly spread on the CDA solid medium, and cultured at 25°C. After the colonies had fully grown, colonies of different shapes were selected and reinoculated into the CDA solid medium for purification. Repeated purification for 5 generations, after After plate screening, 7 lignite-degrading strains were isolated; after further re-screening, the degradation strain RM-1 was obtained. A strain numbered RM-1 was screened out, an...

Embodiment 3

[0042] Embodiment three: streptomyces griseus ( Streptomyces griseoplanus ) Isolation, screening, classification and identification of CGMCC No.8194

[0043] (1) Isolation and screening of strains: through the separation, screening and cultivation of coal samples in the Shawan coal mine area of ​​Xinjiang, a batch of microbial strains that can degrade low-rank coal in Xinjiang were obtained, and the specific steps of screening were further screened Yes: Take 1g of mine coal sample, add sterile water, dilute to three gradient concentrations of 100, 10-1 and 10-2, spread evenly on NA solid medium, 28 ℃, static culture, until the colony After sufficient growth, colonies of different shapes were selected and re-inoculated into NA solid medium for purification. In this way, the purification was repeated for 5 generations. After plate screening, a total of 7 lignite-degrading strains were isolated; after further re-screening, the degradation strain RM-201 was obtained. A strain nu...

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Abstract

The invention discloses a lignite biological fertilizer and a production method thereof. A strain with stronger liquidation activity is obtained through screening low-rank coal degrading bacteria in coal samples in coal mines of Fukang city and Shawan in Sinkiang, and (1) Davidiellatassiana ( / i) CGMCC No.7440 and (i) Streptomucesgriseoplanus ( / i) CGMCC No.8194 are obtained through further screening and domestication breeding. Through determination and analysis of morphological characteristics and corresponding sequences of the obtained strain, the taxonomic status and the attribute are preliminarily determined; low-rack coal is degraded by using the obtained two strains, degraded lignite is obtained by using lignite and agricultural leftovers such as straws as auxiliary materials, so that a biological organic fertilizer is produced and has a stable and remarkable technical effect of increasing yield and income when being used in cotton and rice planting through experimental crop planting.

Description

technical field [0001] The invention relates to the technical field of microbial fertilizer preparation, in particular to the technical field of application of bio-fertilizer produced by using microorganisms to degrade lignite and a production method thereof. Background technique [0002] China is rich in lignite resources. Lignite is a kind of low-rank coal. Low-rank coal is coal in the low metamorphic stage. According to the classification of coal seam coal in China GB / T17607, low-rank coal is defined as coal with Qgr.maf<24 MJ / kg. Lignite is a young coal type that is easy to weather and spontaneously combust, has low calorific value, high water content, oxygen content and volatile matter, has low aromatic carbon content, and contains a small amount of elements such as nitrogen and sulfur. Direct combustion of lignite has low thermal efficiency and low industrial application value. Long-term open-air stacking not only causes energy waste, but also easily causes environ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05F17/00C05F15/00A01G16/00A01G1/00A01C21/00A01G22/22
CPCY02W30/43Y02W30/40
Inventor 郜琳杨雪松何茂史应武冯晓霄高雁杨红梅张涛尤陆花
Owner XINJIANG KELIN SIDE NEW ENERGY
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